DOI QR코드

DOI QR Code

Draft Genome Sequences of a Unique t324-ST541-V Methicillin-Resistant Staphylococcus aureus Strain from a Pig

  • Moon, Dong Chan (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Byung-Yong (ChunLab Inc., Seoul National University) ;
  • Nam, Hyang-Mi (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Jang, Geum-Chan (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Jung, Suk-Chan (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Hee-Soo (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Park, Yong-Ho (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Lim, Suk-Kyung (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • Received : 2015.10.07
  • Accepted : 2015.12.23
  • Published : 2016.04.28

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA), the major causative agent of nosocomial infection, has also been reported from non-human sources. A sequence type (ST) 541 MRSA isolate designated K12PJN53 was isolated from a healthy pig in 2012. The genome of K12PJN53 consists of 44 contiguous sequences (contigs), totalling 2,880,108 bases with 32.88% GC content. Among the annotated contigs, 14, 17, and 18 contained genes related to antimicrobial resistance, adherence, and toxin genes, respectively. The genomic distance of strain K12PJN53 was close to the ST398 strains. This is the first report of the draft genome sequence of a novel livestock-associated MRSA ST541 strain.

Keywords

References

  1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997 . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. Benito D, Lozano C, Gomez-Sanz E, Zarazaga M, Torres C. 2013. Detection of methicillin-susceptible Staphylococcus aureus ST398 and ST133 strains in gut microbiota of healthy humans in Spain. Microb. Ecol. 66: 105-111. https://doi.org/10.1007/s00248-013-0240-1
  3. Delcher AL, Bratke KA, Powers EC, Salzberg SL. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23: 673-679. https://doi.org/10.1093/bioinformatics/btm009
  4. Disz T, Akhter S, Cuevas D, Olson R, Overbeek R, Vonstein V, et al. 2010. Accessing the SEED genome databases via Web services API: tools for programmers. BMC Bioinformatics 11: 319. https://doi.org/10.1186/1471-2105-11-319
  5. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38: 1008-1015.
  6. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM. 2007. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int. J. Syst. Evol. Microbiol. 57: 81-91. https://doi.org/10.1099/ijs.0.64483-0
  7. Hung WC, Takano T, Higuchi W, Iwao Y, Khokhlova O, Teng LJ, Yamamoto T. 2012. Comparative genomics of community-acquired ST59 methicillin-resistant Staphylococcus aureus in Taiwan: novel mobile resistance structures with IS1216V. PLoS One 7: e46987. https://doi.org/10.1371/journal.pone.0046987
  8. Jones TF, Kellum ME, Porter SS, Bell M, Schaffner W. 2002. An outbreak of community-acquired foodborne illness caused by methicillin-resistant Staphylococcus aureus. Emerg. Infect. Dis. 8: 82-84. https://doi.org/10.3201/eid0801.010174
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62: 716-721. https://doi.org/10.1099/ijs.0.038075-0
  10. Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H, Marvig RL, et al. 2012. Multilocus sequence typing of total-genome-sequenced bacteria. J. Clin. Microbiol. 50: 1355-1361. https://doi.org/10.1128/JCM.06094-11
  11. Lee JH, Yi H, Chun J. 2011. rRNASelector: a computer program for selecting ribosomal RNA encoding sequences from metagenomic and metatranscriptomic shotgun libraries. J. Microbiol. 49: 689-691. https://doi.org/10.1007/s12275-011-1213-z
  12. Lim SK, Nam HM, Jang GC, Lee HS, Jung SC, Kwak HS. 2012. The first detection of methicillin-resistant Staphylococcus aureus ST398 in pigs in Korea. Vet. Microbiol. 155: 88-92. https://doi.org/10.1016/j.vetmic.2011.08.011
  13. Mason WJ, Blevins JS, Beenken K, Wibowo N, Ojha N, Smeltzer MS. 2001. Multiplex PCR protocol for the diagnosis of staphylococcal infection. J. Clin. Microbiol. 39: 3332-3338. https://doi.org/10.1128/JCM.39.9.3332-3338.2001
  14. Moon DC, Tamang MD, Nam HM, Jeong JH, Jang GC, Jung SC, et al. 2015. Identification of livestock-associated methicillin-resistant Staphylococcus aureus isolates in Korea and molecular comparison between isolates from animal carcasses and slaughterhouse workers. Foodborne Pathog. Dis. 12: 327-334. https://doi.org/10.1089/fpd.2014.1868
  15. Pantosti A. 2012. Methicillin-resistant Staphylococcus aureus associated with animals and its relevance to human health. Front. Microbiol. 3: 127. https://doi.org/10.3389/fmicb.2012.00127
  16. Tatusov RL, Koonin EV, Lipman DJ. 1997. A genomic perspective on protein families. Science 278: 631-637. https://doi.org/10.1126/science.278.5338.631
  17. Verkade E, Kluytmans J. 2014. Livestock-associated Staphylococcus aureus CC398: animal reservoirs and human infections. Infect. Genet. Evol. 21: 523-530. https://doi.org/10.1016/j.meegid.2013.02.013

Cited by

  1. Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution vol.10, pp.1, 2016, https://doi.org/10.1186/s13073-018-0514-9
  2. Prevalence and Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates in Pigs and Pig Farmers in Korea vol.16, pp.4, 2016, https://doi.org/10.1089/fpd.2018.2509