대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)

  • 제48권2호
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  • Pages.62-67
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  • 2016
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  • 1738-3544(pISSN)
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  • 2288-1662(eISSN)
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)
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제주 자생 조록나무 분획물의 항산화 효과

Antioxidant Activity of Solvent Fractions from Distylium racemosum in Jeju

  • 김혜란 (부산가톨릭대학교 임상병리학과) ;
  • 박규남 (부산가톨릭대학교 임상병리학과) ;
  • 정보경 (부산가톨릭대학교 임상병리학과) ;
  • 윤원종 (제주테크노파크 생물종다양성연구소) ;
  • 정용환 (제주테크노파크 생물종다양성연구소) ;
  • 장경수 (부산가톨릭대학교 임상병리학과)
  • Kim, Hye-Ran (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Park, Gyu-Nam (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Jung, Bo-Kyoung (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, Catholic University of Pusan)
  • 투고 : 2016.02.23
  • 심사 : 2016.03.07
  • 발행 : 2016.06.30
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초록

600여 종의 제주 천연 추출물을 이용하여 항산화 효과를 스크린한 결과, 조록나무 잎 추출물이 가장 높은 항산화 효과를 나타내었다. 조록나무는 조록나무과에 속하는 상록 나무로써 제주 천연자원이다. 본 연구에서는 극성이 다른 유기용매(헥산, 메틸렌 클로라이드, 에틸 아세테이트, 부탄올, 물)로 순차적으로 분획 한 조록나무 물질 5가지를 이용하여 항산화 효과 및 간세포와 폐세포를 이용하여 세포 보호능을 확인하였다. 에틸 아세테이트 분획물질은 0.5 mg/mL에서 약93%로 가장 우수한 DPPH 라디칼 소거능을 보이며 양성 대조군으로 사용한 quercetin 보다 높은 효능을 보였다. 총 페놀 함량을 확인 한 결과, 에틸 아세테이트 분획물질은 505 mg GAE/g로 가장 높은 총 페놀 함량을 보였으며, 모든 추출물에서 DPPH 라디칼 소거능과 연관성 있는 결과를 보였다. 또한 에틸 아세테이트 분획물질은 $H_2O_2$에 대한 산화적 스트레스로부터 다른 분획 물질보다 높은 세포보호효과를 나타내었다. 본 연구를 통해 조록나무의 에틸 아세테이트 분획물질의 높은 항산화 효과를 확인하였으며, 천연 항산화 소재의 가능성이 높을 것으로 사료된다.

Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide ($H_2O_2$) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.

키워드

참고문헌

  1. Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chemistry. 2004;85:611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
  2. Bayati S, Yazdanparast R. Antioxidant and free radical scavenging potential of yakuchinone B derivatives in reduction of lipofuscin formation using $H_2O_2$-treated neuroblastoma cells. Iran Biomed J. 2011;15:134-142.
  3. Kumarappan CT, Thilagam E, Mandal SC. Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens. Saudi J Biol Sci. 2012;19:349-355. https://doi.org/10.1016/j.sjbs.2012.04.004
  4. Debnath T, Park SR, Kim DH, Jo JE, Lim BO. Anti-oxidant and anti-inflammatory activities of Inonotus obliquus and germinated brown rice extracts. Molecules. 2013;18:9293-9304. https://doi.org/10.3390/molecules18089293
  5. Mahakunakorn P, Tohda M, Murakami Y, Matsumoto K, Watanabe H. Antioxidant and free radical-scavenging activity of Choto-san and its related constituents. Biol Pharm Bull. 2004;27:38-46. https://doi.org/10.1248/bpb.27.38
  6. Chipiti T, Ibrahim MA, Koorbanally NA, Islam MS. In vitro antioxidant activities of leaf and root extracts of Albizia antunesiana harms. Acta Pol Pharm. 2013;70:1035-1043.
  7. Lampe JW. Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies. Am J Clin Nutr. 1999;70(3 Suppl):S475-490. https://doi.org/10.1093/ajcn/70.3.475s
  8. Moon JY, Yim EY, Song G, Lee NH, Hyun CG. Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants. EurAsia J. BioSci. 2010;4:41-53.
  9. Ko RK, Lee S, Hyun CG, Lee NH. New dibenzofurans from the branches of Distylium racemosum Sieb. et Zucc. Bull. Korean Chem. Soc. 2009;30:1376-1378. https://doi.org/10.5012/bkcs.2009.30.6.1376
  10. Ko RK, Kim GO, Hyun CG, Jung DS, Lee NH. Compounds with tyrosinase inhibition, elastase inhibition and DPPH radical scavenging activities from the branches of Distylium racemosum Sieb. et Zucc. Phytother Res. 2011;25:1451-1456. https://doi.org/10.1002/ptr.3439
  11. Kim JA, Yang SY, Wamiru A, McMahon JB, Le Grice SF, Beutler JA, et al. New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H. Bioorg Med Chem Lett. 2011;21:2840-2844. https://doi.org/10.1016/j.bmcl.2011.03.091
  12. Lee SM, Na MK, An RB, Min BS, Lee HK. Antioxidant activity of two phloroglucinol derivatives from Dryopteris crassirhizoma. Biol Pharm Bull. 2003;26:1354-1356. https://doi.org/10.1248/bpb.26.1354
  13. Jo HJ, Shin YS, Park GN, Chang KS. Analysis of anti-oxidant activity of medicinal plants according to the extracted parts. Journal of Medicinal Plants Research. 2013;7:1089-1099.
  14. Duthie G, Morrice P. Antioxidant capacity of flavonoids in hepatic microsomes is not reflected by antioxidant effects in vivo. Oxid Med Cell Longev. 2012;2012:165127.
  15. Jullian C, Moyano L, Yanez C, Olea-Azar C. Complexation of quercetin with three kinds of cyclodextrins: an antioxidant study. Spectrochim Acta A Mol Biomol Spectrosc. 2007;67:230-234. https://doi.org/10.1016/j.saa.2006.07.006
  16. Ferrer P, Asensi M, Segarra R, Ortega A, Benlloch M, Obrador E, et al. Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma. Neoplasia. 2005;7:37-47. https://doi.org/10.1593/neo.04337
  17. Kim KM, Kim YS, Lim JY, Min SJ, Ko HC, Kim SJ, et al. Intestinal anti-inflammatory activity of Sasa quelpaertensis leaf extract by suppressing lipopolysaccharide-stimulated inflammatory mediators in intestinal epithelial Caco-2 cells co-cultured with RAW 264.7 macrophage cells. Nutr Res Pract. 2015;9:3-10. https://doi.org/10.4162/nrp.2015.9.1.3
  18. Piao MJ, Hyun YJ, Cho SJ, Kang HK, Yoo ES, Koh YS, et al. An ethanol extract derived from Bonnemaisonia hamifera scavenges ultraviolet B (UVB) radiation-induced reactive oxygen species and attenuates UVB-induced cell damage in human keratinocytes. Mar Drugs. 2012;10:2826-2845. https://doi.org/10.3390/md10122826
  19. Hong JH, Lee IS. Effects of Artemisia capillaris ethyl acetate fraction on oxidative stress and antioxidant enzyme in high-fat diet induced obese mice. Chem Biol Interact. 2009;179:88-93. https://doi.org/10.1016/j.cbi.2008.12.002
  20. Seo YM, Park ST, Jekal SJ, Kim SM, Rim YS. A study on the physioactivities of Salicornia herbacea L. grown in Sunchon bay on cell viability and antioxidative effect in cultured C6 glioma Cells. Korean J Clin Lab Sci. 2011;43:98-104.
  21. Chen CJ, Deng AJ, Liu C, Shi R, Qin HL, Wang AP. Hepatoprotective activity of Cichorium endivia L. extract and its chemical constituents. Molecules. 2011;16:9049-9066. https://doi.org/10.3390/molecules16119049
  22. Won SB, Jung GY, Kim J, Chung YS, Hong EK, Kwon YH. Protective effect of Pinus koraiensis needle water extract against oxidative stress in HepG2 cells and obese mice. J Med Food. 2013;16:569-576. https://doi.org/10.1089/jmf.2012.2665
  23. Jekal SJ, Min BW, Park H. Protective effects of Curcumin on CCl4-induced hepatic fibrosis with high fat diet in C57BL/6 mice. Korean J Clin Lab Sci. 2015;47:251-258. https://doi.org/10.15324/kjcls.2015.47.4.251

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  1. 심비디움 뿌리 및 줄기 추출물의 생리 활성 vol.33, pp.4, 2016, https://doi.org/10.12925/jkocs.2016.33.4.848
  2. 탁리산 용매 분획물의 in vitro 항산화, 주름 억제 및 미백 효과 vol.60, pp.3, 2016, https://doi.org/10.3839/jabc.2017.035
  3. 큰개불알풀 추출물의 항산화 및 항염증 기능성 평가 vol.26, pp.4, 2016, https://doi.org/10.11625/kjoa.2018.26.4.661
  4. Anti-inflammatory Effect of Distylium racemosum leaf Biorenovate Extract in LPS-stimulated RAW 264.7 Macrophages Cells vol.64, pp.4, 2021, https://doi.org/10.3839/jabc.2021.051