Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

  • You, Jungmok (Department of Plant & Environmental New Resources, Kyung Hee University) ;
  • Heo, June Seok (Cell Therapy Center, Severance Hospital, Yonsei University College of Medicine) ;
  • Kim, Hyun Ok (Cell Therapy Center, Severance Hospital, Yonsei University College of Medicine) ;
  • Kim, Eunkyoumg (Department of Chemical and Biomolecular Engineering, Yonsei University)
  • Received : 2016.06.08
  • Accepted : 2016.07.26
  • Published : 2016.09.01
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Abstract

Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRT), Korea Health Industry Development Institute (KHIDI)

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