생체재료학회지 (Biomaterials Research)

  • 제20권1호
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  • Pages.8-14
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  • 2016
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds

  • Kim, Min Jeong (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University) ;
  • Shin, Yong Cheol (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University) ;
  • Lee, Jong Ho (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University) ;
  • Jun, Seung Won (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University) ;
  • Kim, Chang-Seok (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University) ;
  • Lee, Yunki (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Jong-Chul (Department of Medical Engineering, Cellbiocontrol Laboratory, Yonsei University College of Medicine) ;
  • Lee, Soo-Hong (Department of Biomedical Science, CHA University) ;
  • Park, Ki Dong (Department of Molecular Science and Technology, Ajou University) ;
  • Han, Dong-Wook (Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University)
  • 투고 : 2015.11.04
  • 심사 : 2016.01.04
  • 발행 : 2016.03.01
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM. Results: The physicochemical properties of GHPA hydrogels were characterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. In addition, the cell viability and proliferation of C2C12 myoblasts cultured in the GHPA hydrogels were analyzed by using Live/Dead Cell and CCK-8 assays, respectively. It was found that C2C12 cells were well grown and normally proliferated in the hydrogels. Furthermore, the hydrogels were shown to be suitable to facilitate the myogenic differentiation of C2C12 cells incubated in differentiation media, which had been corroborated by MPM. It was very hard to get clear images from a fluorescence microscope. Conclusions: Our findings suggest that the gelatin-based hydrogels can be beneficially utilized as 3D scaffolds for skeletal muscle engineering and that MPM can be effectively applied to imaging technology for tissue regeneration.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

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  1. Synthesis and Biocompatibility Characterizations of in Situ Chondroitin Sulfate-Gelatin Hydrogel for Tissue Engineering vol.15, pp.1, 2016, https://doi.org/10.1007/s13770-017-0089-3
  2. Three-dimensional graphene oxide-coated polyurethane foams beneficial to myogenesis vol.29, pp.7, 2016, https://doi.org/10.1080/09205063.2017.1348738
  3. Three-Dimensional Printable Gelatin Hydrogels Incorporating Graphene Oxide to Enable Spontaneous Myogenic Differentiation vol.10, pp.None, 2016, https://doi.org/10.1021/acsmacrolett.0c00845
  4. Advances in gelatin-based hydrogels for wound management vol.9, pp.6, 2016, https://doi.org/10.1039/d0tb02582h
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