Analysis of Vitamin E in Agricultural Processed Foods in Korea

국내 농산가공식품의 비타민 E 함량 분석

Accurate food composition data are essential for calculation of nutrient intake of a population based on its consumption statistics. In the Korean food composition database, there is a lack of reliable analytical data for tocopherols and tocotrienols. Therefore, this study was conducted to provide information on contents on vitamin E in agricultural processed foods in Korea. Tocopherols and tocotrienols were determined by the saponification extraction method followed by high performance liquid chromatography. Analytical method validation parameters were calculated to ensure the method's validity. Samples were obtained in the years of 2013 and 2014 from the Rural Development Administration. The samples included 34 grains and grain products, 14 snacks, 25 fruits, 5 oils, and 11 sources and spices. All vitamin E isomers were quantitated, and the results were expressed as ${\alpha}$-tocopherol equivalent (${\alpha}-TE$). ${\alpha}-TE$ values of cereal and cereal products, snacks, fruits, oils and sauces and spices ranged from 0.03 to 17.53, 1.01 to 12.84, 0.01 to 1.52, 1.09 to 8.15, and 0.01 to $27.53{\alpha}-TE/100g$, respectively. Accuracy was close to 100% (n=3). Repeatability and reproducibility were 2.04% and 4.69%, respectively. Our study provides reliable data on the tocopherol and tocotrienol contents of agricultural and processed foods in Korea.

본 연구에서는 국내 농산가공식품의 비타민 E 함량을 측정하여 각 식품군에서 비타민 E 함량을 분석하고 이에 대한 분석법을 검증하고자 하였다. 곡류 및 곡류 가공품에서는 쌀이 첨가된 시리얼이 $17.53{\alpha}-TE/100g$으로 가장 높은 값을 나타내었으며, 과자류에서는 땅콩 샌드가 $12.84{\alpha}-TE/100g$으로, 과일류에서는 골드키위가 $1.52{\alpha}-TE/100g$으로 가장 높은 값을 나타내었다. 유지류에서는 들기름이 $8.15{\alpha}-TE/100g$으로 가장 높은 값을 나타내었으며 소스 및 향신료에서는 고춧가루가 $27.53{\alpha}-TE/100g$으로 가장 높은 값을 나타내었다. 분석방법을 검증하기 위하여 정확성과 정밀성을 측정하였으며 분석관리차트를 작성하여 결과 값을 관리하였다. 표준참고물질을 사용하여 측정한 정확성은 각각 99.99%, 90.70%로 나타내었고, 정밀성은 반복성이 2.04%, 재현성이 4.69%로 우수한 값을 나타내어 본 실험방법이 적합한 시험법임이 검증되었다.