Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Gamma Irradiation on Wood Chip Saccharification Pretreated with NaOH

NaOH를 이용한 우드칩의 당화 전처리에 대한 감마선 조사 영향 연구

  • Kim, Su-mi (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University) ;
  • Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University) ;
  • Joe, Min-Ho (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jong-deog (Department of Biotechnology, Chonnam National University)
  • 김수미 (전남대학교 생물공학과 바이오에너지및바이오소재 협동과정) ;
  • 최종일 (전남대학교 생물공학과 바이오에너지및바이오소재 협동과정) ;
  • 조민호 (한국원자력연구원 첨단방사선연구소) ;
  • 김종덕 (전남대학교 생명산업공학과)
  • Received : 2015.11.04
  • Accepted : 2016.01.09
  • Published : 2016.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate the effect of gamma irradiation on the pretreatment of wood chips with NaOH solution. The degree of saccharification was quantified by measuring reducing sugar and glucose concentrations after enzymatic hydrolysis. After pretreatment with 10 g/L NaOH, the wood chips were irradiated at the doses of 0, 50, 100, and 200 kGy, respectively. Among the irradiated samples, wood chips irradiated at the dose of 200 kGy had the highest reducing sugar concentration of 12.2 g/L. Also, to define the effect of irradiation before pretreatment, the wood chips were first gamma-irradiated and then pretreated with NaOH. When the NaOH treatment was conducted after irradiation at 200 kGy, the reducing sugar content was further increased to 13.4 g/L and glucose content of the wood chip was as high as 7.9 g/L. These results suggest that gamma irradiation may be the promising method for pretreatment of cellulose biomass.

본 논문에서는 감마선 조사가 NaOH 전처리를 이용한 우드칩의 당화에 미치는 영향을 살펴보았다. 당화량은 전처리된 우드칩에 효소를 이용하여 가수분해한 후 생성된 환원당과 포도당 양을 측정하여 확인하였다. 10 g/L NaOH 처리한 우드칩을 각각 50, 100, 200 kGy 세기의 감마선을 조사하여 당화량을 측정하였다. 우드칩의 가수분해 수율을 비교했을 때, 200 kGy 감마선 조사한 우드칩에서 가장 높은 환원당 함량 12.2 g/L이 얻어졌다. 또한 전처리 과정에서의 감마선 조사 단계의 영향을 평가하기 위하여 우드칩을 먼저 50, 100, 200 kGy 감마선 조사한 후, 10 g/L NaOH 로 전처리하였다. 200 kGy 감마선 조사 후 NaOH 전처리를 수행했을 때 환원당 함량은 13.4 g/L로 전처리 이후의 감마선 조사구보다 증가하였고, 7.9 g/L 포도당을 얻을 수 있었다. 이러한 결과로부터 감마선 조사가 셀루로오즈 바이오매스 전처리에 활용 가능할 것으로 기대된다.

Keywords

References

  1. Zhang, L., Chen, L., Wang, J., Chen, Y., Gao, X., Zhang, Z. and Liu, T., "Attached Cultivation for Improving the Biomass Productivity of Spirulina platensis," Bioresource Technology, 181, 136-142(2015). https://doi.org/10.1016/j.biortech.2015.01.025
  2. Ryu, J. S., Kim, K. S. and Park, S. J., "A Study on Combustion Characteristics of Wood Biomass for Cogeneration Plant," Applied Chemistry for Engineering, 22, 296-300(2011).
  3. McKendry, P., "Energy Production from Biomass (part 1): Overview of Biomass," Bioresource Technology, 83(1), 37-46(2002). https://doi.org/10.1016/S0960-8524(01)00118-3
  4. McKendry, P., "Energy Production from Biomass (part 2): Conversion Technologies," Bioresource Technology, 83(1), 47-54(2002). https://doi.org/10.1016/S0960-8524(01)00119-5
  5. Fortier, J., Truax, B., Gagnon, D. and Lambert, F., "Biomass Carbon, Nitrogen and Phosphorus Stocks in Hybrid Poplar Buffers, Herbaceous Buffers and Natural Woodlots in the Riparian Zone on Agricultural Land," Journal of Environmental Management, 154, 333-345(2015). https://doi.org/10.1016/j.jenvman.2015.02.039
  6. Hoekman, S. K., "Biofuels in the U.S. - Challenges and Opportunities," Renewable Energy, 34, 14-22(2009). https://doi.org/10.1016/j.renene.2008.04.030
  7. Liu, S., "A Synergetic Pretreatment Technology for Woody Biomass Conversion," Applied Energy, 144, 144-128(2015). https://doi.org/10.1016/j.apenergy.2015.02.021
  8. Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y. Y., Holtzapple, M. and Ladisch, M., "Features of Promising Technologies for Pretreatment of Lignocellulosic Biomass," Bioresource Technology, 96, 673-686(2005). https://doi.org/10.1016/j.biortech.2004.06.025
  9. Toscano, G., Duca, D., Rossini, G., Mengarelli, C. and Pizzi, A., "Identification of Different Woody Biomass for Energy Purpose by Means of Soft Independent Modeling of Class Analogy Applied to Thermogravimetric Analysis," Energy, 83, 351-357(2015). https://doi.org/10.1016/j.energy.2015.02.032
  10. Gregg, D. and Saddler, J. N., "A Techno-economic Assessment of the Pretreatment and Fractionation Steps of a Biomass-to-ethanol Process," Applied Biochemistry and Biotechnology, 57/58, 711- 727(1996). https://doi.org/10.1007/BF02941753
  11. Si, S., Chen, Y., Fan, C., Hu, H., Li, Y., Huang, J., Liao, H., Hao, B., Li, Q., Peng, L. and Tu, Y., "Lignin Extraction Distinctively Enhances Biomass Enzymatic Saccharification in Hemicellulosesrich Miscanthus Species Under Various Alkali and Acid Pretreatments," Bioresource technology, 183, 248-254(2015). https://doi.org/10.1016/j.biortech.2015.02.031
  12. Hendriks, A. T. W. M. and Zeeman, G., "Pretreatments to Enhance the Digestibility of Lignocellulosic Biomass," Bioresource Technology, 100(1), 10-18(2009). https://doi.org/10.1016/j.biortech.2008.05.027
  13. Zheng, Y., Pan, Z., Zhang, R. and Wang, D., "Enzymatic Saccharification of Dilute Acid Pretreated Saline Crops for ferMentable Sugar Production," Applied Energy, 86(11), 2459-2465(2009). https://doi.org/10.1016/j.apenergy.2009.03.012
  14. MacDonald, D. G., Bakhshi, N. N., Mathews, J. F., Roychowdhury, A., Bajpai, P. and Moo-Young, M., "Alkali Treatment of Corn Stover to Improve Sugar Production by Enzymatic Hydrolysis," Biotechnology and Bioengineering, 25(8), 2067-2076(1983). https://doi.org/10.1002/bit.260250815
  15. Xu, N., Zhang, W., Ren, S., Liu, F., Zhao, C., Liao, H., Xu, Z., Huang, J., Li, Q., Tu, Y., Yu, B., Wang, Y., Jiang, J., Qin, J. and Peng, L., "Hemicelluloses Negatively Affect Lignocellulose Crystallinity for High Biomass Digestibility Under NaOH and $H_2SO_4$ Pretreatments in Miscanthus," Biotechnolgy for Biofuels, 5(1), 58(2012). https://doi.org/10.1186/1754-6834-5-58
  16. Sen, M., Toprak, D. and Guven, O., "The Effect of Environmental Humidity on Radiation-induced Degradation of Carrageenans," Carbohydrate Polymers, 114, 546-552(2014). https://doi.org/10.1016/j.carbpol.2014.08.031
  17. Lee, J. S., "Status and Prospects of Cellulosic Ethanol R&D," Korean Information Center News, 16, 38-48(2013).
  18. Choi, J. I., "Structural Characteristics of Low Molecular Weight Laminarin Prepared by Ionizing Irradiation," Korean Chemical Engineering Research, 51, 780-783(2013). https://doi.org/10.9713/kcer.2013.51.6.780
  19. Binod, P., Sindhu, R., Singhania, R. R., Vikram, S., Devi, L., Nagalakshmi, S., Kurien, N., Sukumaran, R. K., Pandey, A., "Bioethanol Production from Rice Straw: an Overview," Bioresource Technology, 101(13), 4767-4774(2010). https://doi.org/10.1016/j.biortech.2009.10.079
  20. Lee, J. Y., Lee, B. M., Jeun, J. P. and Kang, P. H., "Pretreatment of Kenaf Core by Combined Electron Beam Irradiation and Water Steam for Enhanced Hydrolysis," Korean Chemical Engineering Research, 52(1), 113-118(2014). https://doi.org/10.9713/kcer.2014.52.1.113
  21. Xin, L. Z. and Kumakura, M., "Effect of Radiation Pretreatment on Enzymatic Hydrolysis of Rice Straw with Low Concentrations of Alkali Solution," Bioresource Technology, 43(1), 13-17(1993). https://doi.org/10.1016/0960-8524(93)90076-N
  22. Bernfeld, P., "Amylases, ${\alpha}$ and ${\beta}$," Methods in Enzymology, 1, 149-158(1955). https://doi.org/10.1016/0076-6879(55)01021-5
  23. Hinman, N. D., Wright, J. D., Hogland, W. and Wyman, C. E., "Xylose Fermentation," Applied Biochemistry and Biotechnology, 20(1), 391-401(1989). https://doi.org/10.1007/BF02936498
  24. Zhao, L., Zhang, X. and Tan T., "Influence of Various Glucose/xylose Mixtures on Ethanol Production by Pachysolen Tannophilus," Biomass and Bioenergy, 32(12), 1156-1161(2008). https://doi.org/10.1016/j.biombioe.2008.02.011
  25. Zhang, Y. H. P. and Lynd, L. R., "Toward an Aggregated Understanding of Enzymatic Hydrolysis of Cellulose: Noncomplexed Cellulase Systems," Biotechnology and Bioengineering, 88(7), 797-824(2004). https://doi.org/10.1002/bit.20282

Cited by

  1. 방사선 가교된 유착방지용 Carboxymethyl Cellulose/Porcine Cartilage Acellular Matrix 수화젤 필름의 물리적 특성 및 부착 방지 평가 vol.55, pp.1, 2016, https://doi.org/10.9713/kcer.2017.55.1.34
  2. 감마선 조사 처리에 의한 결명자 줄기의 전처리와 효소가수분해 효과 vol.26, pp.2, 2016, https://doi.org/10.7783/kjmcs.2018.26.2.127