Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Genetic Identification of Spirometra decipiens Plerocercoids in Terrestrial Snakes from Korea and China

  • Jeon, Hyeong-Kyu (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Park, Hansol (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Lee, Dongmin (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Choe, Seongjun (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Kim, Kyu-Heon (Ministry of Food and Drug Safety) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine)
  • Received : 2016.01.10
  • Accepted : 2016.03.20
  • Published : 2016.04.30
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Abstract

Human sparganosis is a zoonotic disease caused by infection with larval forms (procercoid/plerocercoid) of Spirometra spp. The purpose of this study was to identify Spirometra spp. of infected snakes using a multiplex PCR assay and phylogenetic analysis of mitochondrial DNA sequence data from the spargana of terrestrial snakes obtained from Korea and China. A total of 283 snakes were obtained that included 4 species of Colubridae comprising Rhabdophis tigrinus tigrinus (n=150), Dinodon rufozonatum rufozonatum (n=64), Elaphe davidi (n=2), and Elaphe schrenkii (n=7), and 1 species of Viperidae, Agkistrodon saxatilis (n=60). The snakes were collected from the provinces of Chungbuk, Chungnam, and Gyeongbuk in Korea (n=161), and from China (n=122). The overall infection rate with spargana was 83% (235/283). The highest was recorded for D. rufozonatum rufozonatum (100%), followed by A. saxatilis (85%) and R. tigrinus tigrinus (80%), with a negative result for E. davidi (0%) and E. schrenkii (0%). The sequence identities between the spargana from snakes (n=50) and Spirometra erinaceieuropaei (KJ599680) or S. decipiens (KJ599679) control specimens were 90.8% and 99.2%, respectively. Pairwise genetic distances between spargana (n=50) and S. decipiens ranged from 0.0080 to 0.0107, while those between spargana and S. erinaceieuropaei ranged from 0.1070 to 0.1096. In this study, all of the 904 spargana analyzed were identified as S. decipiens either by a multiplex PCR assay (n=854) or mitochondrial cox1 sequence analysis (n=50).

Keywords

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