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Genetic Characteristics and Immunogenicity of Pandemic H1N1 Influenza Virus Isolate from Pig in Korea

  • Hyoung Joon Moon (Research Unit, Green Cross Veterinary Products) ;
  • Jin Sik Oh (MEDIAN Diagnostics Inc.) ;
  • Woonsung Na (Department of Pharmacy, College of Pharmacy, Korea University) ;
  • Minjoo Yeom (Department of Pharmacy, College of Pharmacy, Korea University) ;
  • Sang Yoon Han (Research Unit, Green Cross Veterinary Products) ;
  • Sung Jae Kim (Research Unit, Green Cross Veterinary Products) ;
  • Bong Kyun Park (Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, and School of Agricultural Biotechnology, BK21 Program for Veterinary Science, Seoul National University) ;
  • Dae Sub Song (Department of Pharmacy, College of Pharmacy, Korea University) ;
  • Bo Kyu Kang (Research Unit, Green Cross Veterinary Products)
  • Received : 2016.06.07
  • Accepted : 2016.09.26
  • Published : 2016.10.31
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Abstract

A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.

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References

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