The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effects of Haedoksamul-tang on Oxidative Stress and Hyperlipidemia in LPS-induced ICR Mouse

해독사물탕(解毒四物湯)이 LPS 유도 ICR mouse의 산화스트레스 및 고지혈증에 미치는 효과

  • Choi, Gyu-ho (Department of Internal Medicine of Korean Medicine, College of Korean Medicine, Dae-gu Haany University) ;
  • Jung, Yu-sun (Department of Internal Medicine of Korean Medicine, College of Korean Medicine, Dae-gu Haany University) ;
  • Shin, Hyeon-cheol (Department of Internal Medicine of Korean Medicine, College of Korean Medicine, Dae-gu Haany University)
  • 최규호 (대구한의대학교 한의과대학 내과학교실) ;
  • 정유선 (대구한의대학교 한의과대학 내과학교실) ;
  • 신현철 (대구한의대학교 한의과대학 내과학교실)
  • Received : 2016.02.15
  • Accepted : 2016.03.28
  • Published : 2016.03.31
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Abstract

Objectives: The present study was conducted to examine whether Haedoksamul-tang (HS), a traditional oriental herbal medicine, have beneficail effects on anti-inflammation and dyslipidemia in lipopolysaccharide (LPS)-induced ICR mouse. Methods: Twenty four 8-week old male ICR mouse were divided into four groups: normal untreated; LPS treatment only; HS 10 mg/kg plus LPS treatment; and HS 30 mg/kg plus LPS treatment. HS was orally administered per day for 2days. Twenty-four hours after LPS injection (10 mg/kg/day, i.p.), all the mice were sacrificed, and serological changes were evaluated. The levels of nuclear factor-${\kappa}B$ (NF-${\kappa}B$), sterol regulatory element-binding transcription protein 1 (SREBP-1) activity and cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), acetyl-CoA carboxylase a (ACCa) expression were analyzed in Western blot analysis. Results: HS inhibited oxidative stress in the liver of LPS-induced ICR mice. The LPS-induced ICR mice exhibited the increase of NF-${\kappa}B$ activity and COX-2, iNOS, TNF-a, MCP-1 expressions in the liver, while HS treatment significantly inhibited them. Moreover, The administration of HS significantly decreased the elevated serum triglyceride and down-regulated the levels of SREBP-1, ACCa in the liver of LPS-induced ICR mice. Conclusions: In conclusion, HS could have hepato-protective effects against the oxidative stress-related inflammation and abnormal lipid metabolism.

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References

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