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Anti-atopic dermatitis effects of Poncirus trifoliata Rafinesque via regulation of immune response and nerve growth factor

면역반응 및 신경성장인자 조절을 통한 지실의 항아토피 효능

  • Jung, Seon A (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University) ;
  • Choi, You Yeon (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University) ;
  • Yang, Woong Mo (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University)
  • Received : 2015.11.25
  • Accepted : 2016.03.17
  • Published : 2016.03.31

Abstract

Objectives: Poncirus trifoliata Rafinesque has been known to have anti-allergic effects in skin diseases. However, anti-atopic dermatitis effects of P. trifoliata Rafinesque have not been studied yet in skin diseases. The present study evaluated the anti-atopic dermatitis effects of P. trifoliata Rafinesque (PTR) using external treatments on AD. Methods: AD lesions were induced by the repeated application of 2, 4-Dinitrochlorobenzene (DNCB) on the shaved back of BALB/c mice. $100{\mu}{\ell}$ of PTR extracts was applied to the AD lesions for 11 days. Histological assessments, mast cells count and serum levels of IgE were analyzed. The anti-pruritic effects of PTR were examined by the change of scratching frequency and nerve growth factor (NGF) expression. In addition, the anti-inflammatory effects of PTR were examined by the expressions of Th2/Th1 cytokines and pro-inflammatory in dorsal skin. Results: Histopathological findings showed that topical application of PTR decreased the thickness of dermal and epidermal skin compared with the DNCB group. PTR also notably decreased the mast cells count and serum IgE. The scratching behavior of mice and expression of NGF were significantly reduced. In addition, PTR group significantly suppressed the IL-4, IL-6, IFN-${\gamma}$ and TNF-${\alpha}$ cytokines compared to the DNCB group. Conclusions: These results indicated that P. trifoliata Rafinesque possess anti-pruritus and anti-atopic dermatitis properties. Therefore, P. trifoliata Rafinesque might be used for treatment of pruritus and atopic dermatitis.

Keywords

References

  1. Min DL, Park EJ, Kang KH. Review of Clinical and Experimental Studies on External Application Treatment for Atopic Dermatitis in the Korean Literature. J Korean Orient Pediatr. 2013;27(1):36-49. https://doi.org/10.7778/jpkm.2013.27.1.036
  2. Park Y. Status of clinical practice on diagnosis and management of atopic dermatitis in Korea: a questionnaire survey of physicians. Allergy Asthma Respir Dis. 2013;1(3):257-265. https://doi.org/10.4168/aard.2013.1.3.257
  3. Lee YJ, Kang HY, Jeong BJ, Kim KE, Lee KY, Lee SY. Significance of atopic index in children with atopic dermatitis. Allergy Asthma Respir Dis. 1994;4(2):11-24.
  4. Choi HJ, Sim BY, Miyamoto T. Effects of Lobophytum crassum extract (MC-1) on Various Immunological Factors Related to Pathogenesis of Atopic Dermatitis in Dermatophagoides Pteronyssinus Treated NC/Nga mice. Korea J Herbol. 2015;30(1):95-101. https://doi.org/10.6116/KJH.2015.30.1.95.
  5. Yun HJ, Ko WS. Clinical Study of Atopic Dermatitis; the Classification of Oriental Medical Clinical type and Treatment. J Korean Oriental Med. 2001;22(2):10-21.
  6. Lee SH, Yun YG. Study for Treatment of Atopic Dermatitis in Oriental Medical Prescription. J Korean Med. 2006;15(1):56-69.
  7. Shin SH, Kim MB, Byun SM, Lee HS, Park SG, Ko WS, et al. A Review on External Treatment for Atopic Dermatitis in Korean Journals. J Korean Orient Med Ophthalmol Otolaryngol Dermato. 2008;21(2):80-93.
  8. Katayama I, Kohno Y, Akiyama K, Ikezawa Z, Kondo N, Tamaki K, et al. Japanese Society of Allergology. Japanese Guideline for Atopic Dermatitis. Allergol Int. 2011;60:205-220. https://doi.org/10.2332/allergolint.11-RAI-0333
  9. Boneberger S, Rupec RA, Ruzicka T. Complementary therapy for atopic dermatitis and other allergic skin diseases: facts and controversies. Clin Dermatol. 2010;28(1):57-61. https://doi.org/10.1016/j.clindermatol.2009.03.017
  10. Cha KB, Kim YS, Seol IC. Literatual study on Atopic dermatitis. Daejeon J Institute Orient Med. 2005;14(2):113-126.
  11. Kim EH, Lee BW, Kim EH. A Literal Study of the Main Symptoms of Atopic Dermatitis. J Korean Med Classic. 2009;22(1):207-228.
  12. College of korean medicine co association editorial board. Textbook of herbology. Seoul :Younglimsa. 2011:392.
  13. Aeom YD, Kim DH, Jeong JG, Shin MK, Song HJ. The Comparative Study of Fructus Immaturus Ponciri and Fructus Ponciri Effect on Allergic Re action. J Korean Orient Med. 2001;22(4):10-21.
  14. Kang BS. Korean medicine clinical allergy. Seoul: Seongbosa. 1988:57-59.
  15. Hwang KH, Lee EJ, Song BK, Kim HK. Mechanism of Inhibitory Action of Anaphylaxis by Aqueous Extract of Poncirus trifoliata. J Korean Orient Med. 1997;18(1):316-325.
  16. Williams HC. Clinical practice. Atopic dermatitis. N Engl J Med. 2005;352(22):2314-2324. https://doi.org/10.1056/NEJMcp042803
  17. Cho JB, Lee JH, Suh JM, Yu JS, Lee HS, Park EA, et al. Change in Quality of Life according to the Change in Atopic Dermatitis Severity. Allergy Asthma Respir Dis. 2012;22(1):86-99. https://doi.org/10.7581/pard.2012.22.1.86
  18. Carstens E, Akiyama T. Itch: Mechanisms and Treatment. Boca Raton (FL):CRC Press/Taylor & francis. 2014. Ebook-Chapter 3.
  19. Lee YJ, Kang HY, Jeng BJ, Kim GE, Lee GY, Lee SY. Significance of atopic index in children with atopic dermatitis. Allergy Asthma Respir Dis. 1994;4(2):11-24.
  20. Leung DY, Bieber T. Atopic dermatitis. Lancet Diabetes Endocrinol. 2003;361:151-160.
  21. Hong SJ. Review Articles: Mast Cells and Allergic Diseases. Allergy Asthma Respir Dis. 2000;10(3):207-217.
  22. Hwang SY, Lee SH, Lee CW, Kim JH, Jang SI, Kim AN, et al. Anti-inflammatory and Anti-Pruritonic Effects of WSY-1075 composited with Medicinal Plants on the Activated Rat Peritoneal Mast cells and Mouse Pruritus. Korea J Herbol. 2013;28(4):93-100. https://doi.org/10.6116/kjh.2013.28.4.93
  23. Metz M, Grundmann S, Stander S. Pruritus: an overview of current concepts. Vet Dermatol. 2011;22(2):121-131. https://doi.org/10.1111/j.1365-3164.2010.00945.x
  24. Yamaguchi J, Aihara M, Kobayashi Y, Kambara T, Ikezawa Z. Quantitative analysis of nerve growth factor (NGF) in the atopic dermatitis and psoriasis horny layer and effect of treatment on NGF in atopic dermatitis. J Dermatol Sci. 2009;53(1):48-54. https://doi.org/10.1016/j.jdermsci.2008.08.011
  25. Pincelli C, Marconi A. Autocrine nerve growth factor in human keratinocytes. J Dermatol Sci. 2000;22(2):71-79. https://doi.org/10.1016/S0923-1811(99)00065-1
  26. Lim JH, Kang H, Park YM, Cho SH, Lee SY. Effect of substance P on Production of interleukin-12 and interferon-gamma from peripheral blood mononuclear cells of chronic severe atopic dermatitis patients. J Asthma. 2001;21(4):636-646.
  27. Lee IJ. Epidermal cytokine profiles in allergic and irritant contact dermatitis. Yonsei Univ Grad School, Doctoral dissertation. 2002; 28.
  28. Yu JS, Kim JU, Lee CH, Lee SR, Yook TH. The Effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice. J Korean Acupunct Moxibust Soc. 2015;32(4):119-131.
  29. Yu HS, Gim S, Song H, Ji J, Bal JW, Kim D. A Study on the Immune Modulation of Chunghwatang (CHT) in Atopic Dermatitis Animal Models. Daejeon J Institute Orient Med. 2012;21(1):53-64.

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