Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Prevalence of Major Methicillin-Resistant Staphylococcus aureus Clones in Korea Between 2001 and 2008

  • Kang, Gi Su (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Jung, Yung Hee (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Kim, Hwa Su (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Lee, Yeong Seon (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Park, Chan (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Lee, Kwang Jun (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC)) ;
  • Cha, Jeong Ok (Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC))
  • Received : 2016.01.14
  • Accepted : 2016.08.01
  • Published : 2016.11.01
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Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) are important pathogens causing nosocomial infections in Korean hospitals. This study aimed to investigate the epidemiological and genetic diversity of clinical S. aureus isolates in healthcare settings from 2001 to 2008. Methods: Samples and data were obtained from 986 individuals as part of the National Antimicrobial Surveillance Project, involving 10 regions nationwide. Molecular typing studies, including multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were performed, and a representative clone of Korean MRSA was classified by combinational grouping using a DiversiLab (DL; $bioM{\acute{e}}rieux$, France) repetitive element polymerase chain reaction (rep-PCR) system. Results: Nine Korean MRSA clones (KMRSA-1 to -9) were identified by analysis of genetic backgrounds and molecular characteristics. KMRSA-1 to -3, expressing clonal complex (CC) 5 (carrying SCCmec II), CC8 (carrying SCCmec III), and CC72 (carrying SCCmec IV) were spread nationwide. In contrast, KMRSA-6 was highly prevalent in Gyeongsangnamdo, and KMRSA-4 was highly prevalent in Jeollanam-do and Jeollabuk-do. Conclusions: Epidemic KMRSA clones were genetically similar to major clones identified from the USA, with the exception of KMRSA-2, which had the SCCmec III type. Our results provide important insights into the distribution and molecular genetics of MRSA strains in Korea and may aid in the monitoring of MRSA spread throughout the country.

Keywords

Acknowledgement

Supported by : Korea Centers for Disease Control and Prevention

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