Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Protective effect of STAR of STAR series on CCl4 induced acute hepatotoxicity by regulation of reactive oxygen species

활성산소종의 조절을 통한 음료 '별의별간'의 급성간독성 보호효과

  • Chang, Bo Yoon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Oh, Jun Seok (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Han, Ji Hye (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Kim, Da Eun (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Hong, Jae Heoi (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Kim, Sung Yeon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
  • 장보윤 (원광대학교 약학대학, 약품 연구소) ;
  • 오준석 (동부생약영농조합) ;
  • 한지혜 (원광대학교 약학대학, 약품 연구소) ;
  • 김다은 (원광대학교 약학대학, 약품 연구소) ;
  • 홍재희 (동부생약영농조합) ;
  • 김성연 (원광대학교 약학대학, 약품 연구소)
  • Received : 2016.02.29
  • Accepted : 2016.04.04
  • Published : 2016.04.30
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Abstract

STAR of STAR (SS 01-04) is a series of drinks that consist of various extracts obtained from Coriolus versicolor, Artemisia capillaris Thunb., Hovenia dulcis, Acanthopanax sessiliflorus, Lycium chinense, Citrus reticulata, Saururus chinensis, Pueraria lobata, Pyrus pyrifolia, and Oenanthe javanica. A purpose of this study was to investigate the hepatoprotective effect of SS 01-04. Antioxidant activity of the drinks was evaluated by conducting a hydroxyl radical-scavenging assay. Cytotoxicity and hepatoprotective potential were determined using HepG2 cells in vitro, while protective effects against acute hepatotoxicity was evaluated in vivo. The antioxidant activity of the SS 01-04 at concentration of 100 and 250 mg/mL was similar to that of $50{\mu}M$ vitamin C. tert-Butyl hydroperoxide (tBHP)-induced production of reactive oxygen species (ROS) was blocked by SS 01, 03 and 04 in a dose-dependent manner. Treatment with SS 04 significantly lowered the serum levels of alanine aminotransferase and aspartate aminotransferase in an animal model of carbon tetrachloride $(CCl_4)-induced$ hepatotoxicity (p<0.05). In addition, SS04 increased glutathione level while decreased malondialdehyde level in the liver considerably (p<0.05). It also inhibited the $CCl_4-induced$ increase in the levels of triglyceride and cholesterol in serum and the liver. These findings indicated that SS 01-04 possessed antioxidant activity and protect against ROS. In particular, SS 04 is potentially highly beneficial in treating liver damage as it scavenges reactive free radicals and boosts the endogenous antioxidant system.

본 연구는 4종의 별의별간(SS) 음료의 간보호 소재로서의 이용 가능성을 조사하고자 항산화능 평가, t-BHP 와 $CCl_4$로 산화적 손상 및 급성 간독성 유도한 in vitro, in vivo 모델을 활용하여 간보호능을 평가하였다. 실험결과, 별의별간 01~04는 $50{\mu}M$ vitamin C 와 유사한 항산화 효과를 나타내었다. HepG2 세포에 t-BHP로 산화 스트레스를 유도한 뒤 나타나는 세포독성에 대해 별의별간 01 및 04에서 농도 의존적인 세포 보호효과를 보였으며, ROS 생성 억제에서 별의별간 01, 03, 04에서 농도의존적인 억제를 나타내었다. 미나리가 혼합된 별의별간 04에 대한 급성 간손상 in vivo 모델을 활용하여 간보호능 검증 결과, 별의별간 04는 $CCl_4$로 증가된 혈중 ALT, AST의 유의적 감소, 간 조직중 증가된 MDA 함량 감소 및 감소된 GSH의 유의적 증가를 나타내었다. 또한, 혈청 및 간 조직에서 증가된 중성지방과 콜레스테롤을 유의적으로 감소시켰다. 이러한 결과를 종합하며, 별의별간 04는 in vitro 및 in vivo 모델에서 산화적 손상에 대해 간보호 효과를 나타내었다.

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