Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Columbianadin Inhibits Cell Proliferation by Inducing Apoptosis and Necroptosis in HCT116 Colon Cancer Cells

  • Kang, Ji In (College of Pharmacy, Seoul National University) ;
  • Hong, Ji-Young (College of Pharmacy, Seoul National University) ;
  • Choi, Jae Sue (Department of Food Science and Nutrition, Pukyong National University) ;
  • Lee, Sang Kook (College of Pharmacy, Seoul National University)
  • Received : 2015.09.08
  • Accepted : 2015.12.29
  • Published : 2016.05.01
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Abstract

Columbianadin (CBN), a natural coumarin from Angelica decursiva (Umbelliferae), is known to have various biological activities including anti-inflammatory and anti-cancer effects. In this study, the anti-proliferative mechanism of actions mediated by CBN was investigated in HCT-116 human colon cancer cells. CBN effectively suppressed the growth of colon cancer cells. Low concentration (up to $25{\mu}M$) of CBN induced apoptosis, and high concentration ($50{\mu}M$) of CBN induced necroptosis. The induction of apoptosis by CBN was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim and Bid, and the induction of necroptosis was related with RIP-3, and caspase-8. In addition, CBN induced the accumulation of ROS and imbalance in the intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase and GPx-1. These findings demonstrate that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.

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