Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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The Anti-Diabetic Effects and Nephroprotective Effect of Black Ginseng Prosapogenin Extract in Streptozotocin-Induced Mice

흑삼의 프로사포게닌 추출물이 Streptozotocin으로 유도된 당뇨 쥐에 대한 항당뇨 효과 및 신장보호 효과

  • Kong, Ryong (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Shon, Mi Yae (International Ginseng and Herb Research Institute) ;
  • Seo, Yun Soo (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok Hwa (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Zhou, Tian (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Do Yeon (International Ginseng and Herb Research Institute) ;
  • Choi, Sung Hoon (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kwon, Dong Yeul (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University)
  • 공룡 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 손미례 (금산국제인삼약초연구소) ;
  • 서윤수 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 주전 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 김도연 (금산국제인삼약초연구소) ;
  • 최성훈 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과 한약연구소)
  • Received : 2015.12.02
  • Accepted : 2016.02.13
  • Published : 2016.04.30
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Abstract

Background: This study examined the hypoglycemic and kidney protective effect of black ginseng in streptozotocin-induced diabetic mice. Methods and Results: Diabetes was induced by treating mice with streptozotocin (STZ) for four weeks. In vivo studies were performed in order to investigate the hypoglycemic effect of the black ginseng prosapogenin (GBG05-FF) extract. The body weight and blood glucose level were measured. Moreover, after the mice were sacrificed, the kidneys were isolated and histological changes were observed with hematoxylin and eosin staining. Blood urea nitrogen and creatinine levels were also measured. The results showed that administration of black ginseng increased body weight. Compared to blood glucose levels in STZ mice, blood glucose levels were reduced by 48% in STZ mice supplemented with 300 mg/kg of black ginseng, and by 69% in STZ mice supplemented with 900 mg/kg. Furthermore, histopathological examination of STZ mouse kidneys revealed, changes in the kidneys, epithelial cell damages, inflammatory cell infiltration and glomerulus hypertrophy. However, a significant reduction of glomerular water droplets (indicative of glomerulus hypertrophy) was observed in the kidneys of STZ mice supplemented with black ginseng extract. Conclusions: These results suggest that black prosapogenin (GBG05-FF) ginseng extract has a significant hypoglycemic effect and can be used as an anti-diabetic substance and renal protective agents as part of dietary supplements or novel drugs.

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References

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