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Content changes of Sulforaphane in Leaves of Brassica oleracea var. italica

브로콜리(Brassica oleracea var. italica) 잎에서 sulforaphane의 함량 변화

  • 정민철 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 임태헌 ((주)삼호유비 농생명과학연구소) ;
  • 고순보 (제주특별자치도 농업기술원) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부)
  • Received : 2016.02.11
  • Accepted : 2016.02.17
  • Published : 2016.02.29

Abstract

Analyzing the 13 sort contents of broccoli leaves by using GC/MS, sulforaphane was found in 11 sort of broccoli leaves for the result. After being grinded by the blinder, amount of sulforaphane in broccoli leaves was rapidly raised after thirty minutes and maintained the amount till sixty minutes have passed. Among the parts of broccoli, the root had the most sulforaphane. In freezing temperature, biosythesized sulforaphane maintained longer than in room temperature. However, even in frozen condition, the amount of sulforaphane was reduced to half or less after 3 weeks.

브로콜리 13종의 잎을 대상으로 sulforaphane의 함량을 분석한 결과, 2종에서는 검출되지 않았으나 11종에서는 검출되었다. "필그림" 품종이 가장 많은 sulforaphane 함량을 나타냈다. 브로콜리 잎을 blinder로 브로콜리의 잎을 균질화한 후에 sulforaphane의 생합성능은 30분 후에 급격히 증가하여 60분까지 함량이 유지되었다. 또한 sulforaphane은 동결건조를 할 경우 브로콜리 잎의 분말에서 검출되었으나 열풍건조를 할 경우 휘산 되어 분말에서 검출되지 않았다. 브로콜리의 부위별로(꽃 봉우리, 잎, 줄기 및 뿌리) sulforaphane의 함량을 분석한 결과, 뿌리에서 가장 많은 sulforaphane이 검출 되었고 다음으로 잎에서 높았다. 브로콜리 잎에서 생합성된 sulforaphane의 경시변화를 분석한 결과, 상온보관 보다 냉동보관 상태에서 오래 지속되었으나 냉동보관 조건에서도 3주 후에 sulforaphane의 함량이 1/2 이하로 감소하였다.

Keywords

References

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