Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지
DOI QR코드

DOI QR Code

Seed Disinfectant Effect of Pleurotus ostreatus (Heuktari) Extract on Fusarium fujikuroi Nirenberg

흑타리버섯 추출물의 벼 키다리병에 대한 종자소독 효과

  • 오태석 (공주대학교 식물자원학과) ;
  • 박윤진 (공주대학교 식물자원학과) ;
  • 김성민 (공주대학교 식물자원학과) ;
  • 신동일 (공주대학교 식물자원학과) ;
  • 김창호 (공주대학교 식물자원학과) ;
  • 조용구 (공주대학교 식물자원학과) ;
  • 장명준 (공주대학교 식물자원학과)
  • Received : 2016.02.12
  • Accepted : 2016.02.16
  • Published : 2016.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate antimicrobial activities of water extracted Pleurotus ostreatus var Heuktari (PO), and their application to F. fujikuroi Nirenberg growth inhibition material. Various organic solvents (chloroform, hexane, ethyl acetate, ethanol) were tested to investigate the antimicrobial activities of Pleurotus ostreatus var Heuktari against F. fujikuroi Nirenberg. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but butanol extract showed 2.5% strong activities in order of F. fujikuroi Nirenberg disk diffusion test. Then we observe Antifungal activity using green house. Bed soil and mushroom extract mixed at 10%, 7.5%, 5%, 2.5%, 1% total bed soil mass. Screening of Antifungal activity was tested two periode 18day and 25day. The Antifungal activity rate of each period and extract density was 7.5% mixed bedsoil has investigated 80% Antifungal activity rate. The result indicates that the Pleurotus ostreatus var Heuktari extract using F. fujikuroi Nirenberg registance, which shows the development of organic agriculture seed disinfectant.

본 연구에서는 흑타리버섯을 물, 에틸아세테이트, 클로로포름, 부탄올 등으로 추출하였다. 각 유기용매를 이용한 흑타리버섯 추출물의 항균 활성을 1차적으로 평판 확산방법을 사용하여 항균력을 검증하였다. 부탄올 추출물에서 가장 높은 항균력을 확인하였고, 1%, 2.5%, 5%, 7.5%, 10% 농도로 항균력을 확인하였을 때도 부탄올 추출물이 가장 높은 항균활성을 확인하였다. 위의 결과를 통해 실제 포장실험에서도 가능성을 확인하여 온실에서 F. fujikuroi Nirenberg의 억제력을 확인해본 결과 흑타리버섯 부탄올 추출물을 7.5% 첨가하였을 때 .80%의 높은 억제력을 확인할 수 있었다. 또한 초기의 발아력과 파종 후 30일 경과 후에 확인한 초기 생육특성에서도 흑타리버섯 추출물의 5% 이상에서는 방제에 효과가 높았으며 7.5% 이상 시에는 초장 및 묘소질이 대조구와 비교하여 통계적 유의차가 없는 높은 방제효과를 확인할 수 있었고 이러한 결과로 볼 때 흑타리버섯 추출물은 벼 키다리병에 대하여 높은 방제효과가 있으며 향후에는 친환경 종자소독제로써 활용가능성이 높을 것으로 판단된다.

Keywords

References

  1. Anke, T., H. J. Hecht, G. Schramm, and W. Steglich. 1979. Antibiotics from basidiomycetes. IX. Oudemansin, an antifungal antibiotic from Oudemansiella mucida (Schrader ex Fr.) Hoehnel (Agaricales). The Journal of Antibiotics. 32: 1112-1117. https://doi.org/10.7164/antibiotics.32.1112
  2. Anke, T., F. Oberwinkler, W. Steglich, and G. Hofle. 1977. The striatins-new antibiotics from the basidiomycete Cyathus striatus (Huds. ex Pers.) Willd. The Journal of Antibiotics. 30: 221-225. https://doi.org/10.7164/antibiotics.30.221
  3. Choi, M. R., H. J. Cho, J. S. Lee, H. Y. Kim, and T. S. Lee. 2011. The optimal culture conditions and antifungal activity of culture extract from Oudemansiella mucida. The Korean journal of Mycology. 39: 91-98. https://doi.org/10.4489/KJM.2010.39.2.091
  4. Han, K. Y., D. Yang, E. J. Chang, Y. Lee, H. Huang, S. H. Sung, Z. H. Lee, Y. C. Kim, and H. H. Kim. 2007. Inhibition of osteoclast differentiation and bone resorption by sauchinone. Biochemical Pharmacology. 74: 911- 923. https://doi.org/10.1016/j.bcp.2007.06.044
  5. Hemmi, T., F. Seto, and J. Ikeya. 1931. Studies on the 'bakanae' disease of the rice plant on the infection of rice by lisea fujikuroi sawada and gibberella saubinetii (mont.) Sacc. In the flowereing period. Forschn. Geb. pflankrankh, Kyoto. 1: 99-110.
  6. Kavanagh, F., A. Hervey, and W. J. Robbins. 1949. Antibiotic substances from basidiomycetes: IV. Marasmius conigenus. Proceedings of the National Academy of Sciences of the United States of America. 35: 343-349. https://doi.org/10.1073/pnas.35.7.343
  7. Kim, C. K. 1981. Ecological studies of bakanae disease of rice, caused by Gibberella fujikuroi. Korean Journal of Applied Entomology. 20: 146-150.
  8. Kim, H. J., M, S. Ahn., G. H. Kim, and M. H. Kang. 2006. Antioxidative and antimicrobial activities of Pleurotus eryngii extracts prepared from different aerial part. Korean journal of Food Science and Technology. 38: 799-804.
  9. Kim, J. B. 2005. Pathogen, insect and weed control effects of secondary metabolites from plants. Journal of the Korean Society for Applied Biological Chemistry. 48: 1-15.
  10. Kim, J. S. 2003. Development of biopesticide using higher plant-derived natural products. Korea Research Institute of Chemical Technology. 1: 75-97.
  11. Min, J. Y., E. M. Kim, and T. J. Min. 1997. Development of antibiotics in mushroom -The screening of antifungal activities in basidiomycetes-. The Korean journal of Mycology. 25: 354-361.
  12. Lee, C. J., J. C. Cheong, C. S. Jhune, and S. H. Kim. 2009. Applicability of spent mushroom media as horticultural nursery media. Korean J Of Soil Sciences and Fertilizer. 42: 117-122.
  13. Nirenberg, H. I. 1976. Untersuchungen uber die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt fur Land- Forstwirtschft Berlin- Dahlem. 169: 1-117.
  14. Ou, S. H. 1985. Rice Diseases. Kew, UK: Commonwealth Mycological Institute. pp. 117-280.
  15. Myung, I. S. K. S. Park, S. K. Hong, J. W. Park, H. S. Shim, Y. K. Lee, S. Y. Lee, S. D. Lee, S. H. Lee, H. S. Choe, H. W. Choi, S. G. Heu, D. B. Shin, D. S. Ra, W. H. Yeh, and W. D. Cho. 2005. Review of disease incidence of major crops of the South Korea in 2004. Research in Plant Disease. 11: 89-92. https://doi.org/10.5423/RPD.2005.11.2.089
  16. Park, W. S., W. H. Yeh, S. W. Lee, S. S. Han, J. S. Lee, C. K. Lim, and Y. H. Lee. 2008. Electron microscopic study for the influence of soaking in hot water and prochloraz solution on spore and mycelium of Fusarium fujikuroi infected in rice seed. Research in Plant Disease. 14: 176-181. https://doi.org/10.5423/RPD.2008.14.3.176
  17. Strobel, R. G., H. Quinn, and W. Lange. 1967. Grouth of Fusarium Diversisporum Sherb. On Long-Chain n-Fatty Alcohols or Cholesterol as The Sole Carbon Source. Canadian Journal of Microbiology, 13: 121-136. https://doi.org/10.1139/m67-018
  18. Sun, S. K. 1975. The disease cycle of rice bakanae disease in Taiwan. Proc. Natl. Sci. Counc. Repub. China. 8: 245-255.
  19. Takeuchi, T., H. Iinuma,, J. Iwanaga, S. Takahashi, T. Takita, and H. Umezawa. 1969. Coriolin, a new basidiomycetes antibiotic. The Journal of Antibiotics. 22: 215-217. https://doi.org/10.7164/antibiotics.22.215
  20. Umezawa, H., T. Takeuchi, H. Iinuma, M. Ito, M. Ishizuka, M. Ishizuka, Y. Kurakata, Y. Umeda, Y. Nakanishi, T. Nakamura, A. Obayashi, and O. Tanabe. 1975. A new antibiotic, calvatic acid. The Journal of Antibiotics. 28: 87-90. https://doi.org/10.7164/antibiotics.28.87