Journal of Plant Biotechnology

  • 제43권1호
  • /
  • Pages.119-124
  • /
  • 2016
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Influences of different light sources and light/dark cycles on anthocyanin accumulation and plant growth in Petunia

  • Ai, Trinh Ngoc (Department of Horticultural Science, Kyungpook National University) ;
  • Naing, Aung Htay (Department of Horticultural Science, Kyungpook National University) ;
  • Kim, Chang Kil (Department of Horticultural Science, Kyungpook National University)
  • 투고 : 2016.02.22
  • 심사 : 2016.03.04
  • 발행 : 2016.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Anthocyanin accumulation and plant growth were examined in petunia (NT and $T_2$ transgenic plants) by determining the effects of different sources of light and varying light/dark cycles. Red light significantly enhanced anthocyanin content of B-peru+mPAP1; however, it had a negative effect on anthocyanin production in RsMYB1 plants. In general, white light was found to be reasonable for anthocyanin accumulation in all plants. In case of light/dark cycles, application of seven days of light:14 days of dark significantly enhanced anthocyanin content. We found that anthocyanin content detected in transgenic plants expressing anthocyanin regulatory transcription factor genes (B-peru+mPAP1 or RsMYB1) was higher than that in NT plants in all treatments. Plant growth was also influenced by the different light sources and dark/light cycles. Taken together, our results suggest that light source and light/dark cycle play an important role in anthocyanin production and plant growth. The choice of the optimal conditions is also important for anthocyanin production and plant growth depending on NT or transgenic plants carrying anthocyanin regulatory transcription factors.

키워드

참고문헌

  1. Ai TN, Arun M, Naing AH, Lim SH, Kim CK (2016) The combinatorial expression of transcription factor genes B-Peru and mPAP1 enhances anthocyanin accumulation in transgenic Petunia hybrid. Scientia Horticulturae 10.1016/j.scienta.2016.01.006
  2. Akoyunoglou G, Anni H (1984) Blue light on chloroplast development in higher plants. In Senger, H. (ed.), Blue Light in Biological Systems. Springer-Verlag, Berlin, West Germany. pp. 397-406
  3. Azad MOK, Chun IJ, Jeong JH, Kwon ST, Hwang JM (2011) Response of the growth characteristics and phytochemical contents of pepper (Capsicum annuum L.) seedlings with supplemental LED light in glass house. Bio-Environment Cont. 20:182-188
  4. Biran I, Halevy AH (1974) Effects of varying light intensity and temperature treatments applied to whole plants, or locally to leaves or flower buds, on growth and pigmentation of 'Baccara' Roses. Physiol. Plant 31:175-179 https://doi.org/10.1111/j.1399-3054.1974.tb03686.x
  5. Bowler C, Neuhaus G, Yamagata H, Chua NH (1994) Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77:73-81 https://doi.org/10.1016/0092-8674(94)90236-4
  6. Chan LK, Koay SS, Boey PL, Shatt A (2010) Effects of abiotic stress on biomass and anthocyanin production in cell cultures of Melastoma malabathricum. Biol Res. 43:127-135
  7. Chen DQ, Li ZY, Pan RC, Wang XJ (2006) Anthocyanin accumulation mediated by blue light and cytokinin in Arabidopsis seedlings. J. Integr. Plant Biol. 48:420-425 https://doi.org/10.1111/j.1744-7909.2006.00234.x
  8. Devlin PF, Rood SB, Somers DE, Quail PH, Whitelam GC (1992) Photophysiology of the elongated intemode (ein) mutant of Brassica rapa. Plant Physiol. 100:1442-1447 https://doi.org/10.1104/pp.100.3.1442
  9. Dong YH, Beuning L, Davies K, Mitra D, Morris B, Kootstra A (1998) Expression of pigmentation genes and photo-regulation of anthocyanin biosynthesis in developing Royal Gala apple flowers. Austr. J. Plant Physiol. 25:245-252 https://doi.org/10.1071/PP97108
  10. Feng S, Wang Y, Yang S, Xu Y, Chen X (2010) Anthocyaninbiosynthesis in pearsis regulated by a R2R3-MYB transcription factor PyMYB10. Planta 232:245-255 https://doi.org/10.1007/s00425-010-1170-5
  11. How FW, Smith MA (2003) Effect of light/dark cycling on growth and anthocyanin production of Ajuga reptens in callus culture. J. Agric. Res. China 52:291-296
  12. Kadomura IY, Miyawaki K, Noji S, Takahashi A (2013) Phototropin2 is involved in blue light-induced anthocyanin accumulation in Fragaria $\times$ ananassa fruits. J. PlantRes. 126:847-857
  13. Konczak II, Yoshinaga M, Nakatani M, Terahara N, Yamakawa O (2000) Establishment and characteristics of an anthocyaninproducing cell line from sweet potato storage root. Plant Cell Rep 19:472-477 https://doi.org/10.1007/s002990050758
  14. Lee JG, Oh SS, Cha SH, Jang YA, Kim SY, Um YC, Cheong SR (2010) Effects of red/blue light ratio and short-term light quality conversion on growth and anthocyanin contents of baby leaf lettuce. Bio-Environment Cont. 19:351-359 (in Korean)
  15. Lim SH, Sohn SH, Kim DH, Kim JK, Lee JY, Kim YM, Ha SH (2012) Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant. Plant Biotechnol Rep 6:203-211 https://doi.org/10.1007/s11816-012-0215-6
  16. Lim SH, Song JH, Kim DH, Kim JK, Lee JY, Kim YM, Ha SH (2015) Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1. Plant Cell Reports doi:10.1007/s00299-015-1909-3
  17. Martin C, Prescott A, Mackay S, Bartlett J, Vrijlandt E (1991) Control of anthocyanin biosynthesis in flowers of Antirrhium majus. Plant J. 1:37-49 https://doi.org/10.1111/j.1365-313X.1991.00037.x
  18. Moscovici S, Moalem BD, Weiss D (1996) Leaf mediated light responses in petunia flowers. Plant Physiol. 110:1275-1282 https://doi.org/10.1104/pp.110.4.1275
  19. Naing AH, Lim KB, Kim CK (2015) The usage of snapdragon Delila (Del) gene as a visible selection marker for the antibiotic-free transformation system. J Plant Biol 58:110-6 https://doi.org/10.1007/s12374-014-0423-4
  20. Nakamura M, Takeuchi Y, Miyanaga K, Seki M, Furusaki S (1999) High anthocyanin accumulation in the dark by strawberry (Fragaria ananassa) callus. Biotechnology letters 21:695-699 https://doi.org/10.1023/A:1005558325058
  21. Sakamoto K, Lida K, Sawamura K, Hajiro K, Asada Y, Yoshikawa T, Furuya T (1993) Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata. Phytochemistry 33:357-360 https://doi.org/10.1016/0031-9422(93)85517-U
  22. Senger H (1982) The effect of blue light on plants and microorganisms. Photochemistry and Photobiol. 35:911-920 https://doi.org/10.1111/j.1751-1097.1982.tb02668.x
  23. Zeiger E (1984) Blue light and stomatal function. In Senger, H. (ed.), Blue Light in Biological Systems. Springer-Verlag, Berlin, West Germany. pp. 484-494