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Isoproterenol과 Melatonin의 첨가가 돼지 단위발생란의 체외발달에 미치는 영향

Effects of Isoproterenol and Melatonin Supplementation on In Vitro Development of Parthenogenetic Activated Oocytes in Pig

  • 정다운 (경남과학기술대학교 동물생명과학과) ;
  • 윤윤진 (경남과학기술대학교 동물생명과학과) ;
  • 박희성 (경남과학기술대학교 동물생명과학과)
  • Jeong, Da-Un (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Yun, Yun-Jin (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Park, Hee-Sung (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
  • 투고 : 2016.02.15
  • 심사 : 2016.03.01
  • 발행 : 2016.03.31

초록

In this study, to improve the in vitro development of various cells including cloned embryos, the effects that isoproterenol and melatonin have on in vitro development of porcine parthenogenetic oocytes were investigated. Parthenogenetic activation was induced with electrical stimulation, BSA and 6-DMAP treatment. $10^{-7}M$ of melatonin and isoproterenol ($10^{-10}$, $10^{-12}$ and $10^{-14}M$) were supplemented for in vitro maturation (IVM) and in vitro culture (IVC) medium, with different concentrations. When isoproterenol and melatonin were supplemented in IVM medium with different concentrations, there was no significant (P<0.05) difference of maturation rate in the treatment groups as well as in that of only melatonin. As isoproterenol and melatonin were supplemented in IVM medium with different concentrations, blastocyst rates of isoproterenol $10^{-12}M$ treatment group (37.1%) were significantly (P<0.05) higher than control group (26.0%). Isoproterenol and melatonin were supplemented in IVC medium with different concentrations, then the cleavage rate of $10^{-12}M$ isoproterenol treatment group (82.2%) was significantly (P<0.05) higher than the group that melatonin was only supplemented (70.9%). There was no difference of blastocyst rate between the treatment groups. When isoproterenol and melatonin were supplemented for IVM+IVC medium with different concentrations, the cleavage rate of $10^{-12}M$ isoproterenol treatment group (92.5%) was significantly (P<0.05) higher than the control group (82.8%) and the group that melatonin was only treated (81.6%). The blastocyst rate of $10^{-12}M$ as 45.6% was significantly (P<0.05) higher than control group (25.2%) and melatonin treatment group (31.2%). The cell number of blastocyst in $10^{-12}M$ isoproterenol treatment group $35.5{\pm}3.4$ was significantly (P<0.05) highest. The results of this study showed that the development rate of IVC when both isoproterenol and melatonin were supplemented was higher than when melatonin was only supplemented. Therefore, it is concluded that isoproterenol is rather effective in the activation of melatonin. $10^{-7}M$ melatonin and $10^{-12}M$ isoproterenol were considered suitable concentration.

키워드

참고문헌

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