Asian Pacific Journal of Cancer Prevention

  • 제17권3호
  • /
  • Pages.1163-1168
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  • 2016
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Plasma Peptidome as a Source of Biomarkers for Diagnosis of Cholangiocarcinoma

  • Kotawong, Kanawut (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Thitapakorn, Veerachai (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Roytrakul, Sittiruk (Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thammasat University) ;
  • Phaonakrop, Narumon (Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thammasat University) ;
  • Viyanant, Vithoon (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Na-Bangchang, Kesara (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine)
  • 발행 : 2016.04.11
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초록

Cholangiocarcinoma (CCA) is the bile duct cancer which constitutes one of the important public health problems in Thailand with high mortality rate, especially in the Opisthorchis viverrini (a parasite risk factor for CCA) endemic area of the northeastern region of the country. This study aimed to identify potential biomarkers from the plasma peptidome by CCA patients. Peptides were isolated using 10 kDa cut-off filter column and the flow-through was then used as a peptidome for LC-MS/MS analysis. A total of 209 peptides were obtained. Among these, 15 peptides were concerned with signaling pathways and 12 related to metabolic, regulatory, and biosynthesis of secondary metabolite pathways. Five exclusive peptides were identified as potential biomarkers, i.e. ETS domain-containing transcription factor ERF (P50548), KIAA0220 (Q92617), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1 (P42338), LP2209 (Q6XYC0), and casein kinase II subunit alpha (P19784). Three of these biomarkers are signaling related molecules. A combination of these biomarkers for CCA diagnosis is proposed.

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참고문헌

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피인용 문헌

  1. CK2 in Cancer: Cellular and Biochemical Mechanisms and Potential Therapeutic Target vol.10, pp.1, 2017, https://doi.org/10.3390/ph10010018