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Principal Component Analysis and Molecular Characterization of Reniform Nematode Populations in Alabama

  • Nyaku, Seloame T. (Department of Crop Science, College of Basic and Applied Sciences, University of Ghana) ;
  • Kantety, Ramesh V. (Department of Natural Resources and Environmental Sciences, Alabama A & M University) ;
  • Cebert, Ernst (Department of Natural Resources and Environmental Sciences, Alabama A & M University) ;
  • Lawrence, Kathy S. (Department of Entomology and Plant Pathology, Auburn University) ;
  • Honger, Joseph O. (Soil and Irrigation Research Centre, University of Ghana) ;
  • Sharma, Govind C. (Department of Natural Resources and Environmental Sciences, Alabama A & M University)
  • 투고 : 2015.09.15
  • 심사 : 2015.11.10
  • 발행 : 2016.04.01

초록

U.S. cotton production is suffering from the yield loss caused by the reniform nematode (RN), Rotylenchulus reniformis. Management of this devastating pest is of utmost importance because, no upland cotton cultivar exhibits adequate resistance to RN. Nine populations of RN from distinct regions in Alabama and one population from Mississippi were studied and thirteen morphometric features were measured on 20 male and 20 female nematodes from each population. Highly correlated variables (positive) in female and male RN morphometric parameters were observed for body length (L) and distance of vulva from the lip region (V) (r = 0.7) and tail length (TL) and c' (r = 0.8), respectively. The first and second principal components for the female and male populations showed distinct clustering into three groups. These results show pattern of sub-groups within the RN populations in Alabama. A one-way ANOVA on female and male RN populations showed significant differences ($p{\leq}0.05$) among the variables. Multiple sequence alignment (MSA) of 18S rRNA sequences (421) showed lengths of 653 bp. Sites within the aligned sequences were conserved (53%), parsimony-informative (17%), singletons (28%), and indels (2%), respectively. Neighbor-Joining analysis showed intra and inter-nematodal variations within the populations as clone sequences from different nematodes irrespective of the sex of nematode isolate clustered together. Morphologically, the three groups (I, II and III) could not be distinctly associated with the molecular data from the 18S rRNA sequences. The three groups may be identified as being non-geographically contiguous.

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참고문헌

  1. Agudelo, P., Robbins, R. T., Stewart, J. M. and Szalanski, A. L. 2005. Intra-specific variability of Rotylenchulus reniformis from cotton-growing regions in the United States. J. Nematol. 37:105-114.
  2. Carranza, S., Giribet, G., Ribera, C., Baguna, J. and Riutort, M.1996. Evidence that two types of 18S rDNA coexist in the genome of Dugesia (Schmidtea) mediterranea (Platyhelminthes, Turbellaria, Tricladida). Mol. Biol. Evol. 13:824-832. https://doi.org/10.1093/oxfordjournals.molbev.a025643
  3. Carter, L., McLean, K. S. and Lawrence, G. W. 2002. Recovery and viability of Rotylenchulus reniformis from naturally infested field soil placed in controlled cold storage. Proc. Beltwide Cotton Conf., Atlanta, GA 8-12 Jan. 2002. Natl. Cotton Counc. Am., Memphis TN.
  4. Counc. Am., Memphis TN. Cook, R. and Evans, K. 1987. Resistance and tolerance. In: Principles and practice of nematode control in crops, eds, by R.H. Brown and B.R. Kerry. pp. 179-232. Acad. Press, Sydney.
  5. Dasgupta, D. R., Raski, D. J. and Sher, S. A. 1968. A revision of the genus Rotylenchlus Linford and Oliveira, 1940 (Nematoda: Tylenchidae). Proc. Helminth. So. of Washington 35:169-172.
  6. Deng, D., Zipf, A., Tilahun, Y., Sharma, G. C., Jenkins, J. and Lawrence, K. 2008. An improved method for the extraction of nematodes using iodixanol (OptiPrepTM). Afr. J. Microbiol. Res. 2:167-170.
  7. Eickbush, T. H. and Eickbush, D. G. 2007. Finely orchestrated movements: Evolution of the ribosomal RNA genes. Genetics 175:477-485. https://doi.org/10.1534/genetics.107.071399
  8. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  9. Fitch, D. H. A., Bugaj-Gaweda, B. and Emmons, S. W. 1995. 18S ribosomal rRNA gene phylogeny for some Rhabditidae related to Caenorhabditis. Mol. Biol. Evol. 12:346-358.
  10. Gazaway, W. S., Akridge, J. R. and McLean, K. S. 2001. Impact of nematicides on cotton production in reniform infested fields. p. 128. Proc. Beltwide Cotton Conf., Anaheim, CA.
  11. Hillis, D. M. and Dixon, M. T. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol. 66:411-453. https://doi.org/10.1086/417338
  12. Jianghua, H., Zeng, Y. and Chen, X. 2013. Morphological, enzymatic and molecular characterization of Meloidogyne arenaria from Anubias barteri var. caladiitolia in China. Nematropica 43:261-270.
  13. Kirkpatrick, T. L. and Robbins, R. T. 1998. Nematodes of Cotton in Arkansas. Cooperative Extension Service, University of Arkansas Fact Sheet FSA 7523-3M-4-98RV.
  14. Koenning, S. R., Kirkpatrick, T. L., Starr, J. L., Wrather, J. A., Walker, N. R. and Mueller, J. D. 2004. Plant-parasitic nematodes attacking cotton in the United States: Old and emerging production challenges. Plant Dis. 88:100-113. https://doi.org/10.1094/PDIS.2004.88.2.100
  15. Lawrence, G. W. and McLean, K. S.1999. Managing the reniform nematode with nematicides. Proc. Beltwide Cotton Prod. Res. Conf., Orlando, FL, pp. 100-101.
  16. Linford, M. B. and Oliveira, J. M. 1940. Rotylenchulus reniformis, nov. gen., n. sp., nematode parasite of roots. Proc. Helminth. Soc. Wash. 7:35-42.
  17. Nadler, S. A. and Hudspeth, D. S. S. 1998. Ribosomal DNA and phylogeny of the Ascaridoidea (Nemata: Secernentea): Implications for morphological evolution and classification. Mol. Phylogenet. Evol. 10:221-236. https://doi.org/10.1006/mpev.1998.0514
  18. Nakasono, K. 1983. Studies on morphological and physio-ecological variations on the reniform nematode, Rotylenchulus reniformis Linford and Oliveira, 1940 with an emphasis on different geographical distribution of amphimictic and pathenogenetic populations in Japan. Bulletin of the Natl. Inst. Agric. Sci., Japan C 38:1-67.
  19. Nyaku, S. T., Kantety, R. V., Lawrence, K. S., van Santen, E. and Sharma, G. C. 2013. Canonical discriminant analysis of Rotylenchulus reniformis in Alabama. Nematropica 43:171-181. http://journals.fcla.edu/nematropica/article/view/82705
  20. Nyaku, S. T., Kantety, R. V., Tilahun, Y., Lawrence, K. S., Soliman, K. M., Cebert, E. and Sharma, G. C. 2013. 18S and ITS1 Genomic sequence variations in Rotylenchulus reniformis isolates in Alabama. J. Cotton Sci. 17:184-194. http://www.cotton.org/journal/2013-17/3/184.cfm
  21. Nyaku, S. T., Sripathi,V. R., Kantety, R. V., Gu, Y. Q., Lawrence, K. and Sharma, G. C. 2013. Characterization of the two intra-individual sequence variants in the 18S rRNA gene in the plant parasitic nematode, Rotylenchulus reniformis. PLoS ONE 8: 1-13.
  22. Overstreet, C. 1999. Reniform nematode - An introduction. Proc. Beltwide Cotton Conf., Orlando, FL, pp. 100.
  23. Powers, T. O., Mullin, P. G., Harris, T. S., Sutton, L. A. and Higgins, R. S. 2005. Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey. J. Nematol. 37:226-235.
  24. Robbins, R. T. 1994. Variation in the size of Rotylenchulus reniformis eggs, J2 males, and young females from resistant soybean. J. Nematol. 26:109-110.
  25. Robinson, A. F. 1999. Resistance to reniform nematode in upland cotton. Proc. Beltwide Cotton Conf. Memphis, TN. pp. 101.
  26. Robinson, A. F., Inserra, R. N., Caswell-Chen, E. P., Vovlas, N. and Troccoli, A. 1997. Rotylenchulus species: Identification, distribution, host ranges, and crop plant resistance. Nematropica 27:127-180.
  27. Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  28. Sivakumar, C. V. and Seshadri, A. R. 1971. Life history of the reniform nematode Rotylenchulus reniformis Linford and Oliveira, 1940. Indian J. Nematol. 1:7-20.
  29. Soares, P. L. M., dos Santos, J. M. and Lehman, P. S. 2003. Estudo morfométrico comparative de populações de Rotylenchulus reniformis (Nemata: Rotylenchulinae) do Brasil. Fitopatologia Brasileira 28:292-297. https://doi.org/10.1590/S0100-41582003000300011
  30. Tamura, K., Nei, M. and Kumar, S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. USA 101:11030-11035. https://doi.org/10.1073/pnas.0404206101
  31. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729. https://doi.org/10.1093/molbev/mst197
  32. Tilahun, Y., Soliman, K., Lawerence, K. S., Cseke, L. J. and Ochieng, J. W. 2008. Nuclear ribosomal DNA diversity of a cotton pest (Rotylenchulus reniformis) in the United States. Afri. J. Biotech. 7:3217-3224.
  33. Ye, W. M. and Robbins, R. T. 2004. Stepwise and canonical discriminant analysis of Longidorus species (Nematoda: Longidoridae) from Arkansas. J. Nematol. 36:449-457.