Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Phylogenetic Analyses of Pepper mild mottle virus and Cucumber mosaic virus Isolated from Rorippa palustris

속속이풀에서 분리한 고추마일드모틀바이러스와 오이모자이크바이러스의 계통발생학적 특성

  • Kwon, Sun-Jung (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Yoon, Ju-Yeon (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug-Seoun (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 권선정 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 윤주연 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최승국 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2015.07.27
  • Accepted : 2016.01.08
  • Published : 2016.03.31
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Abstract

During a field survey in 2014, a Rorippa palustris plant showing virus-like symptom was collected from a pepper field in Dangjin in Korea. The collected sample was subjected to examine infection with pepper-infecting viruses. Molecular diagnosis assay showed that the collected R. palustris sample was co-infected with Pepper mild mottle virus (PMMoV) and Cucumber mosaic virus (CMV). This is the first identification of PMMoV from R. palustris and the first report of CMV infection of R. palustris in Korea. To examine phylogenetic positions of the identified PMMoV and CMV isolates, their complete genome sequences of were determined and compared with those of previously reported isolates of the cognate viruses. Phylogenetic analyses revealed the isolates of PMMoV and CMV obtained from R. palustris are closely related to the pepper isolates of the cognate viruses. Our results suggest that R. palustris could act a weed reservoir of PMMoV and CMV.

2014년도 충남 당진의 고추포장 바이러스 발생 조사 중, 포장 주변에서 바이러스 병징을 보이는 속속이풀을 수집하였다. 수집한 시료에 대해 여러 고추감염바이러스의 감염여부를 조사한 결과, PMMoV와 CMV에 복합감염되어 있음을 확인하였다. 이는 PMMoV의 잡초 기주로서 속속이풀의 최초 동정이며, 속속이풀의 CMV 감염의 국내 첫 보고이다. 따라서 이들 신규 분리주들의 계통발생학적 위치를 파악하고자, 이들의 전체 게놈 염기서열을 분석하였고 이미 보고된 PMMoV 및 CMV 분리주의 유전정보와 비교 분석하였다. 유전적 측면에서 이미 보고된 고추감염바이러스와의 관계를 알아보고자 속속이풀에서 분리된 PMMoV와 CMV의 전체 염기서열을 분석하였고, 이들의 계통발생학적 특성을 구명하였다. 그 결과, 속속이풀에서 분리한 PMMoV 및 CMV의 분리주가 고추에서 분리한 분리주들과 매우 높은 유사성을 보임을 확인하였고, 이는 속속이풀이 PMMoV 및 CMV의 중간 기주로 작용할 수 있음을 시사한다.

Keywords

References

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