Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effects of Dietary DFA IV on Lumen Short Chain Fatty Acids Production and Intestinal Environment in Rats

식이 DFA IV의 흰쥐 장내 단쇄지방산 생성 및 장내환경 개선효과

  • Hong, Kyung Hee (Dept. of Food Science and Nutrition, Dongseo University) ;
  • Jang, Ki-Hyo (Dept. of Food and Nutrition, Kangwon National University) ;
  • Kang, Soon Ah (Institute of Health Industry, Hoseo University/Dept. of Conversing Technology, Graduate School of Venture, Hoseo University)
  • 홍경희 (동서대학교 식품영양학과) ;
  • 장기효 (강원대학교 식품영양학과) ;
  • 강순아 (호서대학교 보건산업연구소/호서대학교 벤처대학원 융합공학과)
  • 투고 : 2015.11.16
  • 심사 : 2016.03.03
  • 발행 : 2016.03.31
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초록

This study examined the improving effect of DFA IV obtained from bacterial fermentation on the gut health. The effects of the dietary DFA IV on the intestinal mass, short chain fatty acids production and pH were evaluated in vivo. Sprague-Dawley rats were fed the 0% (control) or 1% DFA IV supplemented diets for 3 weeks. Supplementation of DFA IV resulted in a significant increase in cecal tissue and wall weights. Together with the lowering of the cecal and colonic pH, the amount of acetate and butyrate increased by 1.6 and 3.2 fold of the control group in the cecum, respectively, in the rats fed DFA IV diets. The DFA IV diet also significantly increased the cecal lactate 1.5 fold compared to the control diet, indicating that dietary DFA IV stimulated the growth of lactic acid bacteria and bifidobacteria in the intestine. Based on the above results, it is concluded that the dietary DFA IV may be used as a putative prebiotic supplement.

본 연구는 미생물 발효로 생산된 DFA IV의 장내환경 개선 효과를 살펴보고자 하였다. 동물실험을 통해 식이 DFA IV가 장의 길이 및 무게와 단쇄지방산 및 젖산 생성, 장내 pH에 미치는 영향을 조사하였다. Sprague-Dawley종 흰쥐를 식이무게의 DFA IV를 0%(대조군) 또는 1%로 함유한 식이로 3주간 사육하였다. DFA IV를 공급한 군에서 대조군에 비해 맹장의 무게와 맹장벽의 무게가 유의적으로 증가하였다. DFA IV 공급에 의해 맹장 내용물의 아세트산과 부티르산 함량이 각각 대조군의 1.6배, 3.2배 증가하였고, 이에 따라 맹장과 대장의 pH도 DFA군에서 대조군에 비해 감소하였다. DFA IV는 또한 맹장의 젖산 생성을 1.5배 증가시켜, 식이로 공급한 DFA IV가 장내에서 젖산균의 증식을 촉진하였음을 보여주었다. 본 연구 결과 DFA IV 함유 식이는 장에서 발효되어 장내 환경을 개선하는 것으로 사료된다. 결론적으로 DFA IV는 장건강에 유익한 프리바이오틱 기능성 소재로 활용될 수 있으리라 기대된다.

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