KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Evaluation of Maize Downy Mildew using Spreader Row Technique

Spreader Row Technique을 이용한 옥수수 노균병 검정

  • Kim, Kyung-Hee (Department of Life Science, Dongguk University-Seoul) ;
  • Moon, Jun-Cheol (Agriculture and Life Sciences Research Institute, Kangwon National University) ;
  • Kim, Jae Yoon (College of Life Science and Biotechnology, Korea University) ;
  • Kim, Hyo Chul (Department of Life Science, Dongguk University-Seoul) ;
  • Shin, Seungho (Department of Life Science, Dongguk University-Seoul) ;
  • Song, Kitae (Department of Life Science, Dongguk University-Seoul) ;
  • Baek, Seong-Bum (Upland Crop Research Division, NICS, RDA) ;
  • Lee, Byung-Moo (Department of Life Science, Dongguk University-Seoul)
  • 김경희 (동국대학교 생명과학과) ;
  • 문준철 (강원대학교 농업생명과학연구원) ;
  • 김재윤 (고려대학교 생명과학대학 생명공학부) ;
  • 김효철 (동국대학교 생명과학과) ;
  • 신승호 (동국대학교 생명과학과) ;
  • 송기태 (동국대학교 생명과학과) ;
  • 백성범 (국립식량과학원 전작과) ;
  • 이병무 (동국대학교 생명과학과)
  • Received : 2015.11.17
  • Accepted : 2016.02.02
  • Published : 2016.03.31
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Abstract

This study was conducted to evaluate maize downy mildew resistance using spreader row technique in Cambodia. A total of forty maize lines were used in this experiment. Seven Korean varieties and seven breeding lines showed high infection rates (80~100%) and highly susceptible (HS) to downy mildew disease in both spring and fall. Also most of nested association mapping (NAM) parent lines were highly susceptible (HS). Meanwhile three inbred lines, Ki3, Ki11, and CML228, showed highly resistant (HR) or resistant (R) in spring and moderately resistant (MR) in fall. These three lines were already known as resistant inbred lines against downy mildew disease. It appears that spreader row technique was suitable for selection of maize downy mildew resistance in Cambodia. The incidence of downy mildew was influenced by weather conditions, especially relative humidity and temperature. Among several inoculation methods to screen for downy mildew resistance, this spreader row technique is effectively and easily used in the field of Southeast Asia.

옥수수 노균병에 대한 저항성 및 감수성을 확인하기 위하여 캄보디아 현지에서 노균병을 수집 및 채집하였으며, spreader row technique을 이용하여 봄/여름과 늦여름/가을동안 시험포장에 노균병을 접종한 후 4주차와 6주차에 노균병 발병률을 확인하였다. 국내 품종 7개와 육성 품종 7개는 노균병 접종 후 6주차에서 노균병에 대하여 대부분 highly susceptible (HS)의 등급을 확인하였으며, 발병률은 80~100%로 NAM parent계통보다 높게 나타났다. 이것은 국내 품종과 육성 품종 모두는 노균병에 대한 감수성이 있는 품종으로 확인하였다. 반면, Ki3, Ki11, CML228 계통은 봄/여름 시즌에서는 0~5%의 발병률과 highly resistant (HR) 또는 resistant (R)의 등급을 확인할 수 있었고, 늦여름/가을 시즌에는 22.2~25%의 발병률 및 moderately resistant (MR)의 발병 등급이 나타났다. 효율적인 노균병 접종을 위해서 기상 조건 및 습도, 그리고 온도를 중요시하며, 접종 방법 또한 간단하면서 손쉽게 관리할 수 있는 방법이 가장 중요하다. 본 연구에서 사용한 spreader row technique은 다양한 병징 연구에 활용이 가능하며, 저항성 및 감수성 품종을 효율적으로 확인 및 선발할 수 있다.

Keywords

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