미생물학회지 (Korean Journal of Microbiology)

  • 제52권1호
  • /
  • Pages.32-39
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  • 2016
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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독도 번행초에서 분리된 내생균류의 배양적 특성과 Aspergillus tubingensis YH103의 gibberellin A7의 생산

Gibberellin A7 production by Aspergillus tubingensis YH103 and cultural characteristics of endophytic fungi isolated from Tetragonia tetragonoides in Dokdo islands

  • 유영현 (국립해양생물자원관 해양미생물팀) ;
  • 박종명 (경북대학교 생명과학부) ;
  • 임성환 (국립낙동강생물자원관 담수생물다양성연구실) ;
  • 강상모 (경북대학교 응용생명과학부) ;
  • 박종한 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 이인중 (경북대학교 응용생명과학부) ;
  • 김종국 (경북대학교 생명과학부)
  • You, Young-Hyun (Marine Microorganism Team, National Marine Biodiversity Institute of Korea) ;
  • Park, Jong Myong (School of Life Science, Kyungpook National University) ;
  • Lim, Sung Hwan (Freshwater Biodiversity Research Division, Nakdonggang National Institute of Biological Resources) ;
  • Kang, Sang-Mo (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Jong-Han (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 투고 : 2015.12.28
  • 심사 : 2016.02.03
  • 발행 : 2016.03.31
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초록

독도에 자생하는 번행초의 뿌리로부터 순수 분리하여 형태적으로 상이한 17개의 내생균류를 선별하였다. 또한 분리된 균류들에 대하여 각각의 염농도와 pH 농도 구배에 따라 생장 시험을 확인하였다. 내생균류에 대해 각각 난장이벼의 유묘에 식물생장활성시험을 진행하였고, 그 결과 YH103 균주가 가장 높은 활성을 나타내었다. 계통분석은 Maximum likelihood 방법을 활용하여 결합된 ITS영역, beta-tubulin 및 calmodulin 유전자 염기서열을 분석하여 분리된 균주의 유연관계를 분석하였다. YH103 균주의 배양여과액을 HPLC와 GC/MS SIM을 이용하여 분석한 결과 식물호르몬인 지베렐린 $GA_4$, $GA_7$, $GA_8$ 및 $GA_{19}$가 확인되었다. 최종적으로 YH103 균주의 형태학적 관찰 및 결합된 유전자 염기서열의 분자적 분석을 통해 GA를 생산하는 새로운 Aspergillus tubingensis로 동정되었다.

Coastal plant species Tetragonia tetragonoides (Pall.) Kuntze native to the Dokdo islands was sampled and then 17 endophytic fungi were purely isolated based on morphological differences. The fungal isolates were characterized by their growth properties under NaCl concentration or pH gradient. Culture filtrates of the 17 fungal isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity. As the results, YH103 strain showed the highest plant growth-promoting activity among them. Phylogenetic analysis of the isolates was done by the maximum likelihood method based on partial internal transcribed spacer region (ITS region: contaning ITS1, 5.8S, and ITS2), beta-tubulin (BenA), and calmodulin (CaM) gene sequences. Chromatographic analysis of the strain YH103 culture filtrate showed the existence of gibberellins ($GA_4$, $GA_7$, $GA_8$, and $GA_{19}$). Finally, the strain YH103 was identified as Aspergillus tubingensis by microscopic observation and molecular analysis and, to our knowledge, this is the first report of GAs producing A. tubingensis.

키워드

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피인용 문헌

  1. A strategy for securing unique microbial resources – focusing on Dokdo islands-derived microbial resources pp.2224-4662, 2018, https://doi.org/10.1163/22244662-20181024