Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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A unique thioredoxin reductase plays defensive roles against oxidative, nitrosative and nutritional stresses in Schizosaccharomyces pombe

Schizosaccharomyces pombe의 유일한 치오레독신 환원효소의 산화적, 일산화질소 및 영양 스트레스에 대한 방어적 역할

  • Ji, Dam-Jung (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Lim, Chang-Jin (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Kim, Kyunghoon (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
  • 지담정 (강원대학교 자연과학대학 생화학과) ;
  • 임창진 (강원대학교 자연과학대학 생화학과) ;
  • 김경훈 (강원대학교 자연과학대학 생명과학과)
  • Received : 2016.02.03
  • Accepted : 2016.03.16
  • Published : 2016.03.31
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Abstract

A unique Schizosaccharomyces pombe $TrxR^+$ gene encoding thioredoxin reductase (TrxR) was found to be positively regulated by stress-inducing agents through the stress-responsive transcription factor Pap1. In the present study, the protective roles of S. pombe TrxR were evaluated using the TrxR-overexpressing recombinant plasmid pHSM10. In the presence of hydrogen peroxide ($H_2O_2$) and superoxide anion-generating menadione (MD), S. pombe TrxR increased cellular growth and the total glutathione (GSH) level, while it reduced levels of intracellular reactive oxygen species (ROS). The nitric oxide (NO) levels of the TrxR-overexpressing cells, in the presence of $H_2O_2$ and MD, were maintained to be similar to those of the corresponding non-treated cells. Although S. pombe TrxR was able to scavenge NO generated by sodium nitroprusside (SNP), it had no significant modulating effects on cellular growth, ROS levels, or the total GSH level of SNP-exposed yeast cells, compared with the differences in those of the two non-treated cell cultures. TrxR increased the cellular growth and total GSH level, which were diminished by nitrogen starvation. It also scavenged ROS and NO produced during nitrogen starvation. Taken together, the S. pombe TrxR protects against oxidative, nitrosative, and nutritional stresses.

치오레독신 환원효소(TrxR)를 encoding하는 Schizosaccharomyces pombe의 유일한 $TrxR^+$ 유전자는 스트레스 반응 전사인자인 Pap1의 매개에 의하여 스트레스 유발 인자들에 의하여 양성적으로 조절됨이 발견되었다. 본 연구에서는, TrxR 과잉 발현 재조합 플라스미드 pHSM10을 사용하여 S. pombe TrxR의 방어적 역할들이 평가되었다. 과산화수소($H_2O_2$)와 superoxide anion을 생성하는 menadione (MD)의 존재 하에서, S. pombe TrxR은 세포성장과 총 글루타치온 (GSH) 수준을 증강시키나, 세포 내 활성산소종(ROS) 수준은 감소시켰다. $H_2O_2$와 MD에 의하여 크게 영향 받지 않는 일산화질소(NO) 수준에는 유의성 없는 효과를 보였다. S. pombe TrxR은 sodium nitroprusside(SNP)에 의하여 생성되는 NO를 소거할 수 있었으나, SNP에 노출된 세포들의 성장, ROS 수준이나 총 글루타치온 수준에는 영향을 보이지 않았다. S. pombe TrxR은 질소 결핍(nitrogen starvation)에 의하여 감소되는 세포 성장 및 총 글루타치온 수준을 증가시키며, 질소 결핍에 의하여 생성되는 ROS와 NO를 소거하였다. 요약하건대, S. pombe TrxR은 산화적, 일산화질소 및 영양 스트레스로부터 효모 세포를 보호하지만, 공통적인 기전에 의하지는 않는다.

Keywords

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