Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Melanin Production Inhibitory Activity of the Dendropanax morbifera Leaf Extract Fermented by Lactobacillus plantarum

Lactobacillus plantarum으로 발효시킨 황칠나무 잎 추출물의 피부 미백 관련 효과

  • Im, Do youn (Division of Liberal Arts and Teacher Training, Kwangju Women's University) ;
  • Lee, Kyoung in (Biotechnology Industrialization Center, Dongshin University)
  • 임도연 (광주여자대학교 교양교직과정부) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Received : 2015.10.20
  • Accepted : 2015.11.10
  • Published : 2016.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the tyrosinase inhibitory and melanin production inhibitory activity of the distilled water extract of Dendropanax morbifera leaf (DMW) and the fermented extract by Lactobacillus plantarum (DMF). DMF was prepared by inoculation of L. plantarum after the extraction procedure with distilled water. Fermentation for 48 hours at $37^{\circ}C$ is the most effective condition in this study. In DPPH radical scavenging ability, $SC_{50}$ values of the fermented DMF was $37.9{\mu}g/ml$ as a result of more effective than DMW extract ($52.6{\mu}g/ml$). Moreover, tyrosinase inhibitory activity of DMF showed higher activity than DMW. In nontoxic concentration range, DMF showed strong melanin production inhibitory effect in ${\alpha}$-melanocyte stimulating hormone-stimulated B16F10 cell. As a result, the fermentation of the distilled water extract of D. morbifera leaf by L. plantarum could be applicable to functional materials production for skin-whitening agents.

Keywords

References

  1. Hubert, J., Berger, M., Nepveu, F., Paul, F. and Dayd, J. (2008) Effects of fermentation on the phytochemical composition and antioxidant properties of soy germ. Food Chem. 109: 709-721. https://doi.org/10.1016/j.foodchem.2007.12.081
  2. Kim, N. Y., Lee, Y. D., Cho, S. C., Shin, Y. C. and Lee, H. Y. (2015) Enhancement of anti-inflammation effect by fermentation process in Aronia melanocarpa (Michx.) Elliott extract. Korean J. Medicinal Crop Sci. 22: 475-482.
  3. Kim, B. J., Jo, S. K., Jung, Y. S. and Jung, H. K. (2015) Antidiabetic effects of Allium tuberosum rottler extracts and lactic acid bacteria fermented extracts in type 2 diabetic mice model. Korean J. Food Preserv. 22: 134-144. https://doi.org/10.11002/kjfp.2015.22.1.134
  4. Lee, S. J., Shambhunath, B., Lee, S. J., Jeong, J. E., Koo, B. S., Kim, D. I. and Kim, H. J. (2013) Effects of fermented lotus extracts on the differentiation in 3T3-L1 preadipocytes. J. Korean Med. Obes. Res. 13: 74-83.
  5. Jeong, S. J., Kim, K. H., Son, H. Y. and Yook, H. S. (2014) Effects of extract from fermented flower-buds of Panax ginseng C. A. Meyer on mouse cytokine IL-6, TNF-${\alpha}$ production. Korean J. Food & Nutr. 27: 43-49. https://doi.org/10.9799/ksfan.2014.27.1.043
  6. Choi, Y. W., Lee, C. G., Song, C. H., Seo, Y. C., Kim, J. S., Kim, B. H., Shin, D. H., Yoon, C. S., Lim, H. W. and Lee, H. Y. (2012) Enhancement of low molecular ginsenoside contents in low quality fresh ginseng by fermentation process. Korean J. Medicinal Crop Sci. 20: 117-123. https://doi.org/10.7783/KJMCS.2012.20.2.117
  7. Choi, Y. W., Lim, H. W., Choi, G. P. and Lee, H. Y. (2014) Enhancement of ginsenosides conversion yield by steaming and fermentation process in low quality fresh ginseng. Korean J. Medicinal Crop Sci. 22: 223-230. https://doi.org/10.7783/KJMCS.2014.22.3.223
  8. Kim, N. Y., Park, D. S. and Lee, H. Y. (2015) Effect of antiskin wrinkle and antioxidant of Agastache rugosa Kentz through fermentation process of the lactic acid. Korean J. Medicinal Crop Sci. 23: 37-42. https://doi.org/10.7783/KJMCS.2015.23.1.37
  9. Lee, J. H., Kim, J. I., Choi, H. J. and Lee, J. H. (2014) Antiwrinkle effect of Schizandra chinensis Baillon fermented with Lactobacillus plantarum. J. Soc. Cosmet. Sci. Korea. 40: 365-371.
  10. Marmol, V. del. and Beermann, F. (1996) Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381: 165-168. https://doi.org/10.1016/0014-5793(96)00109-3
  11. Im, D. Y. and Lee, K. I. (2011) Tyrosinase inhibitory activity and melanin production inhibitory activity of the extract and fractions from Paeoniae Radix. Kor. J. Pharmacogn. 42: 323-328
  12. Kim, J. S., Seo, Y. C., Choi, W. Y., Kim, H. S., Kim, B. H., Shin, D. H., Yoon, C. S., Lim, H. W., Ahn, J. H. and Lee, H. Y. (2011) Enhancement of antioxidant activities and whitening effect of Acer mono Sap through nano encapsulation processes. Korean J. Medicinal Crop Sci. 19: 191-197. https://doi.org/10.7783/KJMCS.2011.19.3.191
  13. Tan, X. and Ryu, H. K. (2015) Effects of Dendropanax morbifera leaf extracts on lipid profiles in mice fed a high-fat and high-cholesterol diet. J. Korean Soc. Food Sci. Nutr. 44: 641-648. https://doi.org/10.3746/jkfn.2015.44.5.641
  14. An, N. Y., Kim, J. E., Hwang, D. Y. and Ryu, H. K. (2014) Anti-diabetic effects of aqueous and ethanol extract of Dendropanax morbifera Leveille in streptozotocin-induced diabetes model. J. Nutr. Health. 47: 394-402. https://doi.org/10.4163/jnh.2014.47.6.394
  15. Lee, S. H., Lee, H. S., Park, Y. S., Hwang, B., Kim, J. H. and Lee, H. Y. (2002) Screening of immune activation activities in the leaves of Dendropanax morbifera Lev. Korean J. Medicinal Crop Sci. 10: 109-115.
  16. Choi, J. H., Kim, D. W., Park, S. E., Lee, H. J., Kim, K. M., Kim, K. J., Kim, M. K., Kim, S. J. and Kim, S. (2015) Antithrombotic effect of rutin isolated from Dendropanax morbifera Leveille. J. Biosci. Bioeng. 120: 181-186. https://doi.org/10.1016/j.jbiosc.2014.12.012
  17. Kim, E. S., Lee, J. S. Akram, M., Kim, K. A., Shin, Y. J., Yu, J. H. and Bae, O. N. (2015) Protective activity of Dendropanax morbifera against cisplatin-induced acute kidney injury. Kidney Blood Press Res. 40: 1-12. https://doi.org/10.1159/000368466
  18. Park, S. A., Lee, H. M., Ha, J. H., Jeon, S. H. and Park, S. N. (2014) Inhibitory effects of Dendropanax Morbifera leaf extracts on melanogenesis through down-regulation of tyrosinase and TRP-2. Appl. Chem. Eng. 25: 468-473. https://doi.org/10.14478/ace.2014.1058
  19. Lee, S. Y., Choi, E. J., Bae, D. H., Lee, D. W. and Kim, S. O. (2015) Effects of 1-tetradecanol and ${\beta}$-sitosterol isolated from Dendropanax morbifera Lev. on skin whitening, moisturizing and preventing hair loss. J. Soc. Cosmet. Sci. Korea 41: 73-83.
  20. Otto, F. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biological Chemistry 12: 239-243.
  21. Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  22. Jung, S. W., Lee, N. K., Kim, S. J. and Han, D. S. (1995) Screening of tyrosinase inhibitor from plants. Kor. J. Food Sci. Technol. 27: 891-896.
  23. Shin, K. M., Park, Y. M., Kim, I. T., Hong, S. P., Hong, J. P. and Lee, K. T. (2003) In vitro antiinflammatory activity of amygdalin in murine macrophage Raw 264.7 cells. Kor. J. Pharmacogn. 34: 223-227.
  24. Hosoi, J., Abe, E., Suda, T. and Kuroki, T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478.
  25. Liu, R. H. (2004) Potential synergy of phytochemicals in cancer prevention : mechanism of action. J. Nutr. 3479S-3485S.
  26. Manach, C., Williamson, G., Morand, C., Scalbert, A. and Remesy, C. (2005) Bioavailability and bioefficacy of polyphenols in humans. Am. J. Clin. Nutr. 81: 230S-242S. https://doi.org/10.1093/ajcn/81.1.230S
  27. Yang, E. J., Kim, S. I., Park, S. Y., Bang, H. Y., Jeong, J. H., So, J. H., Rhee, I. K. and Song, K. S. (2012) Fermentation enhances the in vitro antioxidative effect of onion (Allium cepa) via an increase in quercetin content. Food Chem. Toxicol. 50: 2042-2048. https://doi.org/10.1016/j.fct.2012.03.065