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Unrecorded Fungal Species Isolated from Greenhouses Used for Shiitake Cultivation in Korea

표고버섯 재배사에서 분리한 국내 미기록 진균 보고

  • Ahn, Geum Ran (Department of Microbiology and Institute of Biodiverstiy, Dankook University) ;
  • Kwon, Hyuk Woo (Department of Microbiology and Institute of Biodiverstiy, Dankook University) ;
  • Ko, Han Kyu (Forest Mushroom Research Center, National Forest Cooperative Federation) ;
  • Kim, Seong Hwan (Department of Microbiology and Institute of Biodiverstiy, Dankook University)
  • 안금란 (단국대학교 미생물학과 및 생물다양성연구소) ;
  • 권혁우 (단국대학교 미생물학과 및 생물다양성연구소) ;
  • 고한규 (산림조합중앙회 산림버섯연구센터) ;
  • 김성환 (단국대학교 미생물학과 및 생물다양성연구소)
  • Received : 2016.03.17
  • Accepted : 2016.03.18
  • Published : 2016.03.31

Abstract

Fungal contamination is a detrimental factor affecting sawdust media-based shiitake cultivation in greenhouses. During fungal monitoring of greenhouses used for shiitake cultivation, eight fungal species were isolated and identified from indoor air and mushroom flies collected in the greenhouses. The current study reported five species as new in Korea, viz. Ascochyta hordei, Discosia artocreas, Mucor nidicola, Perenniporia medulla-panis, and Pseudozyma prolifica, and confirmed two species, Penicillium charlesii and Penicillium brevicompactum, which were previously recorded in Korea without molecular taxonomic validation. The morphological characteristics and phylogenetic relationships based on nucleotide sequences of the internal transcribed spacer rDNA region or calmodulin gene were described for all identified species.

진균 오염은 톱밥배지를 이용한 실내 표고 재배에 있어서 피해를 주는 요인 중 하나이다. 표고 재배사를 대상으로 진균을 모니터링하는 중, 재배사의 실내공기와 재배사내에서 채집한 버섯파리로부터 여러 진균을 분리 동정하였다. 동정된 진균 중에는 Mucor nidicola, Perenniporia medulla-panis, Discosia artocreas, Pseudozyma prolifica, Ascochyta hordei 등을 국내 미기록종으로 보고하고, Penicillium charlesii와 Penicillium brevicompactum의 국내존재를 확인하였다. 본 논문에서는 이들 미기록 균류에 대한 형태적 특성과 더불어 internal transcribed spacer (ITS) rDNA region 또는 calmodulin 유전자 염기서열에 기반한 계통학적 관계에 대해 기술하였다.

Keywords

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