Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Dihydrobenzofuran Neolignans Isolated from Euonymus alatus Leaves and Twigs Attenuated Inflammatory Responses in the Activated RAW264.7 Macrophage Cells

  • Kim, Na-Hyun (Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology) ;
  • Yang, Min Hye (College of Pharmacy, Pusan National University) ;
  • Heo, Jeong-Doo (Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology) ;
  • Sung, Sang Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Jeong, Eun Ju (Department of Agronomy & Medicinal Plant Resources, College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology)
  • Received : 2015.08.10
  • Accepted : 2015.09.16
  • Published : 2016.03.31
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Abstract

Anti-inflammatory effects of dihydrobenzofuran neolignans isolated from Euonymus alatus leaves and twigs were evaluated in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Six neolignans, (+)-simulanol (1), (+)-dehydrodiconiferyl alcohol (2), (-)-simulanol (3), (-)-dehydrodiconiferyl alcohol (4), (+)-dihydrodehyrodiconiferyl alcohol (5), threo-buddlenol B (6) effectively inhibited the production of nitric oxide (NO) induced by LPS, and the activity of iNOS. (-)-dehydrodiconiferyl alcohol (4), which showed the most potent inhibitory activity, attenuated the activity of iNOS enzyme and also the expression of iNOS and COX-2 proteins. The subsequent production of pro-inflammatory cytokines, interleukin-$1{\beta}$, interleukin-6, tumor necrosis factor-${\alpha}$ and prostaglandin E2 were also inhibited by the pretreatment of RAW264.7 cells with (-)-dehydrodiconiferyl alcohol (4). These neolignans are thought to contribute to anti-inflammatory effects of E. alatus, and expected to be potential candidates to prevent/treat inflammation-related diseases.

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References

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