Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Observation of Scleropthora macrospora Causing Downy Mildew from Zoysiagrass with Leaf Yellowing and Excessive Tillering

총생 증상을 보이는 한국잔디로부터 노균병원균 관찰

  • Han, Muho (Plant & Environment R&D Center, Samsung C&T) ;
  • Kim, Kyung-Duck (Plant & Environment R&D Center, Samsung C&T) ;
  • Pyee, Jaeho (Department of Molecular Biology, Dankook University) ;
  • Choi, Sumin (Southern Forest Resource Center, Korea Forest Research Institute) ;
  • Park, Dae-Sup (Plant & Environment R&D Center, Samsung C&T)
  • 한무호 (삼성물산 식물환경연구소) ;
  • 김경덕 (삼성물산 식물환경연구소) ;
  • 피재호 (단국대학교 분자생물학과) ;
  • 최수민 (국립산림과학원 남부산림자원연구소) ;
  • 박대섭 (삼성물산 식물환경연구소)
  • Received : 2015.11.14
  • Accepted : 2016.02.23
  • Published : 2016.03.31
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Abstract

Zoysiagrass possessing the leaf yellowing with excessive tillers was first found in a golf course in Gyunggi province at last three to four years ago and since then have rapidly spread many golf courses in Korea. The symptom is very similar to that of yellow tuft or downy mildew, which is caused by Sclerophthora macrospora, on cool-season turfgrasses including Kentucky bluegrass and ryegrass. Microscopic study was firstly carried out to look over the presence of the pathogen in the infected leaf and stem tissues. Oospores and sporangia, reported as typical indicators of downy mildew, were obviously observed from the infected leaves. Using a set of primers based on the 18S ribosomal DNA from S. macrospora, the specific DNA fragment corresponding the gene was amplified. This study reveals that S. macrospora might be involved in spread of leaf yellowing of zoysiagrass with excessive tillers.

전국적으로 재배중인 한국잔디에서 확대되고 있는 총생잔디에서 현미경을 이용하여 피시움균의 난포자와 유사하지만 다소 큰 사이즈인 $50{\sim}80{\mu}m$의 난포자를 관찰하였다. 동일 시료들을 암상태의 저온 다습한 조건에서 24시간 증식한 후 현미경으로 관찰한 결과 $80{\sim}100{\mu}m$ 크기의 Sclerophthora macrospora 유주자낭이 확인되었다. 유전자 분석을 통한 확인을 위해 유전자 은행에 등록된 S. macrospora 염기서열 정보를 바탕으로 제작된 프라이머와 감염된 조직에서 추출한 DNA로 PCR 후, S. macrospora 특이적인 밴드가 증폭되는 것을 알 수 있었다. 이러한 결과로 S. macrospora가 한국잔디 총생 증상에 관여하는 가능성이 있을 것으로 사료된다.

Keywords

References

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  2. First Report of Downy Mildew Caused by Sclerophthora sp. on Zoysiagrass (Zoysia japonica) in Korea 2017, https://doi.org/10.1094/PDIS-04-17-0609-PDN