Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Dynamic Patterns of Systemic Innate Immunity and Inflammatory Associated Factors in Experimental Caprine Coccidiosis

  • Tadayon, Shabnam (Department of Pathobiology, School of Veterinary Medicine, Shiraz University) ;
  • Razavi, Seyed Mostafa (Department of Pathobiology, School of Veterinary Medicine, Shiraz University) ;
  • Nazifi, Saeed (Department of Clinical Studies, School of Veterinary Medicine, Shiraz University)
  • Received : 2016.04.06
  • Accepted : 2016.11.09
  • Published : 2016.12.31
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Abstract

The present study was designed to assess the dynamic patterns of pro-inflammatory cytokines, including $IFN-{\gamma}$, $TNF-{\alpha}$, IL-4, IL-6, acute phase protein (${\alpha}1$-acid-glycoprotein, AGP), and an inflammation associated factor (adenosine deaminase; ADA) following experimental caprine coccidiosis. Ten kids aging from 2 to 4 months were infected orally with $5{\times}10^4$ sporulated oocysts and 10 animals served as controls. Blood samples were collected in both groups before infection and at days 3, 7, 14, 21, 28, and 35 post-infection (PI), and the levels of above-mentioned factors were measured. $IFN-{\gamma}$, $TNF-{\alpha}$, IL-4, IL-6, AGP, and ADA activities were significantly higher in infected animals from day 7 PI (P<0.05). In conclusion, the circulatory levels of most systemic inflammatory markers, including pro-inflammatory cytokines ($IFN-{\gamma}$, $TNF-{\alpha}$, IL-4, IL-6), AGP, and ADA increased significantly starting from day 3 to day 7 PI in caprine coccidiosis.

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References

  1. Chartier C, Paraud C. Coccidiosis due to Eimeria in sheep and goats, a review. Small Rumin Res 2012; 103: 84-92. https://doi.org/10.1016/j.smallrumres.2011.10.022
  2. Foreyt WJ. Coccidiosis and cryptosporidiosis in sheep and goats. Vet Clin North Am Food Anim Pract 1990; 6: 655-670. https://doi.org/10.1016/S0749-0720(15)30838-0
  3. Hashemnia M, Khodakaram-Tafti A, Razavi SM, Nazifi S. Experimental caprine coccidiosis caused by Eimeria arloingi: morphopathologic and electron microscopic studies. Vet Res Commun 2012; 36: 47-55. https://doi.org/10.1007/s11259-011-9511-9
  4. Cox FE. Control of coccidiosis: lessons from other sporozoa. Int J Parasitol 1998; 28: 165-179. https://doi.org/10.1016/S0020-7519(97)00166-5
  5. Razavi SM, Hassanvand A. A survey on prevalence of different Eimeria species in goats in Shiraz suburbs. J Vet Res 2006; 61: 373-376.
  6. Tafti AK, Mansourian M. Pathologic lesions of naturally occurring coccidiosis in sheep and goats. Comp Clin Pathol 2008; 17: 87-91. https://doi.org/10.1007/s00580-008-0719-1
  7. Yun CH, Lillehoj HS, Lillehoj EP. Intestinal immune responses to coccidiosis. Dev Comp Immunol 2000; 24: 303-324. https://doi.org/10.1016/S0145-305X(99)00080-4
  8. Iwasaki A, Medzhitov R. Control of adaptive immunity by the innate immune system. Nat Immunol 2015; 16: 343-353. https://doi.org/10.1038/ni.3123
  9. Holdsworth PA, Conway DP, McKenzie ME, Dayton AD, Chapman HD, Mathis GF, Skinner JT, Mundt HC, Williams RB. World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the efficacy of anticoccidial drugs in chickens and turkeys. Vet Parasitol 2004; 121: 189-212. https://doi.org/10.1016/j.vetpar.2004.03.006
  10. Jalila A, Dorny P, Sani R, Salim NB, Vercruysse J. Coccidial infections of goats in Selangor, Peninsular Malaysia. Vet Parasitol 1998; 74: 165-172. https://doi.org/10.1016/S0304-4017(97)00133-7
  11. Frolich S, Entzeroth R, Wallach M. Comparison of protective immune responses to apicomplexan parasites. J Parasitol Res 2011; Article ID 852591: 1-11.
  12. Shivaramaiah C, Barta JR, Hernandez-Velasco X, Tellez G, Hargis B. Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites. Vet Med: 2014; 5: 23-34.
  13. Ovington KS, Alleva LM, Kerr EA. Cytokines and immunological control of Eimeria spp. Int J Parasitol 1995; 25: 1331-1351. https://doi.org/10.1016/0020-7519(95)00069-E
  14. Hashemnia M, Khodakaram-Tafti A, Razavi SM, Nazifi S. Changing patterns of acute phase proteins and inflammatory mediators in experimental caprine coccidiosis. Korean J Parasitol 2011;49: 213-219. https://doi.org/10.3347/kjp.2011.49.3.213
  15. Lillehoj H. Role of T lymphocytes and cytokines in coccidiosis. Int J Parasitol 1998; 28: 1071-1081. https://doi.org/10.1016/S0020-7519(98)00075-7
  16. Hofman FM, Brock M, Taylor CR, Lyons B. IL-4 regulates differentiation and proliferation of human precursor B cells. J Immunol 1988; 141: 1185-1190.
  17. Ibarra-Velarde F, Alcala-Canto Y. Downregulation of the goat ${\beta}$-defensin-2 gene by IL-4 in caprine intestinal epithelial cells infected with Eimeria spp. Parasitol Res 2007; 101: 613-618. https://doi.org/10.1007/s00436-007-0523-x
  18. Baumann H, Gauldie J. The acute phase response. Immunol Today 1994; 15: 74-80. https://doi.org/10.1016/0167-5699(94)90137-6
  19. Tothova C, Nagy O, Seidel H, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Vet Med Czech 2014; 59: 163-180. https://doi.org/10.17221/7478-VETMED
  20. Lee KW, Lillehoj HS, Jang SI, Lee SH. Effects of various field coccidiosis control programs on host innate and adaptive immunity in commercial broiler chickens. Korean J Poult Sci 2012; 39: 17-25. https://doi.org/10.5536/KJPS.2012.39.1.017
  21. Linden J. New insights into the regulation of inflammation by adenosine. J Clin Invest 2006; 116: 1835-1837. https://doi.org/10.1172/JCI29125
  22. Hasko G, Cronstein BN. Adenosine: an endogenous regulator of innate immunity. Trends Immunol 2004; 25: 33-39. https://doi.org/10.1016/j.it.2003.11.003

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