The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Molecular Phylogenetic Study of the Endangered Land Snail Satsuma myomphala Based on Metallothionein Gene.

Metallothionein 유전자를 기초로 한 멸종위기 육상 달팽이 Satsuma myomphala (거제외줄달팽이) 의 분자계통학적 연구

  • Sang, Min Kyu (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Kang, Se Won (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Hwang, Hee-Ju (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Chung, Jong Min (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Song, Dae Kwon (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Min, Hye Rin (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Park, Jie Eun (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Ha, Hee Cheol (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Lee, Hyun Jun (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Hong, Chan Eui (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Ahn, Young Mo (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Park, So Young (Biodiversity Conservation & Change Research Division, Nakdonggang National Institute of Biological Resources) ;
  • Park, Young-Su (Department of Nursing, College of Medicine, Soonchunhyang University) ;
  • Park, Hong Seog (Research Institute, GnC BIO Co., LTD.) ;
  • Han, Yeon Soo (College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Jun Sang (Institute of Environmental Research, Kangwon National University) ;
  • Lee, Yong Seok (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University)
  • 상민규 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 강세원 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 황희주 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 정종민 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 송대권 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 민혜린 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 박지은 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 하희철 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 이현준 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 홍찬의 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 안영모 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 박소영 (국립낙동강생물자원관 다양성보전.변화연구부) ;
  • 박영수 (순천향대학교 의과대학 간호학과) ;
  • 박홍석 ((주)지앤시바이오) ;
  • 한연수 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 이준상 (강원대학교 환경연구소) ;
  • 이용석 (순천향대학교 자연과학대학 생명시스템학과)
  • Received : 2016.12.12
  • Accepted : 2016.12.30
  • Published : 2016.12.31
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Abstract

Metallothionein (MT) family of metal-binding proteins are involved in maintaining homeostasis and heavy metal poisoning. Recently, MT has been considered as a biomarker that can identify a particular species, very similar to the use of cytochrome oxidase I (COI) gene. Satsuma myomphala species of land snails have been reported from North-East Asia, including South Korea and Japan. In particular, the land snail species have been known from only a limited area of Geoje Island, Gyeongsangnam-do province of South Korea. Genetic studies of S. myomphala has been limited with only 6 nucleotide, 2 protein registered on the NCBI server. For elucidating the genetic information of S. myomphala, we conducted RNA sequencing analysis using Illumina HiSeq 2500 next-generation platform. We screened the MT gene from the RNA-Seq database to confirm the molecular phylogenetic relationship. After sequencing, the de novo analysis and clustering generated 103,774 unigenes. After annotation against PANM database using BLAST program, we obtained MT sequence of 74 amino acid residues containing the coding region of 222 bp. Based on this sequence, we found about 53 sequences using the BLAST program in NCBI nr database. Using ClustalX alignment, Maximum-Likehood Tree of MEGA program, we confirmed the molecular phylogenetic relationships that showed similarity with mollusks such as Helix pomatia and H. aspersa, Megathura crenulata.

Keywords

References

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