대한방사선기술학회지:방사선기술과학 (Journal of radiological science and technology)

  • 제39권4호
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  • Pages.623-630
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  • 2016
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  • 2288-3509(pISSN)
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  • 2384-1168(eISSN)
대한방사선과학회 (Korean Society of Radiological Science)
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방사선 피폭 시 간세포의 손상 기전과 알리인의 방어효과

Study on Radioprotection of Alliin and Damage Mechanism in Hepatocyte After Irradiation

  • 지태정 (가야대학교 방사선학과) ;
  • 김원태 (가야대학교 방사선학과)
  • Ji, Tae-Jeong (Department of Radiological Science, Kaya University) ;
  • Kim, Won-Tae (Department of Radiological Science, Kaya University)
  • 투고 : 2016.11.10
  • 심사 : 2016.12.20
  • 발행 : 2016.12.31
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초록

방사선 피폭에 의한 간조직의 장해는 경화성 위축으로 복수가 발생하고 황달과 피로감이 수반된다. 이러한 장해에는 간세포의 형태학적 변화가 동반되고 소기관들의 손상이 연관되어 있다. 본 연구는 이러한 소기관들의 형태적 손상을 알아보고자 하였다. 특히 에너지 대사와 관련된 미토콘드리아와 세포질그물 핵막을 중심으로 관찰하였으며, 방사선 방어제 연구와 병행하였다. 방어제는 혈류 증가 작용이 있는 알리인을 투여하였다. 세포 관찰은 투과전자현미경을 활용하였다. 실험결과, 7 Gy 전신 조사에서 간세포의 핵막이 비후되고, 소기관들 주변에 염증성 변화가 관찰되었다. 20일 경과된 세포에서는 과산화소체와 미토콘드리아 막이 손상되었고 세포질에서 공포가 확인되었다. 30일 경과된 세포에서는 거대한 지방세포들이 관찰되었고. 조면소포체에는 리보솜이 분리된 것을 확인하였다. 알리인 투여 후 간세포에서는 핵막 주변에 많은 수의 조면소포체들이 선명하게 관찰되었고 핵막도 비후되지 않았다. 세포질에는 공포화되거나 염증성 증후들이 관찰되지 않았다. 미토콘드리아의 막손상도 없었으며 글리코겐도 정상적으로 확인되었다. 따라서 일부 방어효과가 있는 것으로 확인되었다.

Liver tissue damage by a radiation exposure caused a jaundice and ascitic fluid e form harden atrophy. The reason for this lies in morphological damage of a liver cells. This study tried that observe damage mechanism of the cell organelles. It was especially observed mitochondria, endoplasmic reticulum and nuclear membrane associated with energy metabolizable. also, This study had with a radio-protector development research at the same time. Radio-protector was used to alliin that has an blood flow increase. Cell observation make used of transmission electron microscope(TEM). The result of an experiment, 7Gy of whole body irradiation was caused an inflammation in cell organelles and hypertrophy of the nucleus membrane. After 20 days, The hepatocyte has been observed in a damaged membrane on peroxisome, mitochondria and vacuole of the cell organelles. After 30 days, The hepatocyte has been observed in disconnected ribosomes on a rough endoplasmic reticulum. There was looked a giant lipoblast. There was clearly normal observed a mitochondria and nucleus membrane in the hepatocyte after alliin injection. aslo, It was no damaged the nucleus membrane. therefore, It was identified portion a radio-protector effect from alliin.

키워드

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