Journal of Acupuncture Research

  • 제33권1호
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  • Pages.37-46
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  • 2016
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
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Formalin으로 유도된 통증 모델에서 태계혈(太谿穴)(KI3)의 원엽 모고채(Drosera rotundifolia L.) 약침의 진통효과

Analgesic Effects of Drosera rotundifolia L. Pharmacopuncture at Taegye(KI3) Acupoint on Formalin-induced Pain

  • 오세정 (대구한의대학교 한의과대학 침구의학과) ;
  • 김재수 (대구한의대학교 한의과대학 침구의학과) ;
  • 이윤규 (대구한의대학교 한의과대학 침구의학과) ;
  • 이현종 (대구한의대학교 한의과대학 침구의학과)
  • Oh, Se Jung (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Hyun Jong (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daegu Haany University)
  • 투고 : 2016.02.12
  • 심사 : 2016.03.08
  • 발행 : 2016.03.18
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초록

Objectives : The objective of this study is to evaluate the analgesic effects of Drosera rotundifolia L. pharmacopuncture on formalin-induced pain in Sprague-Dawley(SD) rats. Methods : In this experiment there were four groups, each with six SD rats. In the normal group (NOR), normal saline $40{\mu}L$ was injected at right KI3, and normal saline $40{\mu}L$ was injected at right hindpaw 35 minutes later. In the control group (CON), normal saline $40{\mu}L$ was injected at right KI3, and formalin 5 % $40{\mu}L$ was injected at right hindpaw 35 minutes later. In the Drosera rotundifolia L. pharmacopuncture 3 % group (DP3), Drosera rotundifolia L. pharmacopuncture 3 % $40{\mu}L$ was injected at right KI3, and formalin 5 % $40{\mu}L$ was injected at right hindpaw 35 minutes later. In the Drosera rotundifolia L. pharmacopuncture 5 % group (DP5), Drosera rotundifolia L. pharmacopuncture 5 % $40{\mu}L$ was injected at right KI3, and formalin 5 % $40{\mu}L$ was injected at right hindpaw 35 minutes later. We analyzed ultrasonic vocalization (USV), Substance P, aspartate aminotransferase(AST), and alanine aminotransferase(ALT). Results : In the early phase of USV, both DP3 and DP5 had an analgesic effect. In the late phase, DP5 had an analgesic effect compared to CON. Substance P in DP5 was significantly decreased compared to CON. In regards to blood AST and ALT, there was no significant difference among NOR, CON, DP3 or DP5. Conclusion : These results suggest that Drosera rotundifolia L. pharmacopuncture helps to reduce formalin-induced pain. It's mechanism is related to substance P, and Drosera rotundifolia L. pharmacopuncture has no influence on liver toxicity.

키워드

참고문헌

  1. Statics Korea. Allowance Status of Disease's Sub Category about Frequence(2014) [Internet]. Daejeon : Statistics Korea. [cited 2015 Octorber 28]. available from : http : //kosis.kr/statHtml/statHtml.do?orgId=350&tblId=DT_35001_A81211&conn_path=I2
  2. Park JE, Jung HJ, Kim AR, Jung SY, Hwang HS, Choi SM. Current State of Pain Treatment in Oriental Medicine. Journal of Korean Medicine. 2011 ; 32(2) : 23-41.
  3. Korean Pharmacopuncture Institute. Guidelines for Korean Institute of Herbal Acupuncture. Seoul : Hansung. 1999 : 13.
  4. Korean Acupuncture & Moxibustion Society Textbook Compilation Committee. The Acupunture and Moxibustion Medicine. Gyeonggi : Jipmoondang. 2012 : 230-1.
  5. Korean Pharmacopuncture Institute. Pharmacopuncturology. 2nd ed. Seoul : Elsevier Korea. 2011 : 6-8.
  6. Yang KR, Song HS. A Comparative study of Warm needling and Bee Venom Pharmacopuncture on Osteoarthritis of the Knee - A Randomized Controlled Trial. Journal of Pharmacopuncture. 2008 ; 11(2) : 21-31. https://doi.org/10.3831/KPI.2008.11.2.021
  7. Lee GJ, Song YK, Lim HH. Effect of Bee Venom Pharmacopuncture and Scolopendra Subspinipes Pharmacopuncture on Functional Recovery and Anti-inflammation after Sciatic Crushed Nerve Injury in Rats. J Oriental Rehab Med. 2013 ; 23(2) : 17-31.
  8. Ku JY. Comparison of the Effects between Sweet Bee Venom and Scolopendrid Pharmacopuncture on Carpal Tunnel Syndrome (Randomized, Controlled Clinical Trial) [dissertation]. Busan : Dongeui Univ. 2011. Korean.
  9. Park MH, Lim ST, Choi SW. The Clinical Study on a Case of Transverse Myelitis with Bee Venom andHominis Placenta Herbal Acupuncture. Journal of Pharmacopuncture. 2005 ; 8(2) : 17-22. https://doi.org/10.3831/KPI.2005.8.2.017
  10. Moon GS. Ingredient and Use of Medicinal Herb. Seoul : Ilweolseogak. 1996 : 321-2.
  11. The Compilation Committee. Chinese Medicinal Herb Pictorial Book. Seoul : Yeogang. 1994 :1154.
  12. Melzig MF, Pertz HH, Krenn L. Anti-inflammatory and Spasmolytic Activity of Extracts from Droserae Herba. Phytomedicine. 2001 ; 8(3) : 225-9. https://doi.org/10.1078/0944-7113-00031
  13. Fukushima K, Nagai K, Hoshi Y et al. Drosera rotundifolia and Drosera tokaiensis Suppress the Activation of HMC-1 Human Mast Cells. J Ethnopharmacol. 2009 ; 125(1) : 90-6. https://doi.org/10.1016/j.jep.2009.06.009
  14. Ko HK. Transpositional Acupoints of the Rat. The Acupuncture. 1999 ; 16(3) : 115-22.
  15. Yim YK, Ahn BC, Park DS et al. Review of the Formalin Test to Study Analgesic Effect and the Mechanism of the Analgesic Effect. The Acupuncture. 1999 ; 16(4) : 1-15.
  16. Ellman GL. Tissue Sulfhydryl Groups. Arch. Biochem. Biophys. 1959 ; 82(1) : 70-7. https://doi.org/10.1016/0003-9861(59)90090-6
  17. Reitman S, Frankel S. A Colorimetric Method for the Determination of Serum Glutamic oxalacetic and Glutamic pyruvic transaminase. Am J Clin Pathol. 1957 ; 28(1) : 56-63. https://doi.org/10.1093/ajcp/28.1.56
  18. Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL. Harrison's Principles of Internal Medicine. 17th ed. Seoul : Jungdam. 2010 : 95-1016.
  19. Merskey H, Albe-Fessard DG, Bonica JJ et al. Pain Terms : A List with Definitions and Notes on Usage. Pain. 1979 ; 6(3) : 249-52.
  20. Kim SO. Studies on the Mechanism of Pain. BioWave. 2006 ; 8(3) : 1-6.
  21. Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The Formalin Test : an Evaluation of the Method. Pain. 1992 ; 51(1) : 5-17. https://doi.org/10.1016/0304-3959(92)90003-T
  22. Jeong IJ. Analgesic Effect of Poly(d,l-lacticco-glycolic acid)-encapsulated Bee Venom Acupuncture at ST36 acupoint on Formalininduced Pain in Rats [dissertation]. Seoul : Kyung Hee Univ. 2008. Korean.
  23. Choi WJ, Kim KB, Kim KH, Kim JH. Analgesic Effect of Clematidis Radix(CR) Herbalacupuncture on the Test Rats with Induced Acute Pain. The Acupuncture. 2009 ; 26(6) : 91-100.
  24. Dictionary of New OrientalMedicine Committee. Dictionary of New Oriental Medicine. Seoul : Yeogang. 2005 : 624, 843, 1167-8.
  25. Portfors CV. Types and Functions of Ultrasonic Vocalizations in Laboratory Rats and Mice. J Am Assoc Lab Anim Sci. 2007 ; 46(1) : 28-34.
  26. Williams WO, Kriskin D, Mott KM. Ultrasonic Sound as an Indicator of Acute Pain in Laboratory Mice. J Am Assoc Lab Anim Sci. 2008 ; 47(1) : 8-10.
  27. Han JS, Bird GC, Li W, Jones J, Neugebauer V. Computerized Anlysis of Audible and Ultrasonic Vocalizations of Rats as a Standardized Measure of Pain-related Behaver. J Neurosci Methods. 2005 ; 141(2) : 261-9. https://doi.org/10.1016/j.jneumeth.2004.07.005
  28. Buck CL. Neurobehavioral Correlates of Ultrasonic Vocalization in the Rat [dissertation]. San Diego : Univ of California. 2014. English.
  29. Jeon JH. Ultrasound Vocalizationsin Laboratory Rats. Bulletin of the animal biotechnology. 2008 ; 1 : 47-50.
  30. Martin JL, Njoku DB. Metabolism and Toxicity of Modern Inhaled Anesthetics. In : Miller's Anesthesia. 6th edition. Philadelphia : Elsevier Churchill Livingstone. 2005 : 231-72.
  31. Njoku D, Laster MJ, Gong DH, Eger EI 2nd, Reed GF, Martin JL. Biotransformation of Halothane, Enflurane, Isoflurane, and Desflurane to Trifluoroacetylated Liver Proteins : Association Between Protein Acylation and Hepatic Injury. Anesth Analg. 1997 ; 84(1) : 173-8. https://doi.org/10.1213/00000539-199701000-00031
  32. Park JT, Lee YB, Kim JS, Ryu H, Lim HK. Acute Liver Injury after Sevoflurane Anesthesia -A Case Report. Korean J Anesthesiol. 2009 ; 57(2) : 221-4. https://doi.org/10.4097/kjae.2009.57.2.221
  33. Choi KH. Dictionary of Checkup Medicine. 2nd ed. Seoul : Daehan Medical Literature. 2011 : 14, 127.

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