Biomaterials and Biomechanics in Bioengineering

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Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion

  • Chien, Hsiu-Wen (Department of Chemical Engineering, National Taiwan University) ;
  • Keng, Ming-Chun (Department of Chemical Engineering, National Taiwan University) ;
  • Chen, Hsien-Yeh (Department of Chemical Engineering, National Taiwan University) ;
  • Huang, Sheng-Tung (Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Science and Technology) ;
  • Tsai, Wei-Bor (Department of Chemical Engineering, National Taiwan University)
  • Received : 2015.10.01
  • Accepted : 2016.02.15
  • Published : 2016.03.25
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Abstract

A surface resisting protein adsorption and cell adhesion is highly desirable for many biomedical applications such as diagnostic devices, biosensors and blood-contacting devices. In this study, a surface conjugated with sulfobetaine molecules was fabricated via the click reaction for the anti-fouling purpose. An alkyne-containing substrate (Alkyne-PPX) was generated by chemical vapor deposition of 4-ethynyl-[2,2]paracyclophane. Azide-ended mono-sulfobetaine molecules were synthesized and then conjugated on Alkyne-PPX via the click reaction. The protein adsorption from 10% serum was reduced by 57%, while the attachment of L929 cells was reduced by 83% onto the sulfobetaine-PPX surface compared to the protein adsorption and cell adhesion on Alkyne-PPX. In conclusion, we demonstrate that conjugation of mono-sulfobetaine molecules via the click chemistry is an effective way for reduction of non-specific protein adsorption and cell attachment.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology of Taiwan

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