Journal of Nutrition and Health

  • 제49권1호
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  • Pages.59-62
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  • 2016
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
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혈관 플라그 형성 저해단백질에 대한 아연의 기능

Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation

  • 신미영 (안동대학교 생활과학대학 식품영양학과) ;
  • 권인숙 (안동대학교 생활과학대학 식품영양학과)
  • Shin, Mee-Young (Department of Food science and Nutrition, Andong National University) ;
  • Kwun, In-Sook (Department of Food science and Nutrition, Andong National University)
  • 투고 : 2016.02.01
  • 심사 : 2016.02.17
  • 발행 : 2016.02.29
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초록

Purpose: Zinc, a biomineral present within and outside cells, manages various cellular mechanisms. In this study, we examined whether zinc was involved in vascular smooth muscle cell (VSMC) calcification via regulation of calcification inhibitor protein, osteopontin (OPN). Methods: Rat aorta cell line (A7r5 cells) and primary vascular smooth muscle cells (pVSMCs) from rat aorta were cultured with phosphate (1-5 mM) and zinc ($0-15{\mu}M$) as appropriate, along with osteoblasts (MC3T3-E1) as control. The cells were then stained for Ca and P deposition for calcification examination as well as osteopontin expression as calcification inhibitor protein was measured. Results: Both Ca and phosphate deposition increased as the addition of phosphate increased. In the same manner, the expression of osteopontin was upregulated as the addition of phosphate increased in both cell types. When zinc was added, Ca and P deposition decreased in VSMCs, while it increased in osteoblasts. Conclusion: The results imply that zinc may prevent VSMC calcification by stimulating calcification inhibitor protein OPN synthesis in VSMCs.

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참고문헌

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