Mycobiology

  • 제44권4호
  • /
  • Pages.338-342
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  • 2016
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  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses

  • Kwak, A-Min (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, Sang-Yeop (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • 투고 : 2016.11.17
  • 심사 : 2016.11.28
  • 발행 : 2016.12.30
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초록

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.

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참고문헌

  1. Maeda YY, Takahama S, Yonekawa H. Four dominant loci for the vascular responses by the antitumor polysaccharide, lentinan. Immunogenetics 1998;47:159-65.
  2. Ngai PH, Ng TB. Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells. Life Sci 2003;73:3363-74. https://doi.org/10.1016/j.lfs.2003.06.023
  3. Yang BK, Kim DH, Jeong SC, Das S, Choi YS, Shin JS, Lee SC, Song CH. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci Biotechnol Biochem 2002;66:937-42. https://doi.org/10.1271/bbb.66.937
  4. Morita K, Kobayashi S. Isolation, structure, and synthesis of lenthionine and its analogs. Chem Pharm Bull (Tokyo) 1967;15:988-93. https://doi.org/10.1248/cpb.15.988
  5. Yasumoto K, Iwami K, Mitsuda H. A new sulfur-containing peptide from Lentinus edodes acting as a precursor for lenthionine. Agric Biol Chem 1971;35:2059-69. https://doi.org/10.1080/00021369.1971.10860187
  6. Hatvani N. Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. Int J Antimicrob Agents 2001;17:71-4. https://doi.org/10.1016/S0924-8579(00)00311-3
  7. Subramaniyam R, Vimala R. Solid state and submerged fermentation for the production of bioactive substances: a comparative study. Int J Sci Nat 2012;3:480-6.
  8. Barros L, Pereira C, Ferreira IC. Optimized analysis of organic acids in edible mushrooms from Portugal by ultra fast liquid chromatography and photodiode array detection. Food Anal Methods 2013;6:309-16. https://doi.org/10.1007/s12161-012-9443-1
  9. Kwak AM, Min KJ, Lee SY, Kang HW. Water extract from spent mushroom substrate of Hericium erinaceus suppresses bacterial wilt disease of tomato. Mycobiology 2015;43:311-8. https://doi.org/10.5941/MYCO.2015.43.3.311
  10. Al-Wahsh IA, Horner HT, Palmer RG, Reddy MB, Massey LK. Oxalate and phytate of soy foods. J Agric Food Chem 2005; 53:5670-4. https://doi.org/10.1021/jf0506378
  11. Gao Z, Shao J, Sun H, Zhong W, Zhuang W, Zhang Z. Evaluation of different kinds of organic acids and their antibacterial activity in Japanese Apricot fruits. Afr J Agric Res 2012;7:4911-8.
  12. Aletor VA. Compositional studies on edible tropical species of mushrooms. Food Chem 1995;54:265-8. https://doi.org/10.1016/0308-8146(95)00044-J
  13. Bonsmann SS, Walczyk T, Renggli S, Hurrell RF. Oxalic acid does not influence nonhaem iron absorption in humans: a comparison of kale and spinach meals. Eur J Clin Nutr 2008;62:336-41. https://doi.org/10.1038/sj.ejcn.1602721
  14. Bender S, Dumitrache-Anghel CN, Backhaus J, Christie G, Cross RF, Lonergan GT, Baker WL. A case for caution in assessing the antibiotic activity of extracts of culinary-medicinal Shiitake mushroom [Lentinus edodes (Berk.) Singer] (Agaricomycetideae). Int J Med Mushrooms 2003;5:31-5. https://doi.org/10.1615/InterJMedicMush.v5.i1.40
  15. Cessna SG, Sears VE, Dickman MB, Low PS. Oxalic acid, a pathogenicity factor for Sclerotinia sclerotiorum, suppresses the oxidative burst of the host plant. Plant Cell 2000;12:2191-200. https://doi.org/10.1105/tpc.12.11.2191