International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Reactive Oxygen Species are Involved in Y-27632-induced Neurite Outgrowth in PC12 Cells

  • Park, So Yeong (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Moon, Seong Ah (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • An, Jeong Mi (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Kim, Du sik (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Seo, Jeong Taeg (Department of Oral Biology, Yonsei University College of Dentistry)
  • Received : 2016.11.21
  • Accepted : 2016.12.13
  • Published : 2016.12.31
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Abstract

Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.

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References

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