Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Unrecorded fungi isolated from indoor air of cultivation houses used for field test of a newly bred domestic shiitake cultivar

표고 현장적응 시험 버섯 재배사내 공기에서 검출한 국내 미기록 진균 보고

  • 안금란 (단국대학교 미생물학과) ;
  • 안홍석 (단국대학교 미생물학과) ;
  • 권혁우 (단국대학교 생물다양성연구소) ;
  • 고한규 (산림조합중앙회 산림버섯연구센터) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2016.10.24
  • Accepted : 2016.11.10
  • Published : 2016.12.31
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Abstract

Four fungal species, during indoor air monitoring for fungi that possibly affect the field testing of a newly bred shiitake cultivar in cultivation houses located in Cheongyang, Chungnam Province and Jangheung, Jeonnam Province. Of these species, two are known to be plant pathogens and the other two are saprobes but potent contaminators in the mushroom cultivation environment. This study reports the morphological characteristics and phylogenetic relationships of these four species based on nucleotide sequences of the internal transcribed spacer (ITS) and 18S rDNA region, including their known information.

청양과 장흥에 소재한 버섯 재배사에서 새로 육종된 표고 품종의 현장 실증검증 도중 문제를 일으킬 수 있는 잠재적 진균을 파악하고자 재배사내 공기 모니터링을 수행하여 오던 중 국내에 기록이 없는 Mortierella parvispora, Doratomyces purpureofuscus, Periconia byssoides, Periconia pseudobyssoides 등 네 종의 진균을 분리하여 동정하였다. 이중 두 종은 식물병원균으로 알려진 종이었고 다른 두 종은 부생성 균으로 다량의 포자를 생산하고 버섯재배 환경에서 오염균으로 작용할 가능성이 있는 균이었다. 본 연구에서는 이들 동정된 진균에 대한 형태적 특성, internal transcribed spacer (ITS) 와 18S rDNA region 염기서열 분석에 기반한 계통학적 관계, 그리고 알려진 정보 등에 대하여 보고하고자 한다.

Keywords

References

  1. Ahn GR, Kwon HW, Ko HK, Kim SH. 2015. Unrecorded fungal species isolated from greenhouses used for shiitake cultivation in Korea. Kor J Mycol. 44(1):8-15. (in Korean) https://doi.org/10.4489/KJM.2016.44.1.8
  2. Andersen AA. 1958. New sampler for the collection, sizing and enumeration of viable airborne particles. J Bacteriol. 76:471-484.
  3. Beyer DM, 2002. Weed and Indicator Moulds. Chapter in Mushroom integrated pest management handbook. Pennsylvania State University.
  4. Bunning SE, Griffiths DA. 1984. Spore development in species of Periconia II. P. byssoides and P. igniaria. Trans Br Mycol Soc. 82: 397-404. https://doi.org/10.1016/S0007-1536(84)80002-9
  5. Chouaki T, Lavarde V, Lachaud L, Raccur CP, Hennequi C. 2002. Invasive infections due to Trichoderma species: report of 2 cases, findings of in vitro susceptibility testing, and review of the literature. Clin Infect Dis. 35:1360-1367. https://doi.org/10.1086/344270
  6. Chigoziri E, Ekefan EJ. 2013. Seed borne fungi of Chilli Pepper (Capsicum frutescens) from pepper producing areas of Benue State, Nigeria. Agric Biol J N Am. 4:370-374. https://doi.org/10.5251/abjna.2013.4.4.370.374
  7. Eroshin VK, Dedyukhina EG. 2002. Effect of lipids from Mortierella hygrophila on plant resistance to phytopathogens. World J Microbiol Biotechnol. 18:165-167. https://doi.org/10.1023/A:1014429527591
  8. Fagan SM, Fergus C. 1984. Extracellular enzymes of some additional fungi associated with mushroom culture. Mycopathologia 87:67-70. https://doi.org/10.1007/BF00436631
  9. Fletcher JT, White PF, Gaze RH, 1994. Mushrooms: Pest & Disease Control, 2nd edition. Intercept: Andover.
  10. Gradisar H, Kern S, Friedrich J. 2000. Keratinase of Doratomyces microspores. Appl Microbiol Biotechnol. 53:196-200. https://doi.org/10.1007/s002530050008
  11. Higashiyama K, Fujikawa S, Park YE, Shimizu S. 2002. Production of arachidonic acid by Mortierella fungi. Biotechnol Bioprocess Eng. 7:252-262. https://doi.org/10.1007/BF02932833
  12. Jung EB, Jo JH, Cho SM. 2008. Nutritional component and anticancer of various extracts from Haesongi mushroom (Hypsizigus marmoreus). J Korean Soc Food Sci Nutr. 37:1395-1400. https://doi.org/10.3746/jkfn.2008.37.11.1395
  13. Kang MY, Kim S, Yun HJ, Nam SH. 2004. Antioxidative activity of the extracts from browned oak mushroom (Lentinus edodes) with unmarketable quality. Korean J Food Sci Technol. 36: 648-654.
  14. Kim CS, Park MS, Kim SC, Maekawa N, Yu SH. 2012. Identification of Trichoderma, a competitor of shiitake mushroom (Lentinula edodes), and competition between Lentinula edodes and Trichoderma species in Korea. Plant Pathol J. 28:137-148. https://doi.org/10.5423/PPJ.2012.28.2.137
  15. Kim SH, Uzunovic A, Breuil C. 1999. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl Eviron Microbiol. 65:287-290.
  16. Kolomiets T, Pankratova L, Mukhina Z, Kassanelly D, Matveeva T, Bogomaz D, Berner D. 2008. First report of leaf spot caused by Periconia igniaria on yellow sarthistle in Russia. Plant Dis. 92:983.
  17. Kwon HW, Yun YH, Kim JY, Kim SH, Ko HK. 2015. New records of fungi isolated from indoor air of greenhouse used for shiitake cultivation in Korea. Kor J Mycol. 43(1):58-63. (in Korean) https://doi.org/10.4489/KJM.2015.43.1.58
  18. Latge JP. 1999. Aspergillus fumigatus and Aspergillosis. Clin Microbiol Rev. 12(2):310-350.
  19. Lee MR, Oh DS, Wee AJ, Yun BS, Jang SA, Sung CK. 2014. Antiobesity effects of Lentinus edodes on obese mice induced by high fat diet. J Korean Soc Food Sci Nutr. 43:194-199. https://doi.org/10.3746/jkfn.2014.43.2.194
  20. Liao YM. 1993. Microorganisms contaminated in the process of cultivation and their effect on the production of shiitake. J Agric Res China. 42:187-199.
  21. Markovskaja S, Kacergius A. 2014. Morphological and molecular characterisation of Periconia pseudobyssoides sp. nov. and closely related P. byssoides. Mycol Progress 13:291. https://doi.org/10.1007/s11557-013-0914-6
  22. Morton FJ, Smith G. 1963. The genera Scopulariopsis Bainier, Microascus Zukal and Doratomyces Corda. Mycological Papers 86:1-96.
  23. Nanba H, Mori K, Toyomasu T, Kuroda H. 1987. Antitumor action of shiitake (Lentinus edodes) fruit bodies orally administered to mice. Chem Pharm Bull (Tokyo). 35:2453-2458. https://doi.org/10.1248/cpb.35.2453
  24. Pengra RM, Cole MA, Alexander M. 1969. Cell walls and lysis of Mortierella parvispora hyphae. J Bacteriol. 97:1056-1061.
  25. Peregrine WTH, Ahmad KB. 1982. Brunei: A first annotated list of plant diseases and associated organisms. Phytopathological Papers 27:1-87.
  26. Pitt JI. 1994. The current role of Apsergillus and Penicillium in human and animal health. J Med Vet Mycol. 32:17-32. https://doi.org/10.1080/02681219480000701
  27. Ribes JA, Vanover-Sams CL, Baker DJ. 2000. Zygomycetes in human disease. Clin Microbiol Rev. 13:236-301. https://doi.org/10.1128/CMR.13.2.236-301.2000
  28. Seo SY, Ahn M., Choi SR, Song EJ, Choi MK, Kim YS. 2011. Analysis of nutritional compositions and biological activity of Agrocybe aegerita. J Mushroom Sci Prod. 9:116-122.
  29. Togashi I, Itoh K, Gisusi S, Harada A. 1997. Distribution of airborne fungi in fruiting houses for the sawdust-based cultivation of Lentinus edodes. J Hokkaido For Prod Res Inst. 11:1-4.
  30. Um S, Jin G, Park KW, Yu Y, Park KM. 2010. Physiological activity and nutritional composition of Pleurotus species. Korean J Food Sci Technol. 42:90-96.
  31. Wu Q, Li Y, Li Y, Gao S, Wang M, Zhang T, Chen J. 2013. Identification of a novel fungus, Leptosphaerulina chartarum SJTU59 and characterization of its xylanolytic enzymes. PLoS One. 8:e73729. https://doi.org/10.1371/journal.pone.0073729

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