한국가시화정보학회지 (Journal of the Korean Society of Visualization)

  • 제14권3호
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  • Pages.38-45
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  • 2016
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  • 1598-8430(pISSN)
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  • 2093-808X(eISSN)
한국가시화정보학회 (The Korean Society of Visualization)
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Alignment of Microbeads Using Spinning Helical Minichannel Cartridge

  • Kim, Subin (Department of Mechanical Engineering, Graduate School, Kookmin University) ;
  • Prasad, Bibin (Department of Mechanical Engineering, Graduate School, Kookmin University) ;
  • Kim, Jung Kyung (School of Mechanical Systems Engineering, Kookmin University)
  • 투고 : 2016.12.13
  • 심사 : 2016.12.26
  • 발행 : 2016.12.31
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초록

Separation of particles based on different sizes, detection of pathogenic bacteria and isolation of leukocytes from whole blood are typical applications of spiral or helical microchannels. The present study focuses on developing a CD4+ T-cell counting device for monitoring HIV/AIDS patients with the aid of a helical minichannel used for a sample cartridge. For the experiment, $10{\mu}m$ sized microbeads were used for visualization with a fluorescence imaging system. Alignment of microbeads was investigated in a stationary and spinning sample cartridge filled with glycerol-water mixtures of different densities. The helical minichannel was spun using a DC motor controlled by an Arduino board with a Bluetooth shield. It was found that when the sample cartridge was made stationary, no bead alignment was achieved for a medium with density (0% and 20% glycerol) lower than that of the beads, but when it was spun at 2000-3000 rpm for 1-4 min, an alignment was obtained at the top of the channel facilitating optical detection and enumeration of those microbeads. Since an alignment of microbeads was achieved for a medium with density as that of blood plasma, the same approach can be applied for aligning and counting CD4+ T-lymphocytes in whole blood samples collected from patients.

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참고문헌

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