어미 크기 및 복부팽만도에 따른 능성어, Epinephelus septemfasciatus의 배란유도 효과

Ovulation Induction Effect of Sevenband Grouper, Epinephelus septemfasciatus in Size and Abdomen Inflation Degree of Mother Fish

  • 투고 : 2016.08.22
  • 심사 : 2016.11.23
  • 발행 : 2016.12.31

초록

능성어, Epinephelus septemfasciatus의 우량 수정란을 생산할 수 있는 어미 선별기준을 설정하기 위하여 해상가두리에서 6년간 키운 시험어의 현태적 특징을 관찰하고 호르몬 처리하여 난질을 관찰 분석하였다. 시험어는 4개의 체중 범위(3.0~4.0, 4.0~5.0, 5.0~6.0, 6.0~7.0 kg)와 뒷지느러미를 기준으로 복부팽만한 정도를 I~IV 단계로 나눈 후 산란빈도, 채란량, 부상률, 수정률, 난발생 생존율 및 부화율을 조사하고, 수정란과 유구의 크기를 측정하였다. 또한 생식소 발달을 확인하기 위하여 복부팽만도 단계별 생식소 중량지수(GSI)를 구하고, 조직학적으로 관찰하였다. 그 결과 어체중이 5.0 kg 이상일 때 모든 개체에서 배란이 유도되었다. 또한 복부팽만도 I, II 단계에서는 배란이 유도되지 않고, III, IV 단계에서만 배란이 유도되었다. 난질은 III 단계가 우수하였고 그 때의 GSI는 $4.68{\pm}0.40%$였다.

We determined the morphologic characteristics (body weight and abdomen inflation degree) of the mother fish of sevenband grouper, Epinephelus septemfasciatus producing the healthy eggs. Experimental fish were chosen from the reared fish in the sea cage for 6 years. The fish were divided into four size groups by body weight: 4.0~5.0, 5.0~6.0, and 6.0~7.0 kg and four stages (I~IV) by the abdomen inflation degree. After hormone treatment, we observed the ovulation amount of induced eggs, rate of buoyant, fertilization, embryonic survival, and hatching. Egg and oil globule diameter was measured. In order to observe gonadal development, we calculated gonadosomatic index (GSI) and conducted its historical analysis. The ovulation occurred from all experimental fishes over 5.0 kg. The rate of buoyant, fertilization, and embryonic survival was the highest in 6.0~7.0 kg. Hatching rate was the highest in 5.0~6.0 kg. Stage I and II did not induce ovulation. GSI was $0.31{\pm}0.10%$ in stage I, $0.74{\pm}0.25%$ in stage II, $4.68{\pm}0.40%$ in stage III and $6.86{\pm}0.12%$ in stage IV. The rate of buoyant, fertilization, embryonic survival and hatching was the highest in stage III.

키워드

참고문헌

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