DOI QR코드

DOI QR Code

Caeoma makinoi에 의한 매실나무 꽃눈녹병의 발생 보고

Flower Bud Chloranthy Disease Caused by Caeoma makinoi on Prunus mume

  • 이승열 (경북대학교 농업생명과학대학) ;
  • 이연희 (경북대학교 농업생명과학대학) ;
  • 박수진 (경북대학교 농업생명과학대학) ;
  • 이한동 (경북대학교 농업생명과학대학) ;
  • 이재진 (경북대학교 농업생명과학대학) ;
  • 강인규 (경북대학교 농업생명과학대학) ;
  • 이향범 (전남대학교 농업생명과학대학) ;
  • 정희영 (경북대학교 농업생명과학대학)
  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Yeon-Hee (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Park, Su-Jin (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Han-Dong (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Jae-Jin (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Hyang Burm (College of Agriculture and Life Sciences, Chonnam National University) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • 투고 : 2016.12.01
  • 심사 : 2016.12.10
  • 발행 : 2016.12.30

초록

2015년 5월, 경상북도 의성군 소재 매실과원에서 비정상적인 엽화증상이 매실나무에서 관찰되었다. 관찰된 엽화증상에서는 외부에 주황색의 돌기가 관찰되었고, 실체현미경으로 관찰한 결과 녹포자퇴로 확인되었다. 자세한 형태를 관찰하기 위해 광학현미경과 주사전자현미경으로 관찰한 결과, 녹포자는 구형, 타원형 또는 난형이었고, 약 $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$ 크기를 가지면서 표면은 무사마귀형으로 관찰되었다. 녹포자의 정확한 동정과 계통학적 유연관계 분석을 위하여, 18S rDNA와 large subunit rDNA 영역을 증폭시킨 후 염기서열을 분석하고 계통학적 유연관계를 분석하였다. 그 결과, 매실나무의 엽화증상에서 관찰된 병원균은 일본에서 분리된 Caeoma makinoi (Teleomorph: B. smilacis) 균주와 100% 일치하는 것으로 확인되었다. 따라서 본 연구에서는 국내 매실나무에서 엽화증상을 일으키는 녹병균을 Caeoma makinoi로 보고하고, 위 병원균에 의한 매실나무 엽화증상을 매실꽃눈녹병으로 제안하고자 한다.

Abnormal chloranthy was observed on Prunus mume trees grown in an orchard in Uiseong, Gyeongbuk province, Korea in 2015. Flower buds showing chloranthy were covered with numerous orange-colored sori, which were confirmed to contain spermogonia and aeciospores by stereo microscopy and light microscopy. When observed using scanning electron microscope, the aeciospores were globose, ellipsoid, or ovoid, and measured $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$, with a verrucae wall ornamentation. To construct a phylogeny, the large and small subunit regions of rDNA were sequenced and compared with those of allied species by the maximum likelihood method. The isolated fungus species showed high similarities with Caeoma makinoi (Teleomorph: Blastospora smilacis), which was previously isolated from P. mume in Japan. Therefore, we initially report this fungal agent parasitic on P. mume as C. makinoi in Korea and name the disease it causes as "flower bud chloranthy disease".

키워드

참고문헌

  1. Hwang JY. Pharmacological effects of Maesil (Prunus mume). Food Sci Ind 2005;38:112-9.
  2. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology;2009.
  3. Kwon JH, Kim WI, Park CS. Occurrence of gray mold on Prunus mume caused by Botrytis cinerea in Korea. Res Plant Dis 2007;13:216-9. https://doi.org/10.5423/RPD.2007.13.3.216
  4. Kim WG, Hong SK, Cho WD, You CH. Brown rot of apricot and mume caused by Phomopsis vexans. Plant Pathol J 2003;19:231-4. https://doi.org/10.5423/PPJ.2003.19.5.231
  5. Jafar H. Studies on the biology of peach rust (Tranzschelia pruni-spinosae Pers.) in New Zealand. N Z J Agric Res 1958;1:642-51. https://doi.org/10.1080/00288233.1958.10431572
  6. Ono Y, Kakishima M, Kudo A, Sato S. Blastospora smilacis, a teleomorph of Caeoma makinoi, and its sorus development. Mycologia 1986;78:253-62. https://doi.org/10.2307/3793171
  7. Ono Y, Kakishima M, Sato S, Harada Y. Blastospora itoana on Smilax nipponica forms a caeoma stage on Prunus grayana in Japan. Mycologia 1987;79:668-73. https://doi.org/10.2307/3807817
  8. Peterson RS, Oehrens E. Mikronegeria alba (Uredinales). Mycologia 1978;70:321-31. https://doi.org/10.2307/3759030
  9. Shaw CG. Rust on Phyllocladus trichomanoides: the first recorded on a member of the podocarpaceae. Trans Br Mycol Soc 1976;67:506-9. https://doi.org/10.1016/S0007-1536(76)80182-9
  10. Aime MC. Toward resolving family-level relationships in rust fungi (Uredinales). Mycoscience 2006;47:112-22. https://doi.org/10.1007/S10267-006-0281-0
  11. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR Protocols: a guide to methods and applications. San Diego:Academic Press; 1990. p. 315-22.
  12. Moncalvo JM, Wang HH, Hseu RS. Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 1995:87;223-38. https://doi.org/10.2307/3760908
  13. O'Donnell K. Fusarium and its near relatives. In: Reynolds DR, Taylor JW, editors. The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. Wallingford: CAB International; 1993. p. 225-33.
  14. Park CS. Research report no. 1: fungus disease of plants in Korea. Daejeon: College of Agriculture, Chungnam National University; 1958.
  15. Kusano S. Notes on the Japanese fungi: IV. Caeoma on Prunus. Bot Mag Tokyo 1906;20:47-51.
  16. Imamura S, Saito Y. A disease of plum by Caeoma makinoi heavily occurred in Ina region in Nagano Prefecture, Japan. Plant Prot 1975;29:481-3.
  17. Hernandez JR, Hennen JF. Rust fungi causing galls, witches' brooms, and other abnormal plant growths in northwestern Argentina. Mycologia 2003:95;728-55. https://doi.org/10.1080/15572536.2004.11833076
  18. Kim KH, Seo ST, Oh ES, Lee SH, Lee SK, Shin SC. Disease of conifers in Korea. Seoul: National Institute of Forest Science;2007.
  19. The Korean Society of Mycology. National list of species of Korea: Basidiomycota. Incheon: National Institute of Biological Resources; 2015.