The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Genotyping of the Wild Mushroom Lentinula edodes from Mt. Jungwang and Mt. Gariwang in Korea

  • Jeong, Yeun Sug (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Jang, Yeongseon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Ryoo, Rhim (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Kim, Ki-Hwan (C&K Genomics) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science)
  • Received : 2016.09.17
  • Accepted : 2016.11.08
  • Published : 2016.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Lentinula edodes is an edible wild mushroom that can be found in mountainous regions of the Korean peninsula. Wild oak mushrooms were collected from Quercus mongolica at an elevation of more than 1,000 m on Mt. Jungwang and Mt. Gariwang in Gangwon province. We examined 10 oak mushroom strains to evaluate the genetic similarity among strains. Genetic similarity was determined based on the analysis of microsatellite markers (Led A2, Led A8, Led B2, Led B6, and Led D6) registered in the National Center for Biotechnology Information. We also performed dual culture tests on potato dextrose agar for 2 months at $25^{\circ}C$. The observed heterozygosity across all microsatellites ranged from 0.00 and 0.60 among 5 microsatellite markers, and the polymorphism information content values of Led A2, Led A8, Led B2, Led B6, and Led D6 were 0.0000, 0.8144, 0.6194, 0.4892, and 0.5702, respectively (mean value = 0.4987). Confrontation lines between strains were formed for almost all combinations. In conclusion, the oak mushroom populations of Mt. Jungwang and Mt. Gariwang have mixed gene pools. However, further studies are needed to identify genetic similarities and variations among these populations.

Keywords

References

  1. Kim KH, Kim YY, Ka KH, Lee HS, Bak WC, Jeong SJ, Seong JY, Suh DS. Microsatellite markers for population-genetic studies of shiitake (Lentinula edodes) strains. Genes Genomics 2009;31:421-30. https://doi.org/10.1007/BF03191855
  2. Xiao Y, Liu W, Dai Y, Fu C, Bian Y. Using SSR markers to evaluate the genetic diversity of Lentinula edodes natural germplasm in China. World J Microbiol Biotechnol 2010;26:527-36. https://doi.org/10.1007/s11274-009-0202-4
  3. Liu J, Wang ZR, Li C, Bian YB, Xiao Y. Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers. J Basic Microbiol 2015;55:749-60. https://doi.org/10.1002/jobm.201400774
  4. Qin LH, Tan Q, Chen MJ, Pan YJ. Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes. FEMS Microbiol Lett 2006;257:112-6. https://doi.org/10.1111/j.1574-6968.2006.00145.x
  5. Karaoglu H, Lee CM, Meyer W. Survey of simple sequence repeats in completed fungal genomes. Mol Biol Evol 2005;22:639-49. https://doi.org/10.1093/molbev/msi057
  6. Terashima K, Matsumoto T, Hasebe K, Fukumasa-Nakai Y. Genetic diversity and strain-typing in cultivated strains of Lentinula edodes (the shiitake mushroom) in Japan by AFLP analysis. Mycol Res 2002;106:34-9. https://doi.org/10.1017/S0953756201005007
  7. Terashima K, Matsumoto T. Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heatdried fruiting body. Mycoscience 2004;45:79-82. https://doi.org/10.1007/S10267-003-0152-X
  8. Liu J. Power Marker v3.0 manual [Internet]. Power marker;2016. Available from: http://statgen.ncsu.edu/powermarker/downloads/Manual.pdf.
  9. Liu K, Muse SV. PowerMarker: integrated analysis environment for genetic marker data. Bioinformatics 2005;21:2128-9. https://doi.org/10.1093/bioinformatics/bti282
  10. Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978;89:583-90.
  11. Xiang X, Li C, Li L, Bian Y, Kwan HS, Nong W, Cheung MK, Xiao Y. Genetic diversity and population structure of Chinese Lentinula edodes revealed by InDel and SSR markers. Mycol Prog 2016;15:37. https://doi.org/10.1007/s11557-016-1183-y
  12. Chiu SW, Wang ZM, Chiu WT, Lin FC, Moore D. An integrated study of individualism in Lentinula edodes in nature and its implication for cultivation strategy. Mycol Res 1999;103:651-60. https://doi.org/10.1017/S095375629900859X
  13. Li AZ, Xu XF, Lin FX, Cheng SM, Lin FC. Cloning and identification of partial DNA fragment for the B mating-type factor in Lentinula edodes using degenerate PCR. World J Microbiol Biotechnol 2007;23:411-5. https://doi.org/10.1007/s11274-006-9239-9
  14. Rice WR. Analyzing tables of statistical tests. Evolution 1989;43:223-5. https://doi.org/10.2307/2409177