DOI QR코드

DOI QR Code

New Records of Three Aquatic Fungi Isolated from Freshwater in Samcheok and Yeongju, Korea

강원 삼척 및 경북 영주의 담수지역에서 발굴된 수생균류 3종의 국내 최초 보고

  • Mun, Hye Yeon (Fungal Resources Research Division, Freshwater Bioresources Research Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Goh, Jaeduk (Fungal Resources Research Division, Freshwater Bioresources Research Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Oh, Yoosun (Fungal Resources Research Division, Freshwater Bioresources Research Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Chung, Namil (Fungal Resources Research Division, Freshwater Bioresources Research Bureau, Nakdonggang National Institute of Biological Resources)
  • 문혜연 (국립낙동강국립생물자원관 담수생물조사연구실 균류자원조사부) ;
  • 고재덕 (국립낙동강국립생물자원관 담수생물조사연구실 균류자원조사부) ;
  • 오유선 (국립낙동강국립생물자원관 담수생물조사연구실 균류자원조사부) ;
  • 정남일 (국립낙동강국립생물자원관 담수생물조사연구실 균류자원조사부)
  • Received : 2016.11.25
  • Accepted : 2016.12.08
  • Published : 2016.12.30

Abstract

Three aquatic fungi were isolated from samples of freshwater-deposited plant litter and foam collected in Samcheok, Gangwon-do, and Yeongju, Gyeongsangbuk-do, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS) rDNA region, the three isolates NNIBRFG329, NNIBRFG339, and NNIBRFG19 were confirmed as aquatic fungi: Articulospora tetracladia, Margaritispora aquatica, and Aquanectria penicillioides. These species were known as aquatic fungi but neither species has been previously reported in Korea.

본 연구에서는 강원 삼척 및 경북 영주 지역의 하천에서 채집해 온 담수 침전식물체 및 담수 포말에서 균을 분리하여 수생균류 3종을 발굴하였다. 분리된 균을 형태학적 특징 관찰과 internal transcribed spacers rDNA 유전자 분석을 통해 동정한 결과, NNIBRFG329, NNIBRFG339, NNIBRFG19 균주는 각각 Articulospora tetracladia, Margaritispora aquatica, Aquanectria penicillioides이었다. 이들 3종의 균류는 불완전균강에 속하는 담수 서식 균류로 국내 미기록종으로 보고하는 바이다.

Keywords

References

  1. Ingold CT. Aquatic hyphomycetes of decaying alder leaves. Trans Br Mycol Soc 1942;25:339-417. https://doi.org/10.1016/S0007-1536(42)80001-7
  2. Ferreira V, Encalada AC, Graca MA. Effects of litter diversity on decomposition and biological colonization of submerged litter in temperate and tropical streams. Freshw Sci 2012;31:945-62. https://doi.org/10.1899/11-062.1
  3. Ferreira V, Goncalves AL, Pratas J, Canhoto C. Uranium adsorption by Articulospora tetracladia: can aquatic hyphomycetes be natural bioremediators of uranium contaminated streams? In: Mendez-Vilas A, editor. Microorganisms in industry and environment: From scientific and industrial research to consumer products. London: World Scientific; 2011. p. 265-9.
  4. Baschien C, Tsui CK, Gulis V, Szewzyk U, Marvanova L. The molecular phylogeny of aquatic hyphomycetes with affinity to the Leotiomycetes. Fungal Biol 2013;117:660-72. https://doi.org/10.1016/j.funbio.2013.07.004
  5. Chauvet E, Suberkropp K. Temperature and sporulation of aquatic hyphomycetes. Appl Environ Microbiol 1998;64:1522-5.
  6. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30.
  7. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947-8. https://doi.org/10.1093/bioinformatics/btm404
  8. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  10. Abdullah SK, Descals E, Webster J. Teleomorphs of three aquatic hyphomycetes. Trans Br Mycol Soc 1981;77:475-83. https://doi.org/10.1016/S0007-1536(81)80094-0
  11. Baral HO, Krieglsteiner GJ. Bausteine zu einer Askomyzeten- Flora der Bundesrepublik Deutschland: In Suddeutschland gefundene Inoperculate Discomyzeten: mit taxonomischen, okologischen, chorologischen Hinweisen. Verbreitung und Okologie ausgewahlter Nichtblätterpilze in der Bundesrepublik Deutschland (Mitteleuropa). Tubingen: Dt. Ges. fur Mykologie; 1985.
  12. Fungal Barcoding Database. Identification; Pairwise sequence alignment [Internet]. Utrecht, The Netherlands: CBS-KNAW Fungal Biodiversity Centre; 2011 [cited 2016 Nov 4]. Available from: http://www.fungalbarcoding.org/BioloMICSSequences.aspx?file=all.
  13. Ingold CT. Some new aquatic hyphomycetes. Trans Br Mycol Soc 1944;27:35-47. https://doi.org/10.1016/S0007-1536(44)80006-7
  14. Lombard L, van der Merwe NA, Groenewald JZ, Crous PW. Generic concepts in Nectriaceae. Stud Mycol 2015;80:189-245. https://doi.org/10.1016/j.simyco.2014.12.002