Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Discovery of Deleterious nsSNPs on the Genes related to the Lipid Metabolism and Prediction of Changes on Biological Function in Korean Native Chicken

한국 재래닭에서 지질대사 관련 유전자에 존재하는 유해성 nsSNP 발굴 및 생물학적 기능 예측

  • Oh, Jae-Don (Department of Animal Biotechnology, Chonbuk National University) ;
  • Shin, Dong-Hyun (Department of Animal Biotechnology, Chonbuk National University) ;
  • Shin, Sang-Soo (Department of Animal Biotechnology, Kyungpook National University) ;
  • Yoon, Chang (Department of Animal Biotechnology, Chonbuk National University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National University)
  • 오재돈 (전북대학교 동물생명공학과) ;
  • 신동현 (전북대학교 동물생명공학과) ;
  • 신상수 (경북대학교 축산생명공학과) ;
  • 윤창 (전북대학교 동물생명공학과) ;
  • 송기덕 (전북대학교 동물생명공학과)
  • Received : 2016.12.07
  • Accepted : 2016.12.26
  • Published : 2016.12.31
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Abstract

In this study, we aimed to identify the nonsynonymous single nucleotide polymorphisms (nsSNPs) located in lipid metabolism-related genes because lipids are an important factor affecting the taste and flavor of meat, and they predict the functional consequences. The results showed that we identified 139 common nsSNPs in all five Korean native chicken (KNC) lines from the 81 genes related to lipid metabolism. Furthermore, sorting intolerant from tolerant (SIFT) and polymorphism phenotyping v2 (Polyphen-2) analyses predicted that among the genes, 14 nsSNPs of nine genes might be deleterious. Protein domain prediction of the nine genes revealed that all deleterious nsSNPs identified in this study were located outside the functional domain. This observation suggests that the common deleterious nsSNPs might be dispensable and have a minor effect on the traits of the KNCs.

한국 재래닭은 맛이나 기호성에서 한국 소비자들의 입맛에 적합한 것으로 알려져 있는데, 이러한 한국 재래닭의 특성을 결정짓는 유전적 요소들이 어떤 것들이 있는지 많이 밝혀지지 않았다. 본 연구에서는, 맛을 결정하는 요인 중 하나인 지질의 대사와 관련된 유전자들 내에 존재하는 nsSNP 연구를 통해서, 한국 재래닭의 유전적 특성을 설명하기 위한 자료를 확보하고자 분석을 실시하였다. 지질대사와 관련한 81개 유전자에 대하여 한국 재래닭에 공통으로 존재하는 nsSNP를 139개 동정하였으며, 이 중 9개의 유전자에 존재하는 14개의 nsSNP가 유해성 nsSNP로 확인되었다. 이들 9개 유전자에 대해 단백질 도메인 예측을 실시하였으며, 그 결과, 유해성 nsSNP들에 의해 바뀐 아미노산들이 모두 주요도메인의 외부에 존재함을 알 수 있었다. 이는 한국 재래닭에서 공통으로 발견한 nsSNP들이 유전자의 고유 기능에 큰 영향을 미치기 보다는 다소 미미한 변화를 통해 한국 재래닭 고유의 특성을 형성하는데 더 큰 역할을 한 것으로 사료된다. 이러한 부분들은 차후 실험을 통해 밝혀져야 할 부분이며, 한국 재래닭의 맛과 관련된 형질 개량을 위한 분자육종 기술 개발에 주요한 자료로 활용될 수 있을 것으로 기대되어진다.

Keywords

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