Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Detection of LM canola with tolerance to glyphosate and glufosinate-ammonium via the Environmental monitoring in South Korea

자연생태계 모니터링을 통한 glyphosate와 glufosinate-ammonium에 저항성을 가지는 유전자변형 캐놀라의 발견

  • Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Jo, Beom-Ho (Bio-industrial Team, Bio Center, Chungnam Techno Park) ;
  • Moon, Jeong Chan (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Lee, Jung Ro (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Choi, Wonkyun (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Seol, Min-A (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Kim, Mi-Jeong (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Song, Hae-Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE))
  • 신수영 (국립생태원 생태보전연구실) ;
  • 조범호 (충남테크노파크 바이오센터 생물산업팀) ;
  • 문정찬 (국립생태원 생태보전연구실) ;
  • 이중로 (국립생태원 생태보전연구실) ;
  • 최원균 (국립생태원 생태보전연구실) ;
  • 설민아 (국립생태원 생태보전연구실) ;
  • 김미정 (국립생태원 생태보전연구실) ;
  • 송해룡 (국립생태원 생태보전연구실)
  • Received : 2016.11.09
  • Accepted : 2016.12.21
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Living modified (LM) crops are imported each year to South Korea as food and feeds, LM canola being one of the imported crops. The cultivation of LM crops is not permitted in South Korea but the import of these crops is increasing. In this study, we surveyed the environmental risk of imported LM canola at 9 provinces, from March 2009 to June 2013. Monitoring of canola was conducted around feed factories, roadsides, harbors, farmhouses, and flower festival regions. From the total of 595 canola samples collected from 1850 monitoring sites, we identified 6 LM canola samples. The LM canola samples were subjected to protein and DNA based analysis. PCR analyses using approved 5 single event primers (T45, MS8, RT73, Rf3 and Topas 19-2) revealed that two crops were glyphosate-resistant LM canolas, and four were glufosinate-resistant LM canolas. This study suggested that environmental monitoring is a useful research tool to manage LM crops unintentionally introduced into the environment in South Korea. This result can be used as a basis for future post-management of canola crops.

식품용 및 사료용 유전자변형작물은 매년 국내에 수입이 되고 있으며 유전자변형 캐놀라는 중요한 수입 작물 중의 하나이다. 유전자변형작물의 국내 재배는 허용되고 있지않지만 수입양은 매년 증가하고 있는 실정이다. 본 연구에서는 2009년부터 2013년까지 전국 9개 도를 대상으로 국내 수입된 유전자변형 캐놀라의 자연생태계 내 유출 정도를 파악하고자 모니터링을 실시하였다. 모니터링 조사는 항만, 사료공장, 축산농가, 운송로, 축제지 주변을 대상으로 실시하였다. 채집된 유전자변형 캐놀라 의심시료는 DNA에 기반을 둔 방법과 단백질에 기반을 둔 방법으로 분석하였다. 총 1850개 지점 조사에서 총 595개의 의심시료를 발견하였으며 6개의 유전자변형 캐놀라를 확인하였다. 국내 수입 승인된 단일이벤트 T45, MS8, RT73, Rf3, Topas 19-2의 primer를 사용한 PCR반응을 통해 2개의 glyphosate 저항성을 가지는 이벤트와 4 개의 glufosinate-ammonium에 저항성을 가지는 이벤트를 확인하였다. 본 연구를 통해 자연환경 모니터링 연구가 자연생태계 내의 유전자변형작물의 비의도적 유출을 예방하기 위해 매우 유용하다는 것을 알 수 있으며 사후관리를 수행하기 위한 기초자료를 구축하는데 활용될 것으로 사료된다.

Keywords

References

  1. Baek HJ, Won SY, Kim JK, Shon SI, Lee K, Cho MR, Song JK, Yoon MS, Lee J, Jin YM, Ryu TH. (2008) Study on the gene introgression from GM chinese cabbage to major crops in cruciferae. Korean J Int Agri 20:124-129
  2. Charles DC, Bogni A, Mazzara M, Savini C, Van Den EG (2006) Event-specific method for the quantification of oilseed rape line T45 using real-time PCR - validation report and protocol - sampling and DNA extraction of oilseed rape. EUR 22357 EN.
  3. European Commission, Joint Research Centre (JRC). Available from: http://gmo-crl.jrc.ec.europa.eu
  4. Hoyle M, Hayter K, Cresswell JE. (2007) Effect of pollinator abundance on self-fertilization and gene flow: application to GM canola. Ecological Applications 17:2123-2135 https://doi.org/10.1890/06-1972.1
  5. James C (2015) Global status of commercialized biotech/GM crops: 2015. ISAAA Brief. No. 50.
  6. Jo BH, Lee JR, Choi W, Moon JC, Shin SY, Eum SJ, Seol MA, Kim IR, Song HR. (2015) Development of multiplex PCR-based detection method for five approved LM canola events in Korea J Plant Biotechnol 42:117-122 https://doi.org/10.5010/JPB.2015.42.2.117
  7. Kim JH, Kim YR, Kim HY (2011) Current status on the development of detection methods for genetically modified plants J Plant Biotechnol 38:143-150 https://doi.org/10.5010/JPB.2011.38.2.143
  8. KBCH: Korea Biosafety Clearing House. [Internet]. 2015. Status of risk assessment of GMO in korea. Available from: http://www.biosafety.or.kr.
  9. Lee BK, Kim CG, Park JY, Yi HB, Park KW, Jeong SC, Yoon WK, An JH, Cho KH, Kim HM (2007) Survey of herbicide resistant oilseed rapes around the basin of rivers in incheon harbor area. Kor J Weed Sci 27(1):29-35
  10. Lim Y, Yook MJ, Zhang CJ, Nah G, Park S, Kim DS. (2015) Dormancy associated weedy risk of the F1 hybrid resulted from gene flow from oilseed rape to mustard. Weed Turf Sci 4:35-43 https://doi.org/10.5660/WTS.2015.4.1.35
  11. Mazzara M, Bogni A, Savini C, Van Den EG (2007) event-specific method for the quantification of oilseed rape line Ms8 using real-time PCR - validation report and protocol- seeds sampling and DNA extraction of oilseed rape. EUR 22917 EN.
  12. Mazzara M, Grazioli E, Savini C, Van Den EG (2007) Event-specific method for the quantification of oilseed tape Line RT73 using teal-time PCR - validation report and protocol - seeds sampling and DNA extraction of oilseed rape. EUR 22918 EN.
  13. Mazzara M (2011) In-house validation of an event-specific method for the quantification of oilseed rape Topas 19/2 using real-time PCR - validation report and protocol. CRLVL12/04VP
  14. Park KW, Kim CG, Lee BK, Kim DY, Park JY, Kim DI, Kwon MC, Yi HB, Kim HM (2007) Monitoring of imported genetically modified crops in the cultivated fields in korea. Kor J Weed Sci 27(4):318-324
  15. Savini C, Bogni A, Mazzara M, Van Den EG (2013) Event-specific method for the quantification of oilseed rape line Rf3 using real-time PCR(V.1.01) - validation report and protocol - seeds sampling and DNA extraction. EUR 26149 EN.
  16. Sole R (2015) Bioengineering the biosphere. Ecol Complex 22:40-49 https://doi.org/10.1016/j.ecocom.2015.01.005
  17. Susarla S, Medina VF, McCutcheon SC. (2002) Phytoremediation: an ecological solution to organic chemical contamination. Ecol Eng 18:647-65 https://doi.org/10.1016/S0925-8574(02)00026-5

Cited by

  1. L.) in Gyeonggi-do of South Korea in 2014 vol.45, pp.1, 2018, https://doi.org/10.5010/JPB.2018.45.1.077