Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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A simple phenotyping method for deep-rooting rice grown in pots

  • Han, Jae-Hyuk (Department of Plant Life & Environmental Science, HanKyong National University) ;
  • Shin, Na-Hyun (Department of Plant Life & Environmental Science, HanKyong National University) ;
  • Moon, Jae-Hoon (Department of Plant Life & Environmental Science, HanKyong National University) ;
  • Chin, Joong Hyoun (Graduate School of Integrated Bioindustry, Sejong University) ;
  • Yoo, Soo-Cheul (Department of Plant Life & Environmental Science, HanKyong National University, Institute of Ecological Phytochemistry, Hankyong National University)
  • Received : 2016.12.06
  • Accepted : 2016.12.26
  • Published : 2016.12.31
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Abstract

Deep rooting, which enables plants to extract water from greater soil depths, is a critical strategy for improving plant survival under water-deficient conditions. However, as it is difficult to observe intact root systems belowground, several techniques have been developed to screen deep- and shallow-rooting phenotypes in rice. Here, we introduce a simple and convenient method for deep- and shallow-rooting phenotyping using a unique combination of sand, soil, and plastic mesh netting. Vandana, a drought-tolerant rice variety, and Dongjin, a Korean japonica rice variety, were used to analyze root phenotypes. No significant differences in root length were observed in rice grown under irrigated conditions regardless of net position, whereas roots were significantly longer, and ratio of deep root (RDR) values were significantly higher in Vandana rice grown under semi-drought conditions. In summary, this simple and useful method represents a low-cost means of phenotyping the roots of rice and other crops grown in various-sized pots and at multiple plant growth stages.

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References

  1. Abe J, Morita S (1994) Growth direction of nodal roots in rice: its variation and contribution to root system formation. Plant Soil, 165(2):333-337 https://doi.org/10.1007/BF00008078
  2. Almeida DM, Almadanim MC, Lourenco T, Abreu IA, Saibo NJ, Oliveira MM (2016) Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought. Environmental Responses in Plants: Methods and Protocols, 155-182
  3. Araki H, Morita S, Tatsumi J, Iijima M (2002) Physiol-morphological analysis on axile root growth in upland rice. Plant Prod Sci, 5(4):286-293 https://doi.org/10.1626/pps.5.286
  4. Angus JF, Hasegawa S, Hsiao TC, Liboon SP, Zandstra HG (1983) The water balance of post-monsoonal dryland crops. J Agric Sci, 101(03):699-710 https://doi.org/10.1017/S0021859600038739
  5. Biswas J, Chowdhury B, Bhattacharya A, Mandal AB (2002) In vitro screening for increased drought tolerance in rice. In Vitro Cellular & Developmental Biology-Plant, 38(5):525-530 https://doi.org/10.1079/IVP2002342
  6. Blum A, Mayer J, Golan G (1989) Agronomic and physiological assessments of genotypic variation for drought resistance in sorghum. Crop Pasture Sci, 40(1):49-61 https://doi.org/10.1071/AR9890049
  7. Clark RT, MacCurdy RB, Jung JK, Shaff JE, McCouch SR (2011) Three-dimensional root phenotyping with a novel imaging and software platform. Plant Physiol, 156(2):455-465 https://doi.org/10.1104/pp.110.169102
  8. Fukai S, Cooper M (1995) Development of drought-resistant cultivars using physiomorphological traits in rice. Field Crops Res, 40(2):67-86 https://doi.org/10.1016/0378-4290(94)00096-U
  9. Fukai S, Inthapan P (1988) Growth and yield of rice cultivars under sprinkler irrigation in south-eastern Queensland. 3. Water extraction and plant water relations dash comparison with maize and grain sorghum. Anim Prod Sci, 28(2):249-252 https://doi.org/10.1071/EA9880249
  10. Gowda VR, Henry A, Yamauchi A, Shashidhar HE, Serraj R (2011) Root biology and genetic improvement for drought avoidance in rice. Field Crops Res, 122(1):1-13 https://doi.org/10.1016/j.fcr.2011.03.001
  11. Kato Y, Abe J, Kamoshita A, Yamagishi J (2006) Genotypic variation in root growth angle in rice (Oryza sativa L.) and its association with deep root development in upland fields with different water regimes. Plant Soil, 287(1-2):117-129 https://doi.org/10.1007/s11104-006-9008-4
  12. Levitt J, Levitt J (1972) Responses of plants to environmental stresses. New York: Academic press
  13. Lynch JP (2013) Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems. Ann Bot, 112(2):347-357 https://doi.org/10.1093/aob/mcs293
  14. O'Toole JC (1979) Drought resistance in cereals: rice, a case study
  15. Oyanagi A, Nakamoto T, Wada M (1993) Relationship between root growth angle of seedlings and vertical distribution of roots in the field in wheat cultivars. Jpn. J. Crop. Sci. 62:565-570 https://doi.org/10.1626/jcs.62.565
  16. Samson BK, Hasan M, Wade LJ (2002) Penetration of hardpans by rice lines in the rainfed lowlands. Field Crops Res, 76(2):175-188 https://doi.org/10.1016/S0378-4290(02)00038-2
  17. Shashidhar HE (2012) Methodologies for root drought studies in rice. (eds) Int Rice Res Inst
  18. Trachsel S, Kaeppler SM, Brown KM, Lynch JP (2013) Maize root growth angles become steeper under low N conditions. Field Crops Res, 140:18-31 https://doi.org/10.1016/j.fcr.2012.09.010
  19. Uga Y, Okuno K, Yano M (2011) Dro1, a major QTL involved in deep rooting of rice under upland field conditions. J Exp Bot, 62(8):2485-2494 https://doi.org/10.1093/jxb/erq429
  20. Uga Y, Sugimoto K, Ogawa S, Rane J, Ishitani M, Hara N, Inoue H (2013) Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. Nature Genet, 45(9):1097-1102 https://doi.org/10.1038/ng.2725
  21. Uga Y, Yamamoto E, Kanno N, Kawai S, Mizubayashi T, Fukuoka S (2013) A major QTL controlling deep rooting on rice chromosome 4. Sci Rep, 3:3040 https://doi.org/10.1038/srep03040
  22. Venuprasad R, Lafitte HR, Atlin GN (2007) Response to direct selection for grain yield under drought stress in rice. Crop Sci, 47(1):285-293 https://doi.org/10.2135/cropsci2006.03.0181
  23. Wang H, Yamauchi A (2006) Growth and function of roots under abiotic stress in soils. In B. Huang (eds) Plant-Environment Interactions, Third Edition (pp. 271-319). CRC Press
  24. Yoshida S, Hasegawa S (1982) The rice root system: its development and function. Drought resistance in crops with emphasis on rice, Los Banos, Philippines: Int Rice Res Inst, 97-114