Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effects of Carbenoxolone and P2X recepter antagonist combined therapy on oral neuropathic pain in rat

신경병증 통증 모델에서 Carbenoxolone과 P2x receptor 길항제의 효과

  • Received : 2015.11.09
  • Accepted : 2016.02.04
  • Published : 2016.02.29
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Abstract

The study was conducted to confirm the analgesic effects of the Carbenoxolone(CBX)and P2X receptor antagonist(iso-PPADS), which separates the gap junction in the facial neuropathic pain model. The experiment used white male Sprague-Dawley rats (240~280g). The second left molars on the lower jaw was extracted to induce facial neuropathic pain, and small dental implants were implanted to induce damage to the inferior alveolar nerve. When CBX was injected twice daily to the abdominal cavity, a significant analgesic effect at 5ug/kg was observed(p<0.05). In addition, when iso-PPADS was injected twice daily into the abdominal cavity, a significant analgesic reaction was observed at $25{\mu}g/kg$(p<0.05). When the two drugs were injected together at a low concentration, in which they did not display an effect, they displayed a significant analgesic reaction at CBX 1ug/kg and iso-PPADS 2.5ug/kg(p<0.05). When a gap injunction block using a low concentration of CBX and a low concentration P2X receptor antagonist was injected together, the pain suppressing effect was observed against the orofacial neuropathic pain mechanism. These results make it possible to determine that the gap junction block using CBX and the injection of the P2X receptor antagonist plays an important role in the pain management of the facial region.

본 연구는 안면부 신경병증 통증 모델에서 간극결합을 분리하는 Carbenoxolone(CBX)과 P2X 수용체의 길항제(iso-PPADS)의 진통작용을 확인하고자 수행되었다. 실험동물은 Sprague-Dawley계 수컷 흰쥐(240~280g)를 사용하였으며, 안면부 신경병증 통증을 유발하기 위해 흰쥐의 하악 왼쪽 두 번째 어금니를 발거하고, 아랫 이틀신경의 손상을 유도하기 위해 소형 치과용 임플란트를 식립하였다. CBX를 복강으로 하루 2번 투여 했을 때, 25ug/kg에서 유의한 진통반응이 나타났다(p<0.05). iso-PPADS 역시 복강으로 하루 2번 투여 했을 때, 25ug/kg에서 유의한 진통반응이 나타났다(p<0.05). 두 약물의 각각의 효과가 나타나지 않은 저 농도에서 함께 투여하였을 때, CBX 1ug/kg, iso-PPADS 2.5ug/kg를 투여 시 유의한 진통 효과가 나타났다(p<0.05). 저 농도의 CBX를 이용한 간극결합의 차단과 저 농도의 P2X 수용체의 길항제를 함께 투여 시 구강 안면신경병증통증기전에서 통증을 억제하는 효과를 확인할 수 있었다. 이러한 결과를 통하여 CBX를 이용한 간극결합의 차단과 P2X 수용체의 길항제의 투여가 안면부 통증조절에 중요한 역할을 담당할 것으로 사료된다.

Keywords

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