Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Nationwide survey of Turnip mosaic virus and selection of cabbage lines with resistance against major TuMV isolates

  • Chung, Jinsoo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Han, Jae-yeong (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jungkyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Ju, Hyekyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Gong, Junsu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eun-young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Su Ryun (Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lim, Yong Pyo (Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hammond, John (United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2016.10.24
  • Accepted : 2016.11.28
  • Published : 2016.12.31
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Abstract

This survey was conducted in 2015, following up on theed tthe occurrence of Turnip mosaic virus (TuMV) nationwide in radish and Chinese cabbage fields of 28 cities in South Korea. A total of 152 samples of Raphanus sativus and 29 samples of Brassica rapa, showing virus-like symptoms, were collected. Among these, 107 B. rapa samples and 9 B. rapa samples were positive for TuMV when analyzed by RT-PCR. The TuMV strains found in the two crops showed 99% homology in nucleotide and amino acid sequences of coat protein to each other. Furthermore, their sequences showed 99% homology to the sequences of TuMV isolates R007 (GenBank: KU140420) and R041 (GenBank: KU140421) that were collected in 2014. These results suggested TuMV isolated from radish and cabbage in 2015 were the same strain as the isolates R007 and R041 collected in 2014. A screening test was conducted using these two isolates to select TuMV-resistant B. rapa lines out of 167 B. rapa breeding lines.and identified eight lines resistant to R007 (Kenshin, 279002, 279012, 279064, 279081, MP, C-21, HKC-004) and nine lines resistant to R041 (C-26, HKC-005, 11Su-4, 11Su-5, 11Su-7, 11Su-8, Tian Jin Lv Qing Ma Ye, CNU_141193, Jing Lv 60). Our prior data indicated 4.24% difference in sequences between the two isolates and these can serve as potential tools to develop B. rapa markers to screen for resistance against TuMV strainsin breeding populations.

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References

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