Journal of the Korean institute of surface engineering (한국표면공학회지)

The Korean Institute of Surface Engineering (한국표면공학회)
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Micropatterning on Biodegradable Nanofiber Scaffolds by Femtosecond Laser Ablation Process

펨토초 레이저 절삭 공정을 이용한 생분해성 나노섬유 표면 미세 패터닝 공정

  • Chung, Yongwoo (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology) ;
  • Jun, Indong (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology) ;
  • Kim, Yu-Chan (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology) ;
  • Seok, Hyun-Kwang (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology) ;
  • Chung, Seok (School of Mechanical Engineering, Korea University) ;
  • Jeon, Hojeong (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology)
  • 정용우 (한국과학기술연구원 생체재료연구단) ;
  • 전인동 (한국과학기술연구원 생체재료연구단) ;
  • 김유찬 (한국과학기술연구원 생체재료연구단) ;
  • 석현광 (한국과학기술연구원 생체재료연구단) ;
  • 정석 (고려대학교 마이크로나노시스템) ;
  • 전호정 (한국과학기술연구원 생체재료연구단)
  • Received : 2016.11.01
  • Accepted : 2016.12.13
  • Published : 2016.12.31
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Abstract

A biodegradable nanofiber scaffolds using electrospining provide fibrous guidance cues for controlling cell fate that mimic the native extracellular matrix (ECM). It can create a pattern using conventional electrospining method, but has a difficulty to generate one or more pattern structures. Femtosecond(fs) laser ablation has much interested in patterning on biomaterials in order to distinguish the fundamental or systemic interaction between cell and material surface. The ablated materials with a short pulse duration using femtosecond laser that allows for precise removal of materials without transition of the inherent material properties. In this study, linear grooves and circular craters were fabricated on electrospun nanofiber scaffolds (poly-L-lactide(PLLA)) by femtosecond laser patterning processes. As parametric studies, pulse energy and beam spot size were varied to determine the effects of the laser pulse on groove size. We confirmed controlling pulse energy to $5{\mu}J-20{\mu}J$ and variation of lens maginfication of 2X, 5X, 10X, 20X created grooves of width to approximately $5{\mu}m-50{\mu}m$. Our results demonstrate that femtosecond laser processing is an effective means for flexibly structuring the surface of electrospun PLLA nanofibers.

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References

  1. Watt, Fiona M., and Wilhelm TS Huck. Role of the extracellular matrix in regulating stem cell fate. Nature reviews Molecular cell biology 14.8 (2013) 467-473. https://doi.org/10.1038/nrm3620
  2. Trappmann, Britta, et al. Extracellular-matrix tethering regulates stem-cell fate. Nature materials 11.7 (2012) 642-649. https://doi.org/10.1038/nmat3339
  3. Place, Elsie S., Nicholas D. Evans, and Molly M. Stevens. Complexity in biomaterials for tissue engineering. Nature materials 8.6 (2009) 457-470. https://doi.org/10.1038/nmat2441
  4. Wang, Xianfeng, Bin Ding, and Bingyun Li. Biomimetic electrospun nanofibrous structures for tissue engineering. Materials today 16.6 (2013) 229-241. https://doi.org/10.1016/j.mattod.2013.06.005
  5. Jiang, Tao, et al. Electrospinning of polymer nanofibers for tissue regeneration. Progress in Polymer Science 46 (2015) 1-24. https://doi.org/10.1016/j.progpolymsci.2014.12.001
  6. Doshi, Jayesh, and Darrell H. Reneker. Electrospinning process and applications of electrospun fibers. Industry Applications Society Annual Meeting, (1993).
  7. Murugan, Ramalingam, and Seeram Ramakrishna. Design strategies of tissue engineering scaffolds with controlled fiber orientation. Tissue engineering 13.8 (2007) 1845-1866. https://doi.org/10.1089/ten.2006.0078
  8. Grigoropoulos, Costas P. Transport in laser microfabrication: fundamentals and applications. Cambridge University Press, (2009) 176-180.
  9. Chichkov, Boris N., et al. Femtosecond, picosecond and nanosecond laser ablation of solids. Applied Physics A 63.2 (1996) 109-115. https://doi.org/10.1007/BF01567637
  10. Higgins, Daniel A., et al. High-resolution direct-write multiphoton photolithography in poly (methylmethacrylate) films. Applied physics letters 88.18 (2006) 184101. https://doi.org/10.1063/1.2200476
  11. Korte, Frank, et al. Towards nanostructuring with femtosecond laser pulses. Applied Physics A 77.2 (2003) 229-235. https://doi.org/10.1007/s00339-003-2110-z
  12. Hartmann, N., et al. Subwavelength patterning of alkylsiloxane monolayers via nonlinear processing with single femtosecond laser pulses. Applied Physics Letters 92.22 (2008) 3111.
  13. Hwang, David J., Costas P. Grigoropoulos, and Tae Y. Choi. Efficiency of silicon micromachining by femtosecond laser pulses in ambient air. Journal of applied physics 99.8 (2006) 083101. https://doi.org/10.1063/1.2187196
  14. Woon Choi, Hae, et al. Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation. Journal of Laser Applications 19.4 (2007) 225-231. https://doi.org/10.2351/1.2795749
  15. Lee, Benjamin Li-Ping, et al. Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds. Acta biomaterialia 8.7 (2012) 2648-2658. https://doi.org/10.1016/j.actbio.2012.04.023
  16. Lim, Yong Chae, et al. Micropatterning and characterization of electrospun poly ($\varepsilon$-caprolactone)/gelatin nanofiber tissue scaffolds by femtosecond laser ablation for tissue engineering applications. Biotechnology and bioengineering 108.1 (2011) 116-126. https://doi.org/10.1002/bit.22914
  17. Jun, Indong, et al. Creating Hierarchical Topographies on Fibrous Platforms Using Femtosecond Laser Ablation for Directing Myoblasts Behavior. ACS applied materials & interfaces 8.5 (2016) 3407-3417. https://doi.org/10.1021/acsami.5b11418