Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Novel Detection Technology for Glycated Hemoglobin using Gold Nanoparticles

금 나노입자를 이용한 새로운 당화혈색소의 검출 기술

  • Lee, Soo Suk (Department of Phamaceutical Engineering, Soonchunhyang University)
  • 이수석 (순천향대학교 의약공학과)
  • Received : 2016.10.12
  • Accepted : 2016.11.22
  • Published : 2016.11.30
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Abstract

We report a novel detection technology for glycated hemoglobin (HbA1c) that is measured primarily to identify the three-month average plasma glucose concentration. In enzymatic measuring of glycated hemoglobin, the generated hydrogen peroxide was then used as a reducing agent of gold (III) for the synthesis of gold (0). Gold nanoparticles obtained from this novel approach were measured by optical and piezoelectric methods. In optical method, we have developed polymer based film-type sensor cartridge filled with all the reagents for glycated hemoglobin analysis and the cartridge worked very well having the detection limit of 0.53% of glycated hemoglobin. On the other hand, quartz crystal microbalance (QCM) sensors also have been developed to determine the abilities of surface modified QCM sensors at various levels of the concentration of glycated hemoglobin to bind gold nanoparticles and limit of detection was 0.90%. Finally, despite of relatively lower sensitivities of QCM sensor and film-type optical sensor than well-plate based optical detection, these two sensors were available to measure the glycated hemoglobin level for diabetes patients and normal person.

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References

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